Restriction map for
pF1KB4089
(Linear) (Six-base) MAPSORT of: KIBB4089.seq Check: 2873 from: 1 to: 4668
KIBB4089.orf
With 182 enzymes: SgfI *
September 19, 2018 16:07 ..
AccI GT'mk_AC
Cuts at: 0 174 3585 4668
Size: 174 3411 1083
Fragments arranged by size:
3411 1083 174
AclI AA'CG_TT
Cuts at: 0 3597 4668
Size: 3597 1071
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 703 789 1478 1661 2213 2519 2831
Size: 217 486 86 689 183 552 306 312
Cuts at: 2831 3578 4113 4668
Size: 747 535 555
Fragments arranged by size:
747 689 555 552 535 486 312 306
217 183 86
AflII C'TTAA_G
Cuts at: 0 1219 2023 4668
Size: 1219 804 2645
Fragments arranged by size:
2645 1219 804
AflIII A'CryG_T
Cuts at: 0 453 4668
Size: 453 4215
AhdI GACnn_n'nnGTC
Cuts at: 0 4262 4606 4668
Size: 4262 344 62
Fragments arranged by size:
4262 344 62
AleI CACnn'nnGTG
Cuts at: 0 1810 4668
Size: 1810 2858
AlwNI CAG_nnn'CTG
Cuts at: 0 1684 4668
Size: 1684 2984
ApaLI G'TGCA_C
Cuts at: 0 2789 4668
Size: 2789 1879
ApoI r'AATT_y
Cuts at: 0 308 1997 2085 2263 2818 4668
Size: 308 1689 88 178 555 1850
Fragments arranged by size:
1850 1689 555 308 178 88
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 172 205 4668
Size: 172 33 4463
Fragments arranged by size:
4463 172 33
BanII G_rGCy'C
Cuts at: 0 2366 4668
Size: 2366 2302
BbsI GAAGACnn'nnnn_
Cuts at: 0 2507 3104 3612 3662 3798 4668
Size: 2507 597 508 50 136 870
Fragments arranged by size:
2507 870 597 508 136 50
BbvCI CC'TCA_GC
Cuts at: 0 2840 4668
Size: 2840 1828
BciVI GTATCCnnnnn_n'
Cuts at: 0 2574 4668
Size: 2574 2094
BfrBI ATG'CAT
Cuts at: 0 1309 2887 4668
Size: 1309 1578 1781
Fragments arranged by size:
1781 1578 1309
BglII A'GATC_T
Cuts at: 0 95 2875 4165 4668
Size: 95 2780 1290 503
Fragments arranged by size:
2780 1290 503 95
Bme1580I G_kGCm'C
Cuts at: 0 2793 4668
Size: 2793 1875
BmgBI CAC'GTC
Cuts at: 0 4608 4668
Size: 4608 60
BmrI ACTGGGnnnn_n'
Cuts at: 0 1775 4270 4610 4668
Size: 1775 2495 340 58
Fragments arranged by size:
2495 1775 340 58
BmtI G_CTAG'C
Cuts at: 0 2800 4668
Size: 2800 1868
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3371 3373 4668
Size: 3371 2 1295
Fragments arranged by size:
3371 1295 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2597 3036 4223 4668
Size: 2597 439 1187 445
Fragments arranged by size:
2597 1187 445 439
Bpu10I CC'TnA_GC
Cuts at: 0 2840 4668
Size: 2840 1828
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 2534 3529 3740 3831 4668
Size: 223 2311 995 211 91 837
Fragments arranged by size:
2311 995 837 223 211 91
BsaI GGTCTCn'nnnn_
Cuts at: 0 825 3618 4668
Size: 825 2793 1050
Fragments arranged by size:
2793 1050 825
BsaAI yAC'GTr
Cuts at: 0 1144 2626 4668
Size: 1144 1482 2042
Fragments arranged by size:
2042 1482 1144
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3159 3189 3709 3739 4178 4208 4668
Size: 3159 30 520 30 439 30 460
Fragments arranged by size:
3159 520 460 439 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1742 3603 3730 3953 4304 4668
Size: 1742 1861 127 223 351 364
Fragments arranged by size:
1861 1742 364 351 223 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1538 1623 1648 2659 2930 4668
Size: 1538 85 25 1011 271 1738
Fragments arranged by size:
1738 1538 1011 271 85 25
BsiEI CG_ry'CG
Cuts at: 0 2424 4668
Size: 2424 2244
BsiHKAI G_wGCw'C
Cuts at: 0 2366 2793 3904 4668
Size: 2366 427 1111 764
Fragments arranged by size:
2366 1111 764 427
BsmI GAATG_Cn'
Cuts at: 0 3447 4668
Size: 3447 1221
Bsp1286I G_dGCh'C
