Restriction map for
pF1KB4096
(Linear) (Six-base) MAPSORT of: KIBB4096.seq Check: 5723 from: 1 to: 2919
KIBB4096.orf
With 182 enzymes: SgfI *
January 12, 2016 10:05 ..
AccI GT'mk_AC
Cuts at: 0 560 2067 2919
Size: 560 1507 852
Fragments arranged by size:
1507 852 560
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1375 1699 2919
Size: 139 1236 324 1220
Fragments arranged by size:
1236 1220 324 139
AleI CACnn'nnGTG
Cuts at: 0 1765 2919
Size: 1765 1154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 73 2919
Size: 73 2846
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 388 420 691 723 2919
Size: 388 32 271 32 2196
Fragments arranged by size:
2196 388 271 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2795 2919
Size: 2795 124
ApoI r'AATT_y
Cuts at: 0 412 1555 1783 2154 2190 2223 2551 2805
Size: 412 1143 228 371 36 33 328 254
Cuts at: 2805 2919
Size: 114
Fragments arranged by size:
1143 412 371 328 254 228 114 36
33
AvaI C'yCGr_G
Cuts at: 0 2369 2919
Size: 2369 550
BamHI G'GATC_C
Cuts at: 0 1664 2919
Size: 1664 1255
BanI G'GyrC_C
Cuts at: 0 1415 1963 2464 2919
Size: 1415 548 501 455
Fragments arranged by size:
1415 548 501 455
BanII G_rGCy'C
Cuts at: 0 86 680 689 731 784 2370 2919
Size: 86 594 9 42 53 1586 549
Fragments arranged by size:
1586 594 549 86 53 42 9
BfrBI ATG'CAT
Cuts at: 0 295 2919
Size: 295 2624
BglII A'GATC_T
Cuts at: 0 167 1520 2919
Size: 167 1353 1399
Fragments arranged by size:
1399 1353 167
BlpI GC'TnA_GC
Cuts at: 0 346 688 908 2919
Size: 346 342 220 2011
Fragments arranged by size:
2011 346 342 220
Bme1580I G_kGCm'C
Cuts at: 0 219 2469 2919
Size: 219 2250 450
Fragments arranged by size:
2250 450 219
BmrI ACTGGGnnnn_n'
Cuts at: 0 987 1988 2542 2570 2919
Size: 987 1001 554 28 349
Fragments arranged by size:
1001 987 554 349 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 747 2919
Size: 747 2172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 768 814 2235 2919
Size: 268 500 46 1421 684
Fragments arranged by size:
1421 684 500 268 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 2106 2509 2919
Size: 664 1442 403 410
Fragments arranged by size:
1442 664 410 403
BsaI GGTCTCn'nnnn_
Cuts at: 0 38 2048 2542 2919
Size: 38 2010 494 377
Fragments arranged by size:
2010 494 377 38
BsaWI w'CCGG_w
Cuts at: 0 420 2919
Size: 420 2499
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 529 559 565 595 691 721 2522 2552
Size: 529 30 6 30 96 30 1801 30
Cuts at: 2552 2919
Size: 367
Fragments arranged by size:
1801 529 367 96 30 30 30 30
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 739 2790 2814 2919
Size: 109 630 2051 24 105
Fragments arranged by size:
2051 630 109 105 24
BseYI C'CCAG_C
Cuts at: 0 361 671 2919
Size: 361 310 2248
Fragments arranged by size:
2248 361 310
BsiHKAI G_wGCw'C
Cuts at: 0 86 680 689 731 784 2919
Size: 86 594 9 42 53 2135
Fragments arranged by size:
2135 594 86 53 42 9
BsmI GAATG_Cn'
Cuts at: 0 2755 2919
Size: 2755 164
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1480 2333 2919
Size: 1480 853 586
Fragments arranged by size:
1480 853 586
Bsp1286I G_dGCh'C
Cuts at: 0 86 219 680 689 731 784 2370 2469
Size: 86 133 461 9 42 53 1586 99
Cuts at: 2469 2919
Size: 450
Fragments arranged by size:
1586 461 450 133 99 86 53 42
9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 366 770 1006 2919
Size: 366 404 236 1913
Fragments arranged by size:
1913 404 366 236
BsrDI GCAATG_nn'
Cuts at: 0 224 295 623 1328 2112 2919
Size: 224 71 328 705 784 807
Fragments arranged by size:
807 784 705 328 224 71
BsrGI T'GTAC_A
Cuts at: 0 1182 2591 2919
Size: 1182 1409 328
Fragments arranged by size:
1409 1182 328
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2750 2919
Size: 2750 169
BstXI CCAn_nnnn'nTGG
Cuts at: 0 441 792 2919
Size: 441 351 2127
Fragments arranged by size:
2127 441 351
BstYI r'GATC_y
Cuts at: 0 167 518 1520 1664 2919
Size: 167 351 1002 144 1255
Fragments arranged by size:
1255 1002 351 167 144
BtgI C'CryG_G
Cuts at: 0 505 2424 2919
Size: 505 1919 495
Fragments arranged by size:
1919 505 495
BtsI GCAGTG_nn'
Cuts at: 0 745 2364 2751 2919
Size: 745 1619 387 168
Fragments arranged by size:
1619 745 387 168
ClaI AT'CG_AT
Cuts at: 0 1640 2919
Size: 1640 1279
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1187 1222 1686 1721 2919
Size: 1187 35 464 35 1198
Fragments arranged by size:
1198 1187 464 35 35
DraI TTT'AAA
Cuts at: 0 1748 2919
Size: 1748 1171
DraIII CAC_nnn'GTG
Cuts at: 0 279 941 2919
Size: 279 662 1978
Fragments arranged by size:
1978 662 279
DrdI GACnn_nn'nnGTC
Cuts at: 0 1716 2919
Size: 1716 1203
EaeI y'GGCC_r
Cuts at: 0 860 2919
Size: 860 2059
EarI CTCTTCn'nnn_
Cuts at: 0 824 1447 1718 1729 2780 2919
Size: 824 623 271 11 1051 139
Fragments arranged by size:
1051 824 623 271 139 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 984 2919
Size: 984 1935
EcoICRI GAG'CTC
Cuts at: 0 84 678 687 729 782 2919
Size: 84 594 9 42 53 2137
Fragments arranged by size:
2137 594 84 53 42 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 268 768 814 1375 1699 2235 2919
Size: 139 129 500 46 561 324 536 684
Fragments arranged by size:
684 561 536 500 324 139 129 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 157 2704 2919
Size: 157 2547 215
Fragments arranged by size:
2547 215 157
EcoO109I rG'GnC_Cy
Cuts at: 0 150 378 642 1189 1510 2515 2919
Size: 150 228 264 547 321 1005 404
Fragments arranged by size:
1005 547 404 321 264 228 150
EcoRI G'AATT_C
Cuts at: 0 412 1783 2919
Size: 412 1371 1136
Fragments arranged by size:
1371 1136 412
EcoRV GAT'ATC
Cuts at: 0 1548 1908 2919
Size: 1548 360 1011
Fragments arranged by size:
1548 1011 360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1104 2919
Size: 1104 1815
HaeII r_GCGC'y
Cuts at: 0 332 2735 2919
Size: 332 2403 184
Fragments arranged by size:
2403 332 184
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 35 67 529 561 578 610 2112 2144
Size: 35 32 462 32 17 32 1502 32
Cuts at: 2144 2919
Size: 775
Fragments arranged by size:
1502 775 462 35 32 32 32 32
17
HincII GTy'rAC
Cuts at: 0 27 183 2919
Size: 27 156 2736
Fragments arranged by size:
2736 156 27
HindIII A'AGCT_T
Cuts at: 0 988 2919
Size: 988 1931
HpaI GTT'AAC
Cuts at: 0 183 2919
Size: 183 2736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1478 2919
Size: 1478 1441
MscI TGG'CCA
Cuts at: 0 862 2919
Size: 862 2057
MslI CAynn'nnrTG
Cuts at: 0 388 815 1765 2919
Size: 388 427 950 1154
Fragments arranged by size:
1154 950 427 388
MspA1I CmG'CkG
Cuts at: 0 365 524 2919
Size: 365 159 2395
Fragments arranged by size:
2395 365 159
NcoI C'CATG_G
Cuts at: 0 2424 2919
Size: 2424 495
NdeI CA'TA_TG
Cuts at: 0 2564 2919
Size: 2564 355
NsiI A_TGCA'T
Cuts at: 0 297 2919
Size: 297 2622
NspI r_CATG'y
Cuts at: 0 1539 1857 2919
Size: 1539 318 1062
Fragments arranged by size:
1539 1062 318
PflMI CCAn_nnn'nTGG
Cuts at: 0 858 941 2919
Size: 858 83 1978
Fragments arranged by size:
1978 858 83
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 2919
Size: 691 32 2196
Fragments arranged by size:
2196 691 32
PpuMI rG'GwC_Cy
Cuts at: 0 378 642 1510 2515 2919
Size: 378 264 868 1005 404
Fragments arranged by size:
1005 868 404 378 264
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1318 1350 1597 1629 2919
Size: 1318 32 247 32 1290
Fragments arranged by size:
1318 1290 247 32 32
PstI C_TGCA'G
Cuts at: 0 93 1520 2919
Size: 93 1427 1399
Fragments arranged by size:
1427 1399 93
SacI G_AGCT'C
Cuts at: 0 86 680 689 731 784 2919
Size: 86 594 9 42 53 2135
Fragments arranged by size:
2135 594 86 53 42 9
SapI GCTCTTCn'nnn_
Cuts at: 0 824 2919
Size: 824 2095
SfcI C'TryA_G
Cuts at: 0 89 351 1034 1516 2919
Size: 89 262 683 482 1403
Fragments arranged by size:
1403 683 482 262 89
SmlI C'TyrA_G
Cuts at: 0 679 2085 2524 2919
Size: 679 1406 439 395
Fragments arranged by size:
1406 679 439 395
SphI G_CATG'C
Cuts at: 0 1539 2919
Size: 1539 1380
StuI AGG'CCT
Cuts at: 0 103 2677 2919
Size: 103 2574 242
Fragments arranged by size:
2574 242 103
StyI C'CwwG_G
Cuts at: 0 203 1065 1225 1393 2016 2299 2424 2452
Size: 203 862 160 168 623 283 125 28
Cuts at: 2452 2469 2919
Size: 17 450
Fragments arranged by size:
862 623 450 283 203 168 160 125
28 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 1068 2919
Size: 277 791 1851
Fragments arranged by size:
1851 791 277
TatI w'GTAC_w
Cuts at: 0 11 1182 2591 2919
Size: 11 1171 1409 328
Fragments arranged by size:
1409 1171 328 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 565 597 1099 1131 1656 1688 2770 2802
Size: 565 32 502 32 525 32 1082 32
Cuts at: 2802 2919
Size: 117
Fragments arranged by size:
1082 565 525 502 117 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 390 762 870 1400 2919
Size: 390 372 108 530 1519
Fragments arranged by size:
1519 530 390 372 108
XmnI GAAnn'nnTTC
Cuts at: 0 103 706 2919
Size: 103 603 2213
Fragments arranged by size:
2213 603 103
Enzymes that do cut:
AccI AcuI AleI AlfI AloI AlwNI ApoI AvaI
BamHI BanI BanII BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaWI BsaXI BseRI BseYI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrGI BstAPI
BstXI BstYI BtgI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I HincII HindIII HpaI
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PflMI PpiI PpuMI PsrI PstI SacI SapI SfcI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BbvCI BcgI BciVI BclI BglI BmgBI
BmtI Bpu10I BsaAI BsaBI BsaHI BsgI BsiEI BsiWI
BspEI BspHI BsrBI BsrFI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI EagI FseI FspI FspAI KasI
KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PasI PciI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XhoI XmaI ZraI