Restriction map for
pF1KB4239
(Linear) (Six-base) MAPSORT of: KIBB4239.seq Check: 9427 from: 1 to: 3624
KIBB4239.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AatII G_ACGT'C
Cuts at: 0 2523 3624
Size: 2523 1101
AccI GT'mk_AC
Cuts at: 0 539 3624
Size: 539 3085
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 812 1082 3507 3624
Size: 71 741 270 2425 117
Fragments arranged by size:
2425 741 270 117 71
AhdI GACnn_n'nnGTC
Cuts at: 0 2391 3624
Size: 2391 1233
ApoI r'AATT_y
Cuts at: 0 58 370 394 681 1942 2659 2968 3371
Size: 58 312 24 287 1261 717 309 403
Cuts at: 3371 3624
Size: 253
Fragments arranged by size:
1261 717 403 312 309 287 253 58
24
AseI AT'TA_AT
Cuts at: 0 1682 3624
Size: 1682 1942
BamHI G'GATC_C
Cuts at: 0 278 1836 3624
Size: 278 1558 1788
Fragments arranged by size:
1788 1558 278
BanI G'GyrC_C
Cuts at: 0 2933 3547 3624
Size: 2933 614 77
Fragments arranged by size:
2933 614 77
BanII G_rGCy'C
Cuts at: 0 2268 3624
Size: 2268 1356
BbsI GAAGACnn'nnnn_
Cuts at: 0 734 1134 2423 3624
Size: 734 400 1289 1201
Fragments arranged by size:
1289 1201 734 400
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1324 1358 1444 1478 3624
Size: 1324 34 86 34 2146
Fragments arranged by size:
2146 1324 86 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1585 3624
Size: 1585 2039
BglI GCCn_nnn'nGGC
Cuts at: 0 2931 3624
Size: 2931 693
BglII A'GATC_T
Cuts at: 0 558 3624
Size: 558 3066
Bme1580I G_kGCm'C
Cuts at: 0 296 3624
Size: 296 3328
BmrI ACTGGGnnnn_n'
Cuts at: 0 352 1102 2263 3091 3624
Size: 352 750 1161 828 533
Fragments arranged by size:
1161 828 750 533 352
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 915 3042 3624
Size: 915 2127 582
Fragments arranged by size:
2127 915 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1417 1674 1922 2114 2420 2432 3624
Size: 1417 257 248 192 306 12 1192
Fragments arranged by size:
1417 1192 306 257 248 192 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1796 2997 3221 3624
Size: 799 997 1201 224 403
Fragments arranged by size:
1201 997 799 403 224
BsaI GGTCTCn'nnnn_
Cuts at: 0 3503 3523 3624
Size: 3503 20 101
Fragments arranged by size:
3503 101 20
BsaBI GATnn'nnATC
Cuts at: 0 2138 3356 3624
Size: 2138 1218 268
Fragments arranged by size:
2138 1218 268
BsaHI Gr'CG_yC
Cuts at: 0 2320 2520 3624
Size: 2320 200 1104
Fragments arranged by size:
2320 1104 200
BsaWI w'CCGG_w
Cuts at: 0 1030 1833 3624
Size: 1030 803 1791
Fragments arranged by size:
1791 1030 803
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2255 2285 3036 3066 3624
Size: 2255 30 751 30 558
Fragments arranged by size:
2255 751 558 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 445 980 3624
Size: 445 535 2644
Fragments arranged by size:
2644 535 445
BseYI C'CCAG_C
Cuts at: 0 1465 2926 3624
Size: 1465 1461 698
Fragments arranged by size:
1465 1461 698
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 912 3624
Size: 912 2712
BsmI GAATG_Cn'
Cuts at: 0 2955 3624
Size: 2955 669
Bsp1286I G_dGCh'C
Cuts at: 0 296 2268 3624
Size: 296 1972 1356
Fragments arranged by size:
1972 1356 296
BspEI T'CCGG_A
Cuts at: 0 1833 3624
Size: 1833 1791
BspHI T'CATG_A
Cuts at: 0 2109 3624
Size: 2109 1515
BsrDI GCAATG_nn'
Cuts at: 0 603 1044 1329 1432 2305 3594 3624
Size: 603 441 285 103 873 1289 30
Fragments arranged by size:
1289 873 603 441 285 103 30
BsrGI T'GTAC_A
Cuts at: 0 642 3624
Size: 642 2982
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2276 3624
Size: 2276 1348
BstYI r'GATC_y
Cuts at: 0 278 438 558 1836 2374 3559 3574 3624
Size: 278 160 120 1278 538 1185 15 50
Fragments arranged by size:
1278 1185 538 278 160 120 50 15
BstZ17I GTA'TAC
Cuts at: 0 540 3624
Size: 540 3084
Bsu36I CC'TnA_GG
Cuts at: 0 1352 3033 3624
Size: 1352 1681 591
Fragments arranged by size:
1681 1352 591
BtgI C'CryG_G
Cuts at: 0 1218 2210 2445 3513 3624
Size: 1218 992 235 1068 111
Fragments arranged by size:
1218 1068 992 235 111
BtsI GCAGTG_nn'
Cuts at: 0 741 2789 3624
Size: 741 2048 835
Fragments arranged by size:
2048 835 741
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 813 848 3624
Size: 813 35 2776
Fragments arranged by size:
2776 813 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 3490 3624
Size: 3490 134
EarI CTCTTCn'nnn_
Cuts at: 0 15 977 1955 3278 3304 3526 3624
Size: 15 962 978 1323 26 222 98
Fragments arranged by size:
1323 978 962 222 98 26 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 812 1082 1417 1674 1922 2114 2420
Size: 71 741 270 335 257 248 192 306
Cuts at: 2420 2432 3507 3624
Size: 12 1075 117
Fragments arranged by size:
1075 741 335 306 270 257 248 192
117 71 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 467 3431 3624
Size: 467 2964 193
Fragments arranged by size:
2964 467 193
EcoRV GAT'ATC
Cuts at: 0 3065 3624
Size: 3065 559
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2732 3457 3624
Size: 2732 725 167
Fragments arranged by size:
2732 725 167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 14 46 1453 1485 2377 2409 3214 3246
Size: 14 32 1407 32 892 32 805 32
Cuts at: 3246 3559 3591 3624
Size: 313 32 33
Fragments arranged by size:
1407 892 805 313 33 32 32 32
32 32 14
HincII GTy'rAC
Cuts at: 0 672 1113 2492 3624
Size: 672 441 1379 1132
Fragments arranged by size:
1379 1132 672 441
HpaI GTT'AAC
Cuts at: 0 672 3624
Size: 672 2952
MfeI C'AATT_G
Cuts at: 0 71 443 3624
Size: 71 372 3181
Fragments arranged by size:
3181 372 71
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 2546 3624
Size: 127 2419 1078
Fragments arranged by size:
2419 1078 127
MslI CAynn'nnrTG
Cuts at: 0 201 1418 2062 3624
Size: 201 1217 644 1562
Fragments arranged by size:
1562 1217 644 201
MspA1I CmG'CkG
Cuts at: 0 323 2930 3624
Size: 323 2607 694
Fragments arranged by size:
2607 694 323
NcoI C'CATG_G
Cuts at: 0 1218 2210 2445 3513 3624
Size: 1218 992 235 1068 111
Fragments arranged by size:
1218 1068 992 235 111
NdeI CA'TA_TG
Cuts at: 0 1589 3624
Size: 1589 2035
PasI CC'CwG_GG
Cuts at: 0 1148 3624
Size: 1148 2476
PflMI CCAn_nnn'nTGG
Cuts at: 0 1129 2451 3624
Size: 1129 1322 1173
Fragments arranged by size:
1322 1173 1129
PfoI T'CCnGG_A
Cuts at: 0 1651 3624
Size: 1651 1973
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 942 974 3624
Size: 942 32 2650
Fragments arranged by size:
2650 942 32
PshAI GACnn'nnGTC
Cuts at: 0 1990 3515 3624
Size: 1990 1525 109
Fragments arranged by size:
1990 1525 109
PsiI TTA'TAA
Cuts at: 0 2188 2461 3624
Size: 2188 273 1163
Fragments arranged by size:
2188 1163 273
PstI C_TGCA'G
Cuts at: 0 1512 3624
Size: 1512 2112
PvuII CAG'CTG
Cuts at: 0 2930 3624
Size: 2930 694
SapI GCTCTTCn'nnn_
Cuts at: 0 977 1955 3278 3624
Size: 977 978 1323 346
Fragments arranged by size:
1323 978 977 346
ScaI AGT'ACT
Cuts at: 0 3331 3624
Size: 3331 293
SexAI A'CCwGG_T
Cuts at: 0 2485 3624
Size: 2485 1139
SfcI C'TryA_G
Cuts at: 0 1301 1496 1508 3599 3624
Size: 1301 195 12 2091 25
Fragments arranged by size:
2091 1301 195 25 12
SmlI C'TyrA_G
Cuts at: 0 778 1811 2976 3236 3624
Size: 778 1033 1165 260 388
Fragments arranged by size:
1165 1033 778 388 260
SspI AAT'ATT
Cuts at: 0 504 1245 1923 3624
Size: 504 741 678 1701
Fragments arranged by size:
1701 741 678 504
StuI AGG'CCT
Cuts at: 0 473 2231 3624
Size: 473 1758 1393
Fragments arranged by size:
1758 1393 473
StyI C'CwwG_G
Cuts at: 0 1218 1755 1995 2174 2210 2445 3513 3624
Size: 1218 537 240 179 36 235 1068 111
Fragments arranged by size:
1218 1068 537 240 235 179 111 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3110 3434 3624
Size: 3110 324 190
Fragments arranged by size:
3110 324 190
TatI w'GTAC_w
Cuts at: 0 642 2237 2467 2511 2963 3329 3624
Size: 642 1595 230 44 452 366 295
Fragments arranged by size:
1595 642 452 366 295 230 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2255 2287 3306 3338 3624
Size: 2255 32 1019 32 286
Fragments arranged by size:
2255 1019 286 32 32
XbaI T'CTAG_A
Cuts at: 0 1788 3624
Size: 1788 1836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 200 815 942 3624
Size: 200 615 127 2682
Fragments arranged by size:
2682 615 200 127
XmnI GAAnn'nnTTC
Cuts at: 0 1850 3624
Size: 1850 1774
ZraI GAC'GTC
Cuts at: 0 2521 3624
Size: 2521 1103
Enzymes that do cut:
AatII AccI AcuI AhdI ApoI AseI BamHI BanI
BanII BbsI BcgI BciVI BglI BglII Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsmI Bsp1286I BspEI BspHI BsrDI
BsrGI BstXI BstYI BstZ17I Bsu36I BtgI BtsI CspCI
DrdI EarI Eco57MI EcoNI EcoRV FalI Hin4I HincII
HpaI MfeI MmeI MslI MspA1I NcoI NdeI PasI
PflMI PfoI PpiI PshAI PsiI PstI PvuII SapI
ScaI SexAI SfcI SmlI SspI StuI StyI TaqII
TatI TstI XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BbeI BbvCI BclI BfrBI BlpI BmgBI
BmtI Bpu10I BsaAI BsiEI BsiHKAI BsiWI BsmBI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BtgZI
ClaI DraI DraIII EaeI EagI EciI EcoICRI EcoO109I
EcoRI FseI FspI FspAI HaeII HindIII KasI KpnI
MluI MscI NaeI NarI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PmlI PpuMI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI Tth111I XhoI XmaI