Restriction map for
pF1KB4088
(Linear) (Six-base) MAPSORT of: KIBB4088.seq Check: 3157 from: 1 to: 2280
KIBB4088.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AccI GT'mk_AC
Cuts at: 0 621 1507 2280
Size: 621 886 773
Fragments arranged by size:
886 773 621
Acc65I G'GTAC_C
Cuts at: 0 1468 1699 2280
Size: 1468 231 581
Fragments arranged by size:
1468 581 231
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 2280
Size: 400 1880
AfeI AGC'GCT
Cuts at: 0 585 2280
Size: 585 1695
AflIII A'CryG_T
Cuts at: 0 1084 2094 2280
Size: 1084 1010 186
Fragments arranged by size:
1084 1010 186
AleI CACnn'nnGTG
Cuts at: 0 745 2280
Size: 745 1535
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 300 332 2280
Size: 300 32 1948
Fragments arranged by size:
1948 300 32
AlwNI CAG_nnn'CTG
Cuts at: 0 685 1580 1923 2280
Size: 685 895 343 357
Fragments arranged by size:
895 685 357 343
ApaI G_GGCC'C
Cuts at: 0 1221 1240 2280
Size: 1221 19 1040
Fragments arranged by size:
1221 1040 19
ApoI r'AATT_y
Cuts at: 0 447 523 859 1154 2280
Size: 447 76 336 295 1126
Fragments arranged by size:
1126 447 336 295 76
AseI AT'TA_AT
Cuts at: 0 1445 2280
Size: 1445 835
BanI G'GyrC_C
Cuts at: 0 424 1384 1468 1699 2280
Size: 424 960 84 231 581
Fragments arranged by size:
960 581 424 231 84
BanII G_rGCy'C
Cuts at: 0 1221 1240 2110 2280
Size: 1221 19 870 170
Fragments arranged by size:
1221 870 170 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 2091 2280
Size: 2091 189
BciVI GTATCCnnnnn_n'
Cuts at: 0 1399 2280
Size: 1399 881
BclI T'GATC_A
Cuts at: 0 549 599 2280
Size: 549 50 1681
Fragments arranged by size:
1681 549 50
BfrBI ATG'CAT
Cuts at: 0 1231 2280
Size: 1231 1049
Bme1580I G_kGCm'C
Cuts at: 0 1221 1240 2280
Size: 1221 19 1040
Fragments arranged by size:
1221 1040 19
BmgBI CAC'GTC
Cuts at: 0 358 2280
Size: 358 1922
BmrI ACTGGGnnnn_n'
Cuts at: 0 1562 1590 2280
Size: 1562 28 690
Fragments arranged by size:
1562 690 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 996 1262 1385 2158 2280
Size: 302 694 266 123 773 122
Fragments arranged by size:
773 694 302 266 123 122
BsaAI yAC'GTr
Cuts at: 0 2067 2280
Size: 2067 213
BsaBI GATnn'nnATC
Cuts at: 0 488 2280
Size: 488 1792
BsaWI w'CCGG_w
Cuts at: 0 1255 1545 2280
Size: 1255 290 735
Fragments arranged by size:
1255 735 290
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 300 330 2280
Size: 300 30 1950
Fragments arranged by size:
1950 300 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 343 2280
Size: 343 1937
BseYI C'CCAG_C
Cuts at: 0 1330 1650 2280
Size: 1330 320 630
Fragments arranged by size:
1330 630 320
BsmI GAATG_Cn'
Cuts at: 0 22 1989 2280
Size: 22 1967 291
Fragments arranged by size:
1967 291 22
Bsp1286I G_dGCh'C
Cuts at: 0 1221 1240 2110 2280
Size: 1221 19 870 170
Fragments arranged by size:
1221 870 170 19
BspHI T'CATG_A
Cuts at: 0 2009 2033 2280
Size: 2009 24 247
Fragments arranged by size:
2009 247 24
BsrDI GCAATG_nn'
Cuts at: 0 1140 2280
Size: 1140 1140
BsrFI r'CCGG_y
Cuts at: 0 421 2280
Size: 421 1859
BssSI C'ACGA_G
Cuts at: 0 461 1760 1934 2280
Size: 461 1299 174 346
Fragments arranged by size:
1299 461 346 174
BstEII G'GTnAC_C
Cuts at: 0 916 1095 2280
Size: 916 179 1185
Fragments arranged by size:
1185 916 179
BstYI r'GATC_y
Cuts at: 0 472 754 1869 2280
Size: 472 282 1115 411
Fragments arranged by size:
1115 472 411 282
BstZ17I GTA'TAC
Cuts at: 0 1508 2280
Size: 1508 772
BtgI C'CryG_G
Cuts at: 0 1706 2280
Size: 1706 574
DraI TTT'AAA
Cuts at: 0 528 1427 2280
Size: 528 899 853
Fragments arranged by size:
899 853 528
DrdI GACnn_nn'nnGTC
Cuts at: 0 2005 2280
Size: 2005 275
EarI CTCTTCn'nnn_
Cuts at: 0 376 2280
Size: 376 1904
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 400 996 1262 1385 2158 2280
Size: 302 98 596 266 123 773 122
Fragments arranged by size:
773 596 302 266 123 122 98
EcoO109I rG'GnC_Cy
Cuts at: 0 1217 1236 1237 2280
Size: 1217 19 1 1043
Fragments arranged by size:
1217 1043 19 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1480 2280
Size: 1480 800
HaeII r_GCGC'y
Cuts at: 0 587 2280
Size: 587 1693
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 457 489 1045 1077 1360 1392 2280
Size: 457 32 556 32 283 32 888
Fragments arranged by size:
888 556 457 283 32 32 32
HincII GTy'rAC
Cuts at: 0 873 2280
Size: 873 1407
HindIII A'AGCT_T
Cuts at: 0 816 2280
Size: 816 1464
HpaI GTT'AAC
Cuts at: 0 873 2280
Size: 873 1407
KpnI G_GTAC'C
Cuts at: 0 1472 1703 2280
Size: 1472 231 577
Fragments arranged by size:
1472 577 231
MfeI C'AATT_G
Cuts at: 0 827 2280
Size: 827 1453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1336 2115 2280
Size: 374 962 779 165
Fragments arranged by size:
962 779 374 165
MslI CAynn'nnrTG
Cuts at: 0 745 2038 2280
Size: 745 1293 242
Fragments arranged by size:
1293 745 242
MspA1I CmG'CkG
Cuts at: 0 1768 2280
Size: 1768 512
NcoI C'CATG_G
Cuts at: 0 1706 2280
Size: 1706 574
NdeI CA'TA_TG
Cuts at: 0 453 2280
Size: 453 1827
NsiI A_TGCA'T
Cuts at: 0 1233 2280
Size: 1233 1047
NspI r_CATG'y
Cuts at: 0 901 1088 2098 2280
Size: 901 187 1010 182
Fragments arranged by size:
1010 901 187 182
PciI A'CATG_T
Cuts at: 0 1084 2094 2280
Size: 1084 1010 186
Fragments arranged by size:
1084 1010 186
PflMI CCAn_nnn'nTGG
Cuts at: 0 1394 2280
Size: 1394 886
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 300 332 2280
Size: 300 32 1948
Fragments arranged by size:
1948 300 32
PspOMI G'GGCC_C
Cuts at: 0 1217 1236 2280
Size: 1217 19 1044
Fragments arranged by size:
1217 1044 19
PstI C_TGCA'G
Cuts at: 0 354 1026 1773 2280
Size: 354 672 747 507
Fragments arranged by size:
747 672 507 354
PvuII CAG'CTG
Cuts at: 0 1768 2280
Size: 1768 512
SexAI A'CCwGG_T
Cuts at: 0 632 2280
Size: 632 1648
SfcI C'TryA_G
Cuts at: 0 122 350 758 1022 1769 2280
Size: 122 228 408 264 747 511
Fragments arranged by size:
747 511 408 264 228 122
SnaBI TAC'GTA
Cuts at: 0 2067 2280
Size: 2067 213
StyI C'CwwG_G
Cuts at: 0 1706 2280
Size: 1706 574
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 962 2280
Size: 962 1318
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 746 778 920 945 952 977 1671 1703
Size: 746 32 142 25 7 25 694 32
Cuts at: 1703 2280
Size: 577
Fragments arranged by size:
746 694 577 142 32 32 25 25
7
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 706 2280
Size: 706 1574
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AleI AloI AlwNI
ApaI ApoI AseI BanI BanII BbsI BciVI BclI
BfrBI Bme1580I BmgBI BmrI BpmI BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsmI Bsp1286I BspHI BsrDI BsrFI
BssSI BstEII BstYI BstZ17I BtgI DraI DrdI EarI
Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MmeI MslI MspA1I NcoI NdeI NsiI
NspI PciI PflMI PpiI PspOMI PstI PvuII SexAI
SfcI SnaBI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AlfI ApaLI
AscI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI
BcgI BglI BglII BlpI BmtI BplI Bpu10I BpuEI
BsaI BsaHI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspMI BsrBI BsrGI BssHII BstAPI BstBI BstXI Bsu36I
BtgZI BtsI ClaI CspCI DraIII EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI KasI
MluI MscI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PfoI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XhoI XmaI XmnI ZraI