Restriction map for
pF1KB4038
(Linear) (Six-base) MAPSORT of: KIBB4038.seq Check: 1663 from: 1 to: 1089
KIBB4038.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 0 629 1089
Size: 629 460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 30 1089
Size: 30 1059
AlwNI CAG_nnn'CTG
Cuts at: 0 882 1089
Size: 882 207
ApaI G_GGCC'C
Cuts at: 0 245 1089
Size: 245 844
ApoI r'AATT_y
Cuts at: 0 204 1089
Size: 204 885
BanI G'GyrC_C
Cuts at: 0 892 1089
Size: 892 197
BanII G_rGCy'C
Cuts at: 0 156 245 302 1089
Size: 156 89 57 787
Fragments arranged by size:
787 156 89 57
BbsI GAAGACnn'nnnn_
Cuts at: 0 905 1071 1089
Size: 905 166 18
Fragments arranged by size:
905 166 18
BbvCI CC'TCA_GC
Cuts at: 0 659 1089
Size: 659 430
BglII A'GATC_T
Cuts at: 0 779 950 1089
Size: 779 171 139
Fragments arranged by size:
779 171 139
Bme1580I G_kGCm'C
Cuts at: 0 245 1089
Size: 245 844
BmrI ACTGGGnnnn_n'
Cuts at: 0 666 1089
Size: 666 423
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 316 1089
Size: 316 773
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 655 760 859 1006 1089
Size: 483 172 105 99 147 83
Fragments arranged by size:
483 172 147 105 99 83
Bpu10I CC'TnA_GC
Cuts at: 0 467 659 1089
Size: 467 192 430
Fragments arranged by size:
467 430 192
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 1089
Size: 345 744
BsaI GGTCTCn'nnnn_
Cuts at: 0 661 1089
Size: 661 428
BsaWI w'CCGG_w
Cuts at: 0 1082 1089
Size: 1082 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 512 542 928 958 1089
Size: 512 30 386 30 131
Fragments arranged by size:
512 386 131 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 310 949 1089
Size: 61 249 639 140
Fragments arranged by size:
639 249 140 61
BseYI C'CCAG_C
Cuts at: 0 488 564 1089
Size: 488 76 525
Fragments arranged by size:
525 488 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 1089
Size: 527 562
BsiHKAI G_wGCw'C
Cuts at: 0 302 392 1089
Size: 302 90 697
Fragments arranged by size:
697 302 90
Bsp1286I G_dGCh'C
Cuts at: 0 156 245 302 392 1089
Size: 156 89 57 90 697
Fragments arranged by size:
697 156 90 89 57
BspHI T'CATG_A
Cuts at: 0 302 1089
Size: 302 787
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 19 193 663 1089
Size: 19 174 470 426
Fragments arranged by size:
470 426 174 19
BsrFI r'CCGG_y
Cuts at: 0 1049 1089
Size: 1049 40
BssSI C'ACGA_G
Cuts at: 0 437 1089
Size: 437 652
BstEII G'GTnAC_C
Cuts at: 0 1002 1089
Size: 1002 87
BstYI r'GATC_y
Cuts at: 0 779 950 1089
Size: 779 171 139
Fragments arranged by size:
779 171 139
Bsu36I CC'TnA_GG
Cuts at: 0 44 1089
Size: 44 1045
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 129 164 1089
Size: 129 35 925
Fragments arranged by size:
925 129 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 697 1089
Size: 697 392
EaeI y'GGCC_r
Cuts at: 0 170 402 597 644 993 1089
Size: 170 232 195 47 349 96
Fragments arranged by size:
349 232 195 170 96 47
EarI CTCTTCn'nnn_
Cuts at: 0 374 540 1089
Size: 374 166 549
Fragments arranged by size:
549 374 166
EcoICRI GAG'CTC
Cuts at: 0 300 1089
Size: 300 789
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 655 760 859 1006 1089
Size: 483 172 105 99 147 83
Fragments arranged by size:
483 172 147 105 99 83
EcoO109I rG'GnC_Cy
Cuts at: 0 51 242 713 1089
Size: 51 191 471 376
Fragments arranged by size:
471 376 191 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 318 350 477 509 658 690 771 803
Size: 318 32 127 32 149 32 81 32
Cuts at: 803 1089
Size: 286
Fragments arranged by size:
318 286 149 127 81 32 32 32
32
HincII GTy'rAC
Cuts at: 0 801 1089
Size: 801 288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 1089
Size: 113 976
MscI TGG'CCA
Cuts at: 0 404 599 646 995 1089
Size: 404 195 47 349 94
Fragments arranged by size:
404 349 195 94 47
MslI CAynn'nnrTG
Cuts at: 0 307 1089
Size: 307 782
MspA1I CmG'CkG
Cuts at: 0 771 1089
Size: 771 318
NaeI GCC'GGC
Cuts at: 0 1051 1089
Size: 1051 38
NgoMIV G'CCGG_C
Cuts at: 0 1049 1089
Size: 1049 40
NspI r_CATG'y
Cuts at: 0 1078 1089
Size: 1078 11
PasI CC'CwG_GG
Cuts at: 0 63 94 1089
Size: 63 31 995
Fragments arranged by size:
995 63 31
PflMI CCAn_nnn'nTGG
Cuts at: 0 882 1089
Size: 882 207
PpuMI rG'GwC_Cy
Cuts at: 0 51 1089
Size: 51 1038
PspOMI G'GGCC_C
Cuts at: 0 241 1089
Size: 241 848
PvuII CAG'CTG
Cuts at: 0 771 1089
Size: 771 318
SacI G_AGCT'C
Cuts at: 0 302 1089
Size: 302 787
SapI GCTCTTCn'nnn_
Cuts at: 0 374 1089
Size: 374 715
SexAI A'CCwGG_T
Cuts at: 0 881 1089
Size: 881 208
SfcI C'TryA_G
Cuts at: 0 278 1089
Size: 278 811
SmlI C'TyrA_G
Cuts at: 0 324 1089
Size: 324 765
StuI AGG'CCT
Cuts at: 0 82 478 755 1089
Size: 82 396 277 334
Fragments arranged by size:
396 334 277 82
StyI C'CwwG_G
Cuts at: 0 399 1089
Size: 399 690
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 537 981 1089
Size: 537 444 108
Fragments arranged by size:
537 444 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 512 543 544 575 928 960 1089
Size: 512 31 1 31 353 32 129
Fragments arranged by size:
512 353 129 32 31 31 1
Tth111I GACn'n_nGTC
Cuts at: 0 672 1089
Size: 672 417
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 235 1089
Size: 235 854
Enzymes that do cut:
AccI AlfI AlwNI ApaI ApoI BanI BanII BbsI
BbvCI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspHI BspMI BsrFI BssSI BstEII BstYI Bsu36I CspCI
DrdI EaeI EarI EcoICRI Eco57MI EcoO109I Hin4I HincII
MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI
PflMI PpuMI PspOMI PvuII SacI SapI SexAI SfcI
SmlI StuI StyI TaqII TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmtI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrGI
BssHII BstAPI BstBI BstXI BstZ17I BtgI BtgZI BtsI
ClaI DraI DraIII EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI
XbaI XhoI XmaI XmnI ZraI