(Linear) (Six-base) MAPSORT of: KIBB4062.seq  Check: 930  from: 1  to: 525



KIBB4062.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     245     525 
   Size:        245     280
 
AlwNI CAG_nnn'CTG

Cuts at:      0     306     375     443     525 
   Size:        306      69      68      82

  Fragments arranged by size: 

                 306      82      69      68
 
BamHI G'GATC_C

Cuts at:      0      44     525 
   Size:         44     481
 
BanII G_rGCy'C

Cuts at:      0     497     525 
   Size:        497      28
 
BglI GCCn_nnn'nGGC

Cuts at:      0     219     525 
   Size:        219     306
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     245     424     525 
   Size:        245     179     101

  Fragments arranged by size: 

                 245     179     101
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     253     525 
   Size:        253     272
 
BsaHI Gr'CG_yC

Cuts at:      0     341     525 
   Size:        341     184
 
BseYI C'CCAG_C

Cuts at:      0     102     525 
   Size:        102     423
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     472     525 
   Size:        292     180      53

  Fragments arranged by size: 

                 292     180      53
 
BsiEI CG_ry'CG

Cuts at:      0      71     214     276     282     525 
   Size:         71     143      62       6     243

  Fragments arranged by size: 

                 243     143      71      62       6
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     142     525 
   Size:        142     383
 
Bsp1286I G_dGCh'C

Cuts at:      0     497     525 
   Size:        497      28
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     383     525 
   Size:        383     142
 
BsrBI CCG'CTC

Cuts at:      0     216     525 
   Size:        216     309
 
BsrFI r'CCGG_y

Cuts at:      0      70     525 
   Size:         70     455
 
BsrGI T'GTAC_A

Cuts at:      0      89     525 
   Size:         89     436
 
BssSI C'ACGA_G

Cuts at:      0     397     525 
   Size:        397     128
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     375     525 
   Size:        375     150
 
BstEII G'GTnAC_C

Cuts at:      0     114     525 
   Size:        114     411
 
BstYI r'GATC_y

Cuts at:      0      44     206     401     525 
   Size:         44     162     195     124

  Fragments arranged by size: 

                 195     162     124      44
 
Bsu36I CC'TnA_GG

Cuts at:      0     471     525 
   Size:        471      54
 
ClaI AT'CG_AT

Cuts at:      0     287     525 
   Size:        287     238
 
EaeI y'GGCC_r

Cuts at:      0      68     195     211     525 
   Size:         68     127      16     314

  Fragments arranged by size: 

                 314     127      68      16
 
EagI C'GGCC_G

Cuts at:      0      68     211     525 
   Size:         68     143     314

  Fragments arranged by size: 

                 314     143      68
 
EcoO109I rG'GnC_Cy

Cuts at:      0     239     372     525 
   Size:        239     133     153

  Fragments arranged by size: 

                 239     153     133
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     231     263     525 
   Size:        231      32     262

  Fragments arranged by size: 

                 262     231      32
 
HincII GTy'rAC

Cuts at:      0     506     525 
   Size:        506      19
 
HpaI GTT'AAC

Cuts at:      0     506     525 
   Size:        506      19
 
NaeI GCC'GGC

Cuts at:      0      72     525 
   Size:         72     453
 
NgoMIV G'CCGG_C

Cuts at:      0      70     525 
   Size:         70     455
 
PfoI T'CCnGG_A

Cuts at:      0     404     525 
   Size:        404     121
 
PpuMI rG'GwC_Cy

Cuts at:      0     239     372     525 
   Size:        239     133     153

  Fragments arranged by size: 

                 239     153     133
 
SanDI GG'GwC_CC

Cuts at:      0     239     525 
   Size:        239     286
 
StyI C'CwwG_G

Cuts at:      0     243     525 
   Size:        243     282
 
TatI w'GTAC_w

Cuts at:      0      89     525 
   Size:         89     436
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     129     525 
   Size:        129     396
 

 Enzymes that do cut:

     AhdI    AlwNI    BamHI    BanII     BglI     BmrI     BsaI    BsaHI
    BseYI     BsgI    BsiEI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI
    BsrGI    BssSI   BstAPI   BstEII    BstYI   Bsu36I     ClaI     EaeI
     EagI EcoO109I    Hin4I   HincII     HpaI     NaeI   NgoMIV     PfoI
    PpuMI    SanDI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI  BsiHKAI    BsiWI     BsmI    BspEI
    BspHI    BsrDI   BssHII    BstBI    BstXI  BstZ17I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI