(Linear) (Six-base) MAPSORT of: KIBB4054.seq  Check: 1385  from: 1  to: 414



KIBB4054.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AccI GT'mk_AC

Cuts at:      0      48     414 
   Size:         48     366
 
ApaI G_GGCC'C

Cuts at:      0      96     414 
   Size:         96     318
 
BanII G_rGCy'C

Cuts at:      0      96     133     414 
   Size:         96      37     281

  Fragments arranged by size: 

                 281      96      37
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     353     414 
   Size:        353      61
 
BfrBI ATG'CAT

Cuts at:      0     411     414 
   Size:        411       3
 
Bme1580I G_kGCm'C

Cuts at:      0      96     414 
   Size:         96     318
 
BsaBI GATnn'nnATC

Cuts at:      0     259     414 
   Size:        259     155
 
BseYI C'CCAG_C

Cuts at:      0      77     132     414 
   Size:         77      55     282

  Fragments arranged by size: 

                 282      77      55
 
BsmI GAATG_Cn'

Cuts at:      0     413     414 
   Size:        413       1
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     281     414 
   Size:        281     133
 
Bsp1286I G_dGCh'C

Cuts at:      0      96     133     414 
   Size:         96      37     281

  Fragments arranged by size: 

                 281      96      37
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     243     414 
   Size:        243     171
 
BsrDI GCAATG_nn'

Cuts at:      0     215     414 
   Size:        215     199
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     257     414 
   Size:        257     157
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     382     414 
   Size:        382      32
 
EcoO109I rG'GnC_Cy

Cuts at:      0      92      93     414 
   Size:         92       1     321

  Fragments arranged by size: 

                 321      92       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     194     414 
   Size:        194     220
 
MfeI C'AATT_G

Cuts at:      0      52     414 
   Size:         52     362
 
NsiI A_TGCA'T

Cuts at:      0     413     414 
   Size:        413       1
 
PspOMI G'GGCC_C

Cuts at:      0      92     414 
   Size:         92     322
 
PstI C_TGCA'G

Cuts at:      0     240     414 
   Size:        240     174
 
SfcI C'TryA_G

Cuts at:      0     106     236     414 
   Size:        106     130     178

  Fragments arranged by size: 

                 178     130     106
 
StuI AGG'CCT

Cuts at:      0      72     187     414 
   Size:         72     115     227

  Fragments arranged by size: 

                 227     115      72
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      53     153     368     414 
   Size:         53     100     215      46

  Fragments arranged by size: 

                 215     100      53      46
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      85     414 
   Size:         85     329
 

 Enzymes that do cut:

     AccI     ApaI    BanII     BbsI    BfrBI Bme1580I    BsaBI    BseYI
     BsmI    BsmBI Bsp1286I    BspMI    BsrDI    BstXI     EciI EcoO109I
     FalI     MfeI     NsiI   PspOMI     PstI     SfcI     StuI    TaqII
     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI
    BsiWI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI