Restriction map for
pF1KB4102
(Linear) (Six-base) MAPSORT of: KIBB4102.seq Check: 792 from: 1 to: 2145
KIBB4102.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1679 2145
Size: 1679 466
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 1277 2145
Size: 311 966 868
Fragments arranged by size:
966 868 311
AflIII A'CryG_T
Cuts at: 0 17 1838 2145
Size: 17 1821 307
Fragments arranged by size:
1821 307 17
AleI CACnn'nnGTG
Cuts at: 0 739 1411 2073 2145
Size: 739 672 662 72
Fragments arranged by size:
739 672 662 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1102 2145
Size: 1102 1043
AlwNI CAG_nnn'CTG
Cuts at: 0 174 285 781 829 889 1122 1255 1681
Size: 174 111 496 48 60 233 133 426
Cuts at: 1681 2145
Size: 464
Fragments arranged by size:
496 464 426 233 174 133 111 60
48
ApoI r'AATT_y
Cuts at: 0 2116 2145
Size: 2116 29
BanI G'GyrC_C
Cuts at: 0 727 1014 1372 2145
Size: 727 287 358 773
Fragments arranged by size:
773 727 358 287
BanII G_rGCy'C
Cuts at: 0 38 2145
Size: 38 2107
BbeI G_GCGC'C
Cuts at: 0 1376 2145
Size: 1376 769
BbsI GAAGACnn'nnnn_
Cuts at: 0 1503 2145
Size: 1503 642
BbvCI CC'TCA_GC
Cuts at: 0 822 890 1075 2145
Size: 822 68 185 1070
Fragments arranged by size:
1070 822 185 68
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1431 1465 1548 1582 2090 2124 2145
Size: 1431 34 83 34 508 34 21
Fragments arranged by size:
1431 508 83 34 34 34 21
BfrBI ATG'CAT
Cuts at: 0 1971 2145
Size: 1971 174
BglI GCCn_nnn'nGGC
Cuts at: 0 115 170 214 562 1095 1312 2145
Size: 115 55 44 348 533 217 833
Fragments arranged by size:
833 533 348 217 115 55 44
BglII A'GATC_T
Cuts at: 0 1730 2145
Size: 1730 415
Bme1580I G_kGCm'C
Cuts at: 0 719 905 1185 1535 1561 2145
Size: 719 186 280 350 26 584
Fragments arranged by size:
719 584 350 280 186 26
BmgBI CAC'GTC
Cuts at: 0 1839 2145
Size: 1839 306
BmrI ACTGGGnnnn_n'
Cuts at: 0 440 1566 2067 2145
Size: 440 1126 501 78
Fragments arranged by size:
1126 501 440 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1977 2082 2145
Size: 1977 105 63
Fragments arranged by size:
1977 105 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 196 262 318 651 672 1144 2145
Size: 175 21 66 56 333 21 472 1001
Fragments arranged by size:
1001 472 333 175 66 56 21 21
Bpu10I CC'TnA_GC
Cuts at: 0 822 890 955 1075 1708 2145
Size: 822 68 65 120 633 437
Fragments arranged by size:
822 633 437 120 68 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1345 1807 2145
Size: 1345 462 338
Fragments arranged by size:
1345 462 338
BsaI GGTCTCn'nnnn_
Cuts at: 0 58 298 539 2056 2145
Size: 58 240 241 1517 89
Fragments arranged by size:
1517 241 240 89 58
BsaAI yAC'GTr
Cuts at: 0 141 2145
Size: 141 2004
BsaHI Gr'CG_yC
Cuts at: 0 1163 1373 2145
Size: 1163 210 772
Fragments arranged by size:
1163 772 210
BsaWI w'CCGG_w
Cuts at: 0 296 2145
Size: 296 1849
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1514 1544 2145
Size: 1514 30 601
Fragments arranged by size:
1514 601 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 615 889 1039 1237 1348 2071 2074 2145
Size: 615 274 150 198 111 723 3 71
Fragments arranged by size:
723 615 274 198 150 111 71 3
BseYI C'CCAG_C
Cuts at: 0 418 1166 2127 2140 2145
Size: 418 748 961 13 5
Fragments arranged by size:
961 748 418 13 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1401 1651 2145
Size: 1401 250 494
Fragments arranged by size:
1401 494 250
BsiHKAI G_wGCw'C
Cuts at: 0 1553 2145
Size: 1553 592
BsmBI CGTCTCn'nnnn_
Cuts at: 0 124 2145
Size: 124 2021
Bsp1286I G_dGCh'C
Cuts at: 0 38 719 905 1185 1535 1553 1561 2145
Size: 38 681 186 280 350 18 8 584
Fragments arranged by size:
681 584 350 280 186 38 18 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 164 1032 1089 1655 1673 1679 2006 2033
Size: 164 868 57 566 18 6 327 27
Cuts at: 2033 2145
Size: 112
Fragments arranged by size:
868 566 327 164 112 57 27 18
6
BsrBI CCG'CTC
Cuts at: 0 26 429 2145
Size: 26 403 1716
Fragments arranged by size:
1716 403 26
BsrFI r'CCGG_y
Cuts at: 0 1017 2012 2145
Size: 1017 995 133
Fragments arranged by size:
1017 995 133
BsrGI T'GTAC_A
Cuts at: 0 219 2145
Size: 219 1926
BssHII G'CGCG_C
Cuts at: 0 8 92 118 2145
Size: 8 84 26 2027
Fragments arranged by size:
2027 84 26 8
BstXI CCAn_nnnn'nTGG
Cuts at: 0 45 2145
Size: 45 2100
BstYI r'GATC_y
Cuts at: 0 485 1730 2145
Size: 485 1245 415
Fragments arranged by size:
1245 485 415
Bsu36I CC'TnA_GG
Cuts at: 0 1092 2145
Size: 1092 1053
BtgI C'CryG_G
Cuts at: 0 56 1505 1517 2145
Size: 56 1449 12 628
Fragments arranged by size:
1449 628 56 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1902 2145
Size: 1902 243
BtsI GCAGTG_nn'
Cuts at: 0 129 1342 1535 2145
Size: 129 1213 193 610
Fragments arranged by size:
1213 610 193 129
DrdI GACnn_nn'nnGTC
Cuts at: 0 1690 2145
Size: 1690 455
EaeI y'GGCC_r
Cuts at: 0 107 579 977 1019 1502 1853 2145
Size: 107 472 398 42 483 351 292
Fragments arranged by size:
483 472 398 351 292 107 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 2145
Size: 46 2099
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 196 262 311 318 651 672 1144
Size: 175 21 66 49 7 333 21 472
Cuts at: 1144 1277 2145
Size: 133 868
Fragments arranged by size:
868 472 333 175 133 66 49 21
21 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 178 2053 2145
Size: 178 1875 92
Fragments arranged by size:
1875 178 92
EcoO109I rG'GnC_Cy
Cuts at: 0 171 322 555 1044 1071 2145
Size: 171 151 233 489 27 1074
Fragments arranged by size:
1074 489 233 171 151 27
EcoRI G'AATT_C
Cuts at: 0 2116 2145
Size: 2116 29
FspI TGC'GCA
Cuts at: 0 1783 1948 2145
Size: 1783 165 197
Fragments arranged by size:
1783 197 165
FspAI rTGC'GCAy
Cuts at: 0 1783 2145
Size: 1783 362
HaeII r_GCGC'y
Cuts at: 0 543 1376 2145
Size: 543 833 769
Fragments arranged by size:
833 769 543
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 1279 1311 2145
Size: 295 32 952 32 834
Fragments arranged by size:
952 834 295 32 32
KasI G'GCGC_C
Cuts at: 0 1372 2145
Size: 1372 773
MluI A'CGCG_T
Cuts at: 0 17 2145
Size: 17 2128
MscI TGG'CCA
Cuts at: 0 581 979 1504 2145
Size: 581 398 525 641
Fragments arranged by size:
641 581 525 398
MslI CAynn'nnrTG
Cuts at: 0 232 377 739 991 1411 1721 1882 1906
Size: 232 145 362 252 420 310 161 24
Cuts at: 1906 2073 2145
Size: 167 72
Fragments arranged by size:
420 362 310 252 232 167 161 145
72 24
MspA1I CmG'CkG
Cuts at: 0 58 620 692 889 1211 1626 1678 1861
Size: 58 562 72 197 322 415 52 183
Cuts at: 1861 2145
Size: 284
Fragments arranged by size:
562 415 322 284 197 183 72 58
52
NaeI GCC'GGC
Cuts at: 0 1019 2145
Size: 1019 1126
NarI GG'CG_CC
Cuts at: 0 1373 2145
Size: 1373 772
NcoI C'CATG_G
Cuts at: 0 1505 1517 2145
Size: 1505 12 628
Fragments arranged by size:
1505 628 12
NgoMIV G'CCGG_C
Cuts at: 0 1017 2145
Size: 1017 1128
NsiI A_TGCA'T
Cuts at: 0 1973 2145
Size: 1973 172
NspI r_CATG'y
Cuts at: 0 1452 1975 2145
Size: 1452 523 170
Fragments arranged by size:
1452 523 170
PasI CC'CwG_GG
Cuts at: 0 846 2145
Size: 846 1299
PflMI CCAn_nnn'nTGG
Cuts at: 0 375 2145
Size: 375 1770
PpuMI rG'GwC_Cy
Cuts at: 0 1044 1071 2145
Size: 1044 27 1074
Fragments arranged by size:
1074 1044 27
PstI C_TGCA'G
Cuts at: 0 170 390 795 1835 2003 2030 2145
Size: 170 220 405 1040 168 27 115
Fragments arranged by size:
1040 405 220 170 168 115 27
PvuII CAG'CTG
Cuts at: 0 692 889 1626 1678 1861 2145
Size: 692 197 737 52 183 284
Fragments arranged by size:
737 692 284 197 183 52
SacII CC_GC'GG
Cuts at: 0 59 2145
Size: 59 2086
SbfI CC_TGCA'GG
Cuts at: 0 170 1835 2030 2145
Size: 170 1665 195 115
Fragments arranged by size:
1665 195 170 115
SfcI C'TryA_G
Cuts at: 0 166 386 791 1430 1831 1976 1999 2026
Size: 166 220 405 639 401 145 23 27
Cuts at: 2026 2145
Size: 119
Fragments arranged by size:
639 405 401 220 166 145 119 27
23
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 562 1312 2145
Size: 562 750 833
Fragments arranged by size:
833 750 562
SfoI GGC'GCC
Cuts at: 0 1374 2145
Size: 1374 771
SmlI C'TyrA_G
Cuts at: 0 1360 1822 2145
Size: 1360 462 323
Fragments arranged by size:
1360 462 323
SphI G_CATG'C
Cuts at: 0 1975 2145
Size: 1975 170
StuI AGG'CCT
Cuts at: 0 476 1009 2145
Size: 476 533 1136
Fragments arranged by size:
1136 533 476
StyI C'CwwG_G
Cuts at: 0 767 1307 1505 1517 1607 2145
Size: 767 540 198 12 90 538
Fragments arranged by size:
767 540 538 198 90 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 528 714 745 2145
Size: 528 186 31 1400
Fragments arranged by size:
1400 528 186 31
TatI w'GTAC_w
Cuts at: 0 219 251 2145
Size: 219 32 1894
Fragments arranged by size:
1894 219 32
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1514 1546 1802 1834 2145
Size: 1514 32 256 32 311
Fragments arranged by size:
1514 311 256 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 309 2145
Size: 309 1836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1383 2145
Size: 1383 762
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApoI BanI
BanII BbeI BbsI BbvCI BcgI BfrBI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I
KasI MluI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PasI PflMI PpuMI PstI PvuII
SacII SbfI SfcI SfiI SfoI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BciVI BclI BlpI BmtI BsaBI BsiEI
BsiWI BsmI BspEI BspHI BsrDI BssSI BstAPI BstBI
BstEII BstZ17I ClaI CspCI DraI DraIII EagI EarI
EcoICRI EcoRV FalI FseI HincII HindIII HpaI KpnI
MfeI MmeI NdeI NheI NotI NruI PacI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SalI SanDI SapI ScaI
SexAI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI XmaI XmnI ZraI