Restriction map for
pF1KB4123
(Linear) (Six-base) MAPSORT of: KIBB4123.seq Check: 7748 from: 1 to: 1404
KIBB4123.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 0 881 1404
Size: 881 523
Acc65I G'GTAC_C
Cuts at: 0 1211 1352 1404
Size: 1211 141 52
Fragments arranged by size:
1211 141 52
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 1404
Size: 74 1330
AflIII A'CryG_T
Cuts at: 0 659 1404
Size: 659 745
AleI CACnn'nnGTG
Cuts at: 0 565 678 680 1404
Size: 565 113 2 724
Fragments arranged by size:
724 565 113 2
ApaI G_GGCC'C
Cuts at: 0 1298 1404
Size: 1298 106
ApoI r'AATT_y
Cuts at: 0 1111 1404
Size: 1111 293
AvaI C'yCGr_G
Cuts at: 0 137 194 1404
Size: 137 57 1210
Fragments arranged by size:
1210 137 57
BanI G'GyrC_C
Cuts at: 0 108 232 1211 1352 1404
Size: 108 124 979 141 52
Fragments arranged by size:
979 141 124 108 52
BanII G_rGCy'C
Cuts at: 0 713 1053 1298 1404
Size: 713 340 245 106
Fragments arranged by size:
713 340 245 106
BbsI GAAGACnn'nnnn_
Cuts at: 0 643 1397 1404
Size: 643 754 7
Fragments arranged by size:
754 643 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 994 1028 1404
Size: 994 34 376
Fragments arranged by size:
994 376 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 507 1404
Size: 507 897
BfrBI ATG'CAT
Cuts at: 0 890 1404
Size: 890 514
BglI GCCn_nnn'nGGC
Cuts at: 0 29 1404
Size: 29 1375
BglII A'GATC_T
Cuts at: 0 575 1404
Size: 575 829
BlpI GC'TnA_GC
Cuts at: 0 508 1404
Size: 508 896
Bme1580I G_kGCm'C
Cuts at: 0 1298 1404
Size: 1298 106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 1067 1404
Size: 3 1064 337
Fragments arranged by size:
1064 337 3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 787 1404
Size: 787 617
BsaI GGTCTCn'nnnn_
Cuts at: 0 861 1404
Size: 861 543
BsaBI GATnn'nnATC
Cuts at: 0 918 1404
Size: 918 486
BsaWI w'CCGG_w
Cuts at: 0 728 1208 1404
Size: 728 480 196
Fragments arranged by size:
728 480 196
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 570 1404
Size: 570 834
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1390 1404
Size: 1390 14
BsiHKAI G_wGCw'C
Cuts at: 0 736 1404
Size: 736 668
BsmI GAATG_Cn'
Cuts at: 0 890 1272 1404
Size: 890 382 132
Fragments arranged by size:
890 382 132
Bsp1286I G_dGCh'C
Cuts at: 0 713 736 1053 1298 1404
Size: 713 23 317 245 106
Fragments arranged by size:
713 317 245 106 23
BspHI T'CATG_A
Cuts at: 0 369 1262 1404
Size: 369 893 142
Fragments arranged by size:
893 369 142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 503 1404
Size: 503 901
BsrGI T'GTAC_A
Cuts at: 0 401 1404
Size: 401 1003
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1347 1404
Size: 1347 57
BstBI TT'CG_AA
Cuts at: 0 591 1404
Size: 591 813
BstYI r'GATC_y
Cuts at: 0 575 1404
Size: 575 829
Bsu36I CC'TnA_GG
Cuts at: 0 1316 1404
Size: 1316 88
BtgI C'CryG_G
Cuts at: 0 872 1404
Size: 872 532
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 699 1404
Size: 699 705
DraIII CAC_nnn'GTG
Cuts at: 0 342 1404
Size: 342 1062
EarI CTCTTCn'nnn_
Cuts at: 0 165 325 346 1404
Size: 165 160 21 1058
Fragments arranged by size:
1058 165 160 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 220 1404
Size: 220 1184
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3 74 1067 1404
Size: 3 71 993 337
Fragments arranged by size:
993 337 71 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1231 1404
Size: 1231 173
EcoO109I rG'GnC_Cy
Cuts at: 0 1310 1404
Size: 1310 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 177 209 357 389 1404
Size: 177 32 148 32 1015
Fragments arranged by size:
1015 177 148 32 32
KpnI G_GTAC'C
Cuts at: 0 1215 1356 1404
Size: 1215 141 48
Fragments arranged by size:
1215 141 48
MslI CAynn'nnrTG
Cuts at: 0 565 678 680 1303 1404
Size: 565 113 2 623 101
Fragments arranged by size:
623 565 113 101 2
MspA1I CmG'CkG
Cuts at: 0 258 565 1404
Size: 258 307 839
Fragments arranged by size:
839 307 258
NcoI C'CATG_G
Cuts at: 0 872 1404
Size: 872 532
NdeI CA'TA_TG
Cuts at: 0 886 1095 1404
Size: 886 209 309
Fragments arranged by size:
886 309 209
NsiI A_TGCA'T
Cuts at: 0 892 1404
Size: 892 512
PspOMI G'GGCC_C
Cuts at: 0 1294 1404
Size: 1294 110
SapI GCTCTTCn'nnn_
Cuts at: 0 325 1404
Size: 325 1079
SfcI C'TryA_G
Cuts at: 0 312 1404
Size: 312 1092
SmlI C'TyrA_G
Cuts at: 0 802 1404
Size: 802 602
SspI AAT'ATT
Cuts at: 0 163 688 1404
Size: 163 525 716
Fragments arranged by size:
716 525 163
StuI AGG'CCT
Cuts at: 0 929 1404
Size: 929 475
StyI C'CwwG_G
Cuts at: 0 720 872 1404
Size: 720 152 532
Fragments arranged by size:
720 532 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 334 1404
Size: 334 1070
TatI w'GTAC_w
Cuts at: 0 401 425 821 1404
Size: 401 24 396 583
Fragments arranged by size:
583 401 396 24
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI ApaI ApoI AvaI
BanI BanII BbsI BcgI BciVI BfrBI BglI BglII
BlpI Bme1580I BpmI BpuEI BsaI BsaBI BsaWI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI DraIII EarI EciI
Eco57MI EcoNI EcoO109I Hin4I KpnI MslI MspA1I NcoI
NdeI NsiI PspOMI SapI SfcI SmlI SspI StuI
StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BclI BmgBI BmrI BmtI BplI
Bpu10I BsaAI BsaHI BsaXI BseYI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BsrFI BssHII BssSI BstEII BstXI
BstZ17I BtsI ClaI CspCI DraI DrdI EaeI EagI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI MmeI MscI
NaeI NarI NgoMIV NheI NotI NruI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI