Restriction map for
pF1KB4122
(Linear) (Six-base) MAPSORT of: KIBB4122.seq Check: 7269 from: 1 to: 1842
KIBB4122.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AccI GT'mk_AC
Cuts at: 0 592 1659 1842
Size: 592 1067 183
Fragments arranged by size:
1067 592 183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 925 1399 1842
Size: 890 35 474 443
Fragments arranged by size:
890 474 443 35
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 575 1842
Size: 575 1267
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1724 1756 1842
Size: 1724 32 86
Fragments arranged by size:
1724 86 32
ApaI G_GGCC'C
Cuts at: 0 83 1842
Size: 83 1759
ApaLI G'TGCA_C
Cuts at: 0 50 908 1842
Size: 50 858 934
Fragments arranged by size:
934 858 50
ApoI r'AATT_y
Cuts at: 0 157 168 282 336 1156 1231 1842
Size: 157 11 114 54 820 75 611
Fragments arranged by size:
820 611 157 114 75 54 11
AseI AT'TA_AT
Cuts at: 0 1400 1842
Size: 1400 442
AvaI C'yCGr_G
Cuts at: 0 527 1842
Size: 527 1315
BanI G'GyrC_C
Cuts at: 0 519 1842
Size: 519 1323
BanII G_rGCy'C
Cuts at: 0 83 1842
Size: 83 1759
BbsI GAAGACnn'nnnn_
Cuts at: 0 684 1433 1842
Size: 684 749 409
Fragments arranged by size:
749 684 409
BciVI GTATCCnnnnn_n'
Cuts at: 0 1120 1842
Size: 1120 722
BfrBI ATG'CAT
Cuts at: 0 1780 1842
Size: 1780 62
Bme1580I G_kGCm'C
Cuts at: 0 54 83 912 1842
Size: 54 29 829 930
Fragments arranged by size:
930 829 54 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 1152 1842
Size: 1152 690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1188 1842
Size: 1188 654
Bpu10I CC'TnA_GC
Cuts at: 0 365 1842
Size: 365 1477
BsaI GGTCTCn'nnnn_
Cuts at: 0 17 1842
Size: 17 1825
BsaAI yAC'GTr
Cuts at: 0 634 1842
Size: 634 1208
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1243 1273 1842
Size: 1243 30 569
Fragments arranged by size:
1243 569 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1098 1251 1472 1842
Size: 1098 153 221 370
Fragments arranged by size:
1098 370 221 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1784 1842
Size: 568 1216 58
Fragments arranged by size:
1216 568 58
BsiEI CG_ry'CG
Cuts at: 0 33 1842
Size: 33 1809
BsiHKAI G_wGCw'C
Cuts at: 0 54 185 615 912 1842
Size: 54 131 430 297 930
Fragments arranged by size:
930 430 297 131 54
BsmI GAATG_Cn'
Cuts at: 0 761 1842
Size: 761 1081
Bsp1286I G_dGCh'C
Cuts at: 0 54 83 185 615 912 1842
Size: 54 29 102 430 297 930
Fragments arranged by size:
930 430 297 102 54 29
BspHI T'CATG_A
Cuts at: 0 960 1007 1842
Size: 960 47 835
Fragments arranged by size:
960 835 47
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1786 1842
Size: 1786 56
BstYI r'GATC_y
Cuts at: 0 421 1842
Size: 421 1421
BstZ17I GTA'TAC
Cuts at: 0 1660 1842
Size: 1660 182
Bsu36I CC'TnA_GG
Cuts at: 0 617 1842
Size: 617 1225
BtgI C'CryG_G
Cuts at: 0 27 1103 1113 1842
Size: 27 1076 10 729
Fragments arranged by size:
1076 729 27 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 509 1842
Size: 509 1333
BtsI GCAGTG_nn'
Cuts at: 0 350 1842
Size: 350 1492
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1488 1523 1842
Size: 1488 35 319
Fragments arranged by size:
1488 319 35
EaeI y'GGCC_r
Cuts at: 0 30 373 844 1842
Size: 30 343 471 998
Fragments arranged by size:
998 471 343 30
EagI C'GGCC_G
Cuts at: 0 30 1842
Size: 30 1812
EarI CTCTTCn'nnn_
Cuts at: 0 995 1842
Size: 995 847
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 925 1188 1399 1842
Size: 890 35 263 211 443
Fragments arranged by size:
890 443 263 211 35
EcoO109I rG'GnC_Cy
Cuts at: 0 79 80 1842
Size: 79 1 1762
Fragments arranged by size:
1762 79 1
EcoRI G'AATT_C
Cuts at: 0 1156 1842
Size: 1156 686
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 783 815 1186 1218 1354 1386 1474 1506
Size: 783 32 371 32 136 32 88 32
Cuts at: 1506 1668 1700 1800 1832 1842
Size: 162 32 100 32 10
Fragments arranged by size:
783 371 162 136 100 88 32 32
32 32 32 32 10
HincII GTy'rAC
Cuts at: 0 1129 1842
Size: 1129 713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 384 1711 1842
Size: 384 1327 131
Fragments arranged by size:
1327 384 131
MscI TGG'CCA
Cuts at: 0 375 846 1842
Size: 375 471 996
Fragments arranged by size:
996 471 375
MspA1I CmG'CkG
Cuts at: 0 29 1798 1842
Size: 29 1769 44
Fragments arranged by size:
1769 44 29
NcoI C'CATG_G
Cuts at: 0 1103 1113 1842
Size: 1103 10 729
Fragments arranged by size:
1103 729 10
NdeI CA'TA_TG
Cuts at: 0 1776 1842
Size: 1776 66
NsiI A_TGCA'T
Cuts at: 0 1782 1842
Size: 1782 60
NspI r_CATG'y
Cuts at: 0 581 1842
Size: 581 1261
PasI CC'CwG_GG
Cuts at: 0 109 1842
Size: 109 1733
PshAI GACnn'nnGTC
Cuts at: 0 1124 1842
Size: 1124 718
PspOMI G'GGCC_C
Cuts at: 0 79 1842
Size: 79 1763
PstI C_TGCA'G
Cuts at: 0 1797 1842
Size: 1797 45
PvuII CAG'CTG
Cuts at: 0 1798 1842
Size: 1798 44
SacII CC_GC'GG
Cuts at: 0 30 1842
Size: 30 1812
SfcI C'TryA_G
Cuts at: 0 1190 1793 1842
Size: 1190 603 49
Fragments arranged by size:
1190 603 49
SspI AAT'ATT
Cuts at: 0 568 1842
Size: 568 1274
StyI C'CwwG_G
Cuts at: 0 1103 1113 1842
Size: 1103 10 729
Fragments arranged by size:
1103 729 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 35 53 1082 1842
Size: 35 18 1029 760
Fragments arranged by size:
1029 760 35 18
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1038 1842
Size: 1038 804
Enzymes that do cut:
AccI AcuI AlfI AloI ApaI ApaLI ApoI AseI
AvaI BanI BanII BbsI BciVI BfrBI Bme1580I BmrI
BpmI Bpu10I BsaI BsaAI BsaXI BseRI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BstAPI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI CspCI EaeI EagI EarI Eco57MI
EcoO109I EcoRI Hin4I HincII MmeI MscI MspA1I NcoI
NdeI NsiI NspI PasI PshAI PspOMI PstI PvuII
SacII SfcI SspI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlwNI AscI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BclI BglI BglII BlpI BmgBI
BmtI BplI BpuEI BsaBI BsaHI BsaWI BseYI BsiWI
BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI ClaI DraI DraIII DrdI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NgoMIV NheI NotI NruI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI