(Linear) (Six-base) MAPSORT of: KIBB4155.seq  Check: 3055  from: 1  to: 897



KIBB4155.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     531     897 
   Size:        128     403     366

  Fragments arranged by size: 

                 403     366     128
 
AlwNI CAG_nnn'CTG

Cuts at:      0     613     897 
   Size:        613     284
 
ApaLI G'TGCA_C

Cuts at:      0      91     897 
   Size:         91     806
 
ApoI r'AATT_y

Cuts at:      0     116     868     897 
   Size:        116     752      29

  Fragments arranged by size: 

                 752     116      29
 
AvaI C'yCGr_G

Cuts at:      0     156     897 
   Size:        156     741
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     311     897 
   Size:        311     586
 
Bme1580I G_kGCm'C

Cuts at:      0      95     855     897 
   Size:         95     760      42

  Fragments arranged by size: 

                 760      95      42
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     320     897 
   Size:        320     577
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     641     897 
   Size:        641     256
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     336     897 
   Size:        336     561
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     172     202     403     433     575     605     897 
   Size:        172      30     201      30     142      30     292

  Fragments arranged by size: 

                 292     201     172     142      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     411     897 
   Size:        411     486
 
BsiHKAI G_wGCw'C

Cuts at:      0      67      95     897 
   Size:         67      28     802

  Fragments arranged by size: 

                 802      67      28
 
Bsp1286I G_dGCh'C

Cuts at:      0      67      95     855     897 
   Size:         67      28     760      42

  Fragments arranged by size: 

                 760      67      42      28
 
BsrBI CCG'CTC

Cuts at:      0     699     897 
   Size:        699     198
 
BstEII G'GTnAC_C

Cuts at:      0     258     897 
   Size:        258     639
 
BtsI GCAGTG_nn'

Cuts at:      0      87     452     897 
   Size:         87     365     445

  Fragments arranged by size: 

                 445     365      87
 
EarI CTCTTCn'nnn_

Cuts at:      0      46     102     561     897 
   Size:         46      56     459     336

  Fragments arranged by size: 

                 459     336      56      46
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     128     531     641     897 
   Size:        128     403     110     256

  Fragments arranged by size: 

                 403     256     128     110
 
EcoRI G'AATT_C

Cuts at:      0     116     868     897 
   Size:        116     752      29

  Fragments arranged by size: 

                 752     116      29
 
FspI TGC'GCA

Cuts at:      0     682     897 
   Size:        682     215
 
FspAI rTGC'GCAy

Cuts at:      0     682     897 
   Size:        682     215
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     575     589     607     621     897 
   Size:        575      14      18      14     276

  Fragments arranged by size: 

                 575     276      18      14      14
 
HincII GTy'rAC

Cuts at:      0     411     897 
   Size:        411     486
 
HpaI GTT'AAC

Cuts at:      0     411     897 
   Size:        411     486
 
MslI CAynn'nnrTG

Cuts at:      0     325     897 
   Size:        325     572
 
MspA1I CmG'CkG

Cuts at:      0     632     897 
   Size:        632     265
 
NspI r_CATG'y

Cuts at:      0     458     897 
   Size:        458     439
 
PvuII CAG'CTG

Cuts at:      0     632     897 
   Size:        632     265
 
SexAI A'CCwGG_T

Cuts at:      0     493     897 
   Size:        493     404
 
SfcI C'TryA_G

Cuts at:      0     404     780     897 
   Size:        404     376     117

  Fragments arranged by size: 

                 404     376     117
 
StyI C'CwwG_G

Cuts at:      0     195     897 
   Size:        195     702
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     675     897 
   Size:        675     222
 
Tth111I GACn'n_nGTC

Cuts at:      0     882     897 
   Size:        882      15
 

 Enzymes that do cut:

     AcuI    AlwNI    ApaLI     ApoI     AvaI     BbsI Bme1580I     BmrI
     BpmI     BsaI    BsaXI    BseRI  BsiHKAI Bsp1286I    BsrBI   BstEII
     BtsI     EarI  Eco57MI    EcoRI     FspI    FspAI    Hin4I   HincII
     HpaI     MslI   MspA1I     NspI    PvuII    SexAI     SfcI     StyI
    TaqII  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI    HaeII  HindIII     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI