Restriction map for
pF1KB4080
(Linear) (Six-base) MAPSORT of: KIBB4080.seq Check: 4588 from: 1 to: 1014
KIBB4080.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 0 675 1014
Size: 675 339
Acc65I G'GTAC_C
Cuts at: 0 613 1014
Size: 613 401
AfeI AGC'GCT
Cuts at: 0 304 1014
Size: 304 710
AflIII A'CryG_T
Cuts at: 0 83 137 1014
Size: 83 54 877
Fragments arranged by size:
877 83 54
ApoI r'AATT_y
Cuts at: 0 241 503 512 625 776 915 1014
Size: 241 262 9 113 151 139 99
Fragments arranged by size:
262 241 151 139 113 99 9
AseI AT'TA_AT
Cuts at: 0 590 1014
Size: 590 424
AvaI C'yCGr_G
Cuts at: 0 839 1014
Size: 839 175
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 389 422 1014
Size: 389 33 592
Fragments arranged by size:
592 389 33
BanI G'GyrC_C
Cuts at: 0 613 1014
Size: 613 401
BanII G_rGCy'C
Cuts at: 0 321 1014
Size: 321 693
BbsI GAAGACnn'nnnn_
Cuts at: 0 481 1014
Size: 481 533
BciVI GTATCCnnnnn_n'
Cuts at: 0 144 1014
Size: 144 870
BglII A'GATC_T
Cuts at: 0 956 1014
Size: 956 58
BlpI GC'TnA_GC
Cuts at: 0 557 1014
Size: 557 457
BmgBI CAC'GTC
Cuts at: 0 86 1014
Size: 86 928
BmrI ACTGGGnnnn_n'
Cuts at: 0 90 393 1014
Size: 90 303 621
Fragments arranged by size:
621 303 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 547 1014
Size: 386 161 467
Fragments arranged by size:
467 386 161
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 856 886 1014
Size: 856 30 128
Fragments arranged by size:
856 128 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 1014
Size: 412 602
BsmI GAATG_Cn'
Cuts at: 0 644 1014
Size: 644 370
Bsp1286I G_dGCh'C
Cuts at: 0 321 1014
Size: 321 693
BstXI CCAn_nnnn'nTGG
Cuts at: 0 383 781 1014
Size: 383 398 233
Fragments arranged by size:
398 383 233
BstYI r'GATC_y
Cuts at: 0 956 1014
Size: 956 58
BstZ17I GTA'TAC
Cuts at: 0 676 1014
Size: 676 338
BtsI GCAGTG_nn'
Cuts at: 0 183 627 1014
Size: 183 444 387
Fragments arranged by size:
444 387 183
EcoRI G'AATT_C
Cuts at: 0 503 512 1014
Size: 503 9 502
Fragments arranged by size:
503 502 9
EcoRV GAT'ATC
Cuts at: 0 186 1014
Size: 186 828
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 276 321 1014
Size: 276 45 693
Fragments arranged by size:
693 276 45
HaeII r_GCGC'y
Cuts at: 0 306 1014
Size: 306 708
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 468 500 1014
Size: 468 32 514
Fragments arranged by size:
514 468 32
HindIII A'AGCT_T
Cuts at: 0 195 1014
Size: 195 819
KpnI G_GTAC'C
Cuts at: 0 617 1014
Size: 617 397
MslI CAynn'nnrTG
Cuts at: 0 370 1014
Size: 370 644
MspA1I CmG'CkG
Cuts at: 0 801 929 1014
Size: 801 128 85
Fragments arranged by size:
801 128 85
NspI r_CATG'y
Cuts at: 0 141 885 1014
Size: 141 744 129
Fragments arranged by size:
744 141 129
PciI A'CATG_T
Cuts at: 0 137 1014
Size: 137 877
PspXI vC'TCGA_Gb
Cuts at: 0 839 1014
Size: 839 175
PvuII CAG'CTG
Cuts at: 0 929 1014
Size: 929 85
ScaI AGT'ACT
Cuts at: 0 997 1014
Size: 997 17
SmlI C'TyrA_G
Cuts at: 0 401 562 839 1014
Size: 401 161 277 175
Fragments arranged by size:
401 277 175 161
TatI w'GTAC_w
Cuts at: 0 995 1014
Size: 995 19
XhoI C'TCGA_G
Cuts at: 0 839 1014
Size: 839 175
XmnI GAAnn'nnTTC
Cuts at: 0 769 1014
Size: 769 245
Enzymes that do cut:
AccI Acc65I AfeI AflIII ApoI AseI AvaI BaeI
BanI BanII BbsI BciVI BglII BlpI BmgBI BmrI
BpuEI BsaXI BsgI BsmI Bsp1286I BstXI BstYI BstZ17I
BtsI EcoRI EcoRV FalI HaeII Hin4I HindIII KpnI
MslI MspA1I NspI PciI PspXI PvuII ScaI SmlI
TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII
BamHI BbeI BbvCI BcgI BclI BfrBI BglI Bme1580I
BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI FspI FspAI HincII HpaI
KasI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TstI Tth111I XbaI XcmI XmaI
ZraI