Cuts at: 0 2366 2793 3904 4668
Size: 2366 427 1111 764
Fragments arranged by size:
2366 1111 764 427
BspHI T'CATG_A
Cuts at: 0 4296 4668
Size: 4296 372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 59 4668
Size: 59 4609
BsrBI CCG'CTC
Cuts at: 0 3276 4668
Size: 3276 1392
BsrDI GCAATG_nn'
Cuts at: 0 3248 4136 4418 4668
Size: 3248 888 282 250
Fragments arranged by size:
3248 888 282 250
BsrFI r'CCGG_y
Cuts at: 0 4644 4668
Size: 4644 24
BsrGI T'GTAC_A
Cuts at: 0 2541 4668
Size: 2541 2127
BssSI C'ACGA_G
Cuts at: 0 659 4668
Size: 659 4009
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1561 4668
Size: 1561 3107
BstBI TT'CG_AA
Cuts at: 0 4507 4668
Size: 4507 161
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2672 4018 4623 4668
Size: 2672 1346 605 45
Fragments arranged by size:
2672 1346 605 45
BstYI r'GATC_y
Cuts at: 0 95 1626 2553 2875 4165 4668
Size: 95 1531 927 322 1290 503
Fragments arranged by size:
1531 1290 927 503 322 95
Bsu36I CC'TnA_GG
Cuts at: 0 2558 4668
Size: 2558 2110
BtgI C'CryG_G
Cuts at: 0 44 4668
Size: 44 4624
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3488 3707 4232 4668
Size: 3488 219 525 436
Fragments arranged by size:
3488 525 436 219
BtsI GCAGTG_nn'
Cuts at: 0 403 3228 4024 4668
Size: 403 2825 796 644
Fragments arranged by size:
2825 796 644 403
ClaI AT'CG_AT
Cuts at: 0 342 4668
Size: 342 4326
DraI TTT'AAA
Cuts at: 0 2259 4668
Size: 2259 2409
DraIII CAC_nnn'GTG
Cuts at: 0 1411 4668
Size: 1411 3257
EaeI y'GGCC_r
Cuts at: 0 1588 3855 4278 4668
Size: 1588 2267 423 390
Fragments arranged by size:
2267 1588 423 390
EarI CTCTTCn'nnn_
Cuts at: 0 819 1067 1460 2471 2807 3101 3716 3755
Size: 819 248 393 1011 336 294 615 39
Cuts at: 3755 3843 4668
Size: 88 825
Fragments arranged by size:
1011 825 819 615 393 336 294 248
88 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 91 4668
Size: 91 4577
EcoICRI GAG'CTC
Cuts at: 0 2364 4668
Size: 2364 2304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 703 789 1478 1661 2213 2519 2597
Size: 217 486 86 689 183 552 306 78
Cuts at: 2597 2831 3036 3578 4113 4223 4668
Size: 234 205 542 535 110 445
Fragments arranged by size:
689 552 542 535 486 445 306 234
217 205 183 110 86 78
EcoO109I rG'GnC_Cy
Cuts at: 0 4386 4668
Size: 4386 282
EcoRI G'AATT_C
Cuts at: 0 1997 4668
Size: 1997 2671
EcoRV GAT'ATC
Cuts at: 0 321 1717 4668
Size: 321 1396 2951
Fragments arranged by size:
2951 1396 321
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 539 1123 2038 3169 3313 3406 3899 4668
Size: 539 584 915 1131 144 93 493 769
Fragments arranged by size:
1131 915 769 584 539 493 144 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 335 367 1652 1678 1684 1710 2197 2229
Size: 335 32 1285 26 6 26 487 32
Cuts at: 2229 3527 3559 4192 4224 4248 4280 4592 4624
Size: 1298 32 633 32 24 32 312 32
Cuts at: 4624 4668
Size: 44
Fragments arranged by size:
1298 1285 633 487 335 312 44 32
32 32 32 32 32 26 26 24
6
HincII GTy'rAC
Cuts at: 0 459 3586 4668
Size: 459 3127 1082
Fragments arranged by size:
3127 1082 459
HindIII A'AGCT_T
Cuts at: 0 673 4594 4668
Size: 673 3921 74
Fragments arranged by size:
3921 673 74
HpaI GTT'AAC
Cuts at: 0 459 4668
Size: 459 4209
MfeI C'AATT_G
Cuts at: 0 551 4668
Size: 551 4117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2630 3325 4668
Size: 2630 695 1343
Fragments arranged by size:
2630 1343 695
MscI TGG'CCA
Cuts at: 0 1590 3857 4668
Size: 1590 2267 811
Fragments arranged by size:
2267 1590 811
MslI CAynn'nnrTG
Cuts at: 0 110 434 1810 4016 4668
Size: 110 324 1376 2206 652
Fragments arranged by size:
2206 1376 652 324 110
MspA1I CmG'CkG
Cuts at: 0 6 46 1582 1990 2643 4352 4668
Size: 6 40 1536 408 653 1709 316
Fragments arranged by size:
1709 1536 653 408 316 40 6
NdeI CA'TA_TG
Cuts at: 0 330 2889 4668
Size: 330 2559 1779
Fragments arranged by size:
2559 1779 330
NheI G'CTAG_C
Cuts at: 0 2796 4668
Size: 2796 1872
NruI TCG'CGA
Cuts at: 0 2420 4668
Size: 2420 2248
NsiI A_TGCA'T
Cuts at: 0 1311 2889 4668
Size: 1311 1578 1779
Fragments arranged by size:
1779 1578 1311
NspI r_CATG'y
Cuts at: 0 457 2430 2508 4668
Size: 457 1973 78 2160
Fragments arranged by size:
2160 1973 457 78
PciI A'CATG_T
Cuts at: 0 453 4668
Size: 453 4215
PflMI CCAn_nnn'nTGG
Cuts at: 0 706 4668
Size: 706 3962
PfoI T'CCnGG_A
Cuts at: 0 1943 2460 4668
Size: 1943 517 2208
Fragments arranged by size:
2208 1943 517
PmlI CAC'GTG
Cuts at: 0 2626 4668
Size: 2626 2042
PpuMI rG'GwC_Cy
Cuts at: 0 4386 4668
Size: 4386 282
PshAI GACnn'nnGTC
Cuts at: 0 2618 4668
Size: 2618 2050
PsiI TTA'TAA
Cuts at: 0 3326 4668
Size: 3326 1342
PstI C_TGCA'G
Cuts at: 0 1566 1612 4668
Size: 1566 46 3056
Fragments arranged by size:
3056 1566 46
PvuII CAG'CTG
Cuts at: 0 1582 1990 2643 4352 4668
Size: 1582 408 653 1709 316
Fragments arranged by size:
1709 1582 653 408 316
SacI G_AGCT'C
Cuts at: 0 2366 4668
Size: 2366 2302
SacII CC_GC'GG
Cuts at: 0 47 4668
Size: 47 4621
SalI G'TCGA_C
Cuts at: 0 3584 4668
Size: 3584 1084
SapI GCTCTTCn'nnn_
Cuts at: 0 3843 4668
Size: 3843 825
ScaI AGT'ACT
Cuts at: 0 3183 4668
Size: 3183 1485
SfcI C'TryA_G
Cuts at: 0 1562 1608 1742 3968 4668
Size: 1562 46 134 2226 700
Fragments arranged by size:
2226 1562 700 134 46
SmlI C'TyrA_G
Cuts at: 0 202 1219 2023 2549 3544 3755 3846 4668
Size: 202 1017 804 526 995 211 91 822
Fragments arranged by size:
1017 995 822 804 526 211 202 91
SspI AAT'ATT
Cuts at: 0 4176 4668
Size: 4176 492
StyI C'CwwG_G
Cuts at: 0 2411 2616 2973 3404 3632 4668
Size: 2411 205 357 431 228 1036
Fragments arranged by size:
2411 1036 431 357 228 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 807 3921 4629 4668
Size: 807 3114 708 39
Fragments arranged by size:
3114 807 708 39
TatI w'GTAC_w
Cuts at: 0 2541 3181 3202 4668
Size: 2541 640 21 1466
Fragments arranged by size:
2541 1466 640 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 369 401 4668
Size: 369 32 4267
Fragments arranged by size:
4267 369 32
XbaI T'CTAG_A
Cuts at: 0 2728 3601 3616 4668
Size: 2728 873 15 1052
Fragments arranged by size:
2728 1052 873 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1858 4668
Size: 1858 2810
XmnI GAAnn'nnTTC
Cuts at: 0 2877 4272 4668
Size: 2877 1395 396
Fragments arranged by size:
2877 1395 396
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AhdI AleI AlwNI
ApaLI ApoI BaeI BanII BbsI BbvCI BciVI BfrBI
BglII Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaXI BseRI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NdeI NheI
NruI NsiI NspI PciI PflMI PfoI PmlI PpuMI
PshAI PsiI PstI PvuII SacI SacII SalI SapI
ScaI SfcI SmlI SspI StyI TaqII TatI TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AlfI AloI ApaI
AscI AseI AsiSI AvaI AvrII BamHI BanI BbeI
BcgI BclI BglI BlpI BsaBI BsaHI BsaWI BseYI
BsiWI BsmBI BspEI BssHII BstEII BstZ17I CspCI DrdI
EagI EcoNI FseI FspI FspAI HaeII KasI KpnI
MluI NaeI NarI NcoI NgoMIV NotI PacI PasI
PmeI PpiI PspOMI PspXI PsrI PvuI RsrII SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XhoI XmaI
ZraI