Restriction map for
pF1KB4057
(Linear) (Six-base) MAPSORT of: KIBB4057.seq Check: 9000 from: 1 to: 2061
KIBB4057.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 0 1472 2061
Size: 1472 589
AccI GT'mk_AC
Cuts at: 0 161 653 1205 2061
Size: 161 492 552 856
Fragments arranged by size:
856 552 492 161
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 631 1291 2038 2061
Size: 159 472 660 747 23
Fragments arranged by size:
747 660 472 159 23
AhdI GACnn_n'nnGTC
Cuts at: 0 1227 2061
Size: 1227 834
AleI CACnn'nnGTG
Cuts at: 0 274 2061
Size: 274 1787
AlwNI CAG_nnn'CTG
Cuts at: 0 1068 2061
Size: 1068 993
ApaI G_GGCC'C
Cuts at: 0 1565 1695 2061
Size: 1565 130 366
Fragments arranged by size:
1565 366 130
ApaLI G'TGCA_C
Cuts at: 0 1234 2061
Size: 1234 827
ApoI r'AATT_y
Cuts at: 0 540 2061
Size: 540 1521
AvaI C'yCGr_G
Cuts at: 0 1564 2061
Size: 1564 497
BamHI G'GATC_C
Cuts at: 0 379 563 1211 1427 1775 2061
Size: 379 184 648 216 348 286
Fragments arranged by size:
648 379 348 286 216 184
BanI G'GyrC_C
Cuts at: 0 425 866 1573 2061
Size: 425 441 707 488
Fragments arranged by size:
707 488 441 425
BanII G_rGCy'C
Cuts at: 0 511 1565 1695 1790 2061
Size: 511 1054 130 95 271
Fragments arranged by size:
1054 511 271 130 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 828 1906 2061
Size: 828 1078 155
Fragments arranged by size:
1078 828 155
BbvCI CC'TCA_GC
Cuts at: 0 349 862 1499 1814 2061
Size: 349 513 637 315 247
Fragments arranged by size:
637 513 349 315 247
BglI GCCn_nnn'nGGC
Cuts at: 0 117 847 2061
Size: 117 730 1214
Fragments arranged by size:
1214 730 117
Bme1580I G_kGCm'C
Cuts at: 0 430 1238 1533 1565 1695 2061
Size: 430 808 295 32 130 366
Fragments arranged by size:
808 430 366 295 130 32
BmgBI CAC'GTC
Cuts at: 0 1200 2061
Size: 1200 861
BmrI ACTGGGnnnn_n'
Cuts at: 0 1111 2061
Size: 1111 950
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1362 1626 1643 2061
Size: 1362 264 17 418
Fragments arranged by size:
1362 418 264 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 61 376 1621 1771 2061
Size: 55 6 315 1245 150 290
Fragments arranged by size:
1245 315 290 150 55 6
Bpu10I CC'TnA_GC
Cuts at: 0 349 862 1150 1499 1814 2061
Size: 349 513 288 349 315 247
Fragments arranged by size:
513 349 349 315 288 247
BsaI GGTCTCn'nnnn_
Cuts at: 0 37 50 2061
Size: 37 13 2011
Fragments arranged by size:
2011 37 13
BsaAI yAC'GTr
Cuts at: 0 657 2061
Size: 657 1404
BsaBI GATnn'nnATC
Cuts at: 0 1774 2061
Size: 1774 287
BsaHI Gr'CG_yC
Cuts at: 0 743 1469 2061
Size: 743 726 592
Fragments arranged by size:
743 726 592
BsaWI w'CCGG_w
Cuts at: 0 1772 2036 2061
Size: 1772 264 25
Fragments arranged by size:
1772 264 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 265 475 1366 1420 1516 1687 1906
Size: 22 243 210 891 54 96 171 219
Cuts at: 1906 2061
Size: 155
Fragments arranged by size:
891 243 219 210 171 155 96 54
22
BseYI C'CCAG_C
Cuts at: 0 192 393 429 485 711 719 830 1007
Size: 192 201 36 56 226 8 111 177
Cuts at: 1007 2061
Size: 1054
Fragments arranged by size:
1054 226 201 192 177 111 56 36
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 276 837 1842 2061
Size: 112 164 561 1005 219
Fragments arranged by size:
1005 561 219 164 112
BsiEI CG_ry'CG
Cuts at: 0 71 624 2061
Size: 71 553 1437
Fragments arranged by size:
1437 553 71
BsiHKAI G_wGCw'C
Cuts at: 0 1156 1238 2061
Size: 1156 82 823
Fragments arranged by size:
1156 823 82
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1292 2061
Size: 1292 769
Bsp1286I G_dGCh'C
Cuts at: 0 430 511 1156 1238 1533 1565 1695 1790
Size: 430 81 645 82 295 32 130 95
Cuts at: 1790 2061
Size: 271
Fragments arranged by size:
645 430 295 271 130 95 82 81
32
BspEI T'CCGG_A
Cuts at: 0 1772 2036 2061
Size: 1772 264 25
Fragments arranged by size:
1772 264 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1046 1592 1733 1967 2061
Size: 1046 546 141 234 94
Fragments arranged by size:
1046 546 234 141 94
BsrBI CCG'CTC
Cuts at: 0 465 1305 1836 1968 2061
Size: 465 840 531 132 93
Fragments arranged by size:
840 531 465 132 93
BsrDI GCAATG_nn'
Cuts at: 0 957 2061
Size: 957 1104
BsrFI r'CCGG_y
Cuts at: 0 187 619 1879 2061
Size: 187 432 1260 182
Fragments arranged by size:
1260 432 187 182
BssSI C'ACGA_G
Cuts at: 0 1699 2061
Size: 1699 362
BstXI CCAn_nnnn'nTGG
Cuts at: 0 276 2061
Size: 276 1785
BstYI r'GATC_y
Cuts at: 0 379 563 965 1211 1427 1553 1769 1775
Size: 379 184 402 246 216 126 216 6
Cuts at: 1775 1910 2061
Size: 135 151
Fragments arranged by size:
402 379 246 216 216 184 151 135
126 6
BtgI C'CryG_G
Cuts at: 0 272 798 845 1922 2061
Size: 272 526 47 1077 139
Fragments arranged by size:
1077 526 272 139 47
BtsI GCAGTG_nn'
Cuts at: 0 1002 1464 2061
Size: 1002 462 597
Fragments arranged by size:
1002 597 462
DrdI GACnn_nn'nnGTC
Cuts at: 0 1468 2061
Size: 1468 593
EaeI y'GGCC_r
Cuts at: 0 2 51 68 320 512 621 822 1394
Size: 2 49 17 252 192 109 201 572
Cuts at: 1394 1418 2061
Size: 24 643
Fragments arranged by size:
643 572 252 201 192 109 49 24
17 2
EagI C'GGCC_G
Cuts at: 0 68 621 2061
Size: 68 553 1440
Fragments arranged by size:
1440 553 68
EarI CTCTTCn'nnn_
Cuts at: 0 418 452 1084 1612 2061
Size: 418 34 632 528 449
Fragments arranged by size:
632 528 449 418 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 61 159 376 631 1291 1621 1771
Size: 55 6 98 217 255 660 330 150
Cuts at: 1771 2038 2061
Size: 267 23
Fragments arranged by size:
660 330 267 255 217 150 98 55
23 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1368 1681 1888 2061
Size: 1368 313 207 173
Fragments arranged by size:
1368 313 207 173
EcoO109I rG'GnC_Cy
Cuts at: 0 1065 1343 1561 1692 1740 2061
Size: 1065 278 218 131 48 321
Fragments arranged by size:
1065 321 278 218 131 48
EcoRV GAT'ATC
Cuts at: 0 1314 2061
Size: 1314 747
FseI GG_CCGG'CC
Cuts at: 0 1883 2061
Size: 1883 178
HaeII r_GCGC'y
Cuts at: 0 789 2061
Size: 789 1272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 15 47 1213 1245 2061
Size: 15 32 1166 32 816
Fragments arranged by size:
1166 816 32 32 15
HincII GTy'rAC
Cuts at: 0 597 684 2061
Size: 597 87 1377
Fragments arranged by size:
1377 597 87
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1697 2061
Size: 1697 364
MscI TGG'CCA
Cuts at: 0 824 1420 2061
Size: 824 596 641
Fragments arranged by size:
824 641 596
MslI CAynn'nnrTG
Cuts at: 0 274 1450 1684 2061
Size: 274 1176 234 377
Fragments arranged by size:
1176 377 274 234
MspA1I CmG'CkG
Cuts at: 0 433 1498 2061
Size: 433 1065 563
Fragments arranged by size:
1065 563 433
NaeI GCC'GGC
Cuts at: 0 621 1881 2061
Size: 621 1260 180
Fragments arranged by size:
1260 621 180
NgoMIV G'CCGG_C
Cuts at: 0 619 1879 2061
Size: 619 1260 182
Fragments arranged by size:
1260 619 182
PflMI CCAn_nnn'nTGG
Cuts at: 0 758 1989 2061
Size: 758 1231 72
Fragments arranged by size:
1231 758 72
PfoI T'CCnGG_A
Cuts at: 0 757 2061
Size: 757 1304
PpuMI rG'GwC_Cy
Cuts at: 0 1343 1740 2061
Size: 1343 397 321
Fragments arranged by size:
1343 397 321
PspOMI G'GGCC_C
Cuts at: 0 1561 1691 2061
Size: 1561 130 370
Fragments arranged by size:
1561 370 130
PstI C_TGCA'G
Cuts at: 0 308 1043 1829 2061
Size: 308 735 786 232
Fragments arranged by size:
786 735 308 232
SapI GCTCTTCn'nnn_
Cuts at: 0 418 1084 1612 2061
Size: 418 666 528 449
Fragments arranged by size:
666 528 449 418
ScaI AGT'ACT
Cuts at: 0 943 2061
Size: 943 1118
SfcI C'TryA_G
Cuts at: 0 304 1039 1825 2061
Size: 304 735 786 236
Fragments arranged by size:
786 735 304 236
StuI AGG'CCT
Cuts at: 0 361 1363 2061
Size: 361 1002 698
Fragments arranged by size:
1002 698 361
StyI C'CwwG_G
Cuts at: 0 218 535 1779 2061
Size: 218 317 1244 282
Fragments arranged by size:
1244 317 282 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 499 999 1333 2061
Size: 499 500 334 728
Fragments arranged by size:
728 500 499 334
TatI w'GTAC_w
Cuts at: 0 941 2061
Size: 941 1120
Tth111I GACn'n_nGTC
Cuts at: 0 742 2061
Size: 742 1319
XbaI T'CTAG_A
Cuts at: 0 588 2061
Size: 588 1473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 390 1222 2061
Size: 390 832 839
Fragments arranged by size:
839 832 390
XmnI GAAnn'nnTTC
Cuts at: 0 2032 2061
Size: 2032 29
ZraI GAC'GTC
Cuts at: 0 1470 2061
Size: 1470 591
Enzymes that do cut:
AatII AccI AcuI AhdI AleI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI
BstXI BstYI BtgI BtsI DrdI EaeI EagI EarI
Eco57MI EcoNI EcoO109I EcoRV FseI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NaeI NgoMIV PflMI PfoI
PpuMI PspOMI PstI SapI ScaI SfcI StuI StyI
TaqII TatI Tth111I XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AlfI
AloI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BciVI BclI BfrBI BglII BlpI BmtI BpuEI BsaXI
BsiWI BsmI BspHI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EciI
EcoICRI EcoRI FalI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TstI XhoI XmaI