Restriction map for
pF1KB4033
(Linear) (Six-base) MAPSORT of: KIBB4033.seq Check: 4972 from: 1 to: 3786
KIBB4033.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 0 1943 2743 3786
Size: 1943 800 1043
Fragments arranged by size:
1943 1043 800
AccI GT'mk_AC
Cuts at: 0 397 2644 3786
Size: 397 2247 1142
Fragments arranged by size:
2247 1142 397
AclI AA'CG_TT
Cuts at: 0 1997 3786
Size: 1997 1789
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 596 910 1046 1583 2734 3071 3361
Size: 59 537 314 136 537 1151 337 290
Cuts at: 3361 3786
Size: 425
Fragments arranged by size:
1151 537 537 425 337 314 290 136
59
AflII C'TTAA_G
Cuts at: 0 1945 3786
Size: 1945 1841
AgeI A'CCGG_T
Cuts at: 0 2078 3786
Size: 2078 1708
AleI CACnn'nnGTG
Cuts at: 0 221 3552 3786
Size: 221 3331 234
Fragments arranged by size:
3331 234 221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3598 3786
Size: 3598 188
AlwNI CAG_nnn'CTG
Cuts at: 0 1385 3534 3786
Size: 1385 2149 252
Fragments arranged by size:
2149 1385 252
ApoI r'AATT_y
Cuts at: 0 27 70 118 2029 3786
Size: 27 43 48 1911 1757
Fragments arranged by size:
1911 1757 48 43 27
AvaI C'yCGr_G
Cuts at: 0 2960 3786
Size: 2960 826
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1110 1143 3786
Size: 1110 33 2643
Fragments arranged by size:
2643 1110 33
BamHI G'GATC_C
Cuts at: 0 1543 2084 3786
Size: 1543 541 1702
Fragments arranged by size:
1702 1543 541
BanI G'GyrC_C
Cuts at: 0 841 1390 2157 3786
Size: 841 549 767 1629
Fragments arranged by size:
1629 841 767 549
BbsI GAAGACnn'nnnn_
Cuts at: 0 477 2791 2834 3384 3619 3722 3736 3751
Size: 477 2314 43 550 235 103 14 15
Cuts at: 3751 3786
Size: 35
Fragments arranged by size:
2314 550 477 235 103 43 35 15
14
BbvCI CC'TCA_GC
Cuts at: 0 989 3786
Size: 989 2797
BciVI GTATCCnnnnn_n'
Cuts at: 0 464 2543 3786
Size: 464 2079 1243
Fragments arranged by size:
2079 1243 464
BclI T'GATC_A
Cuts at: 0 2501 2853 3485 3786
Size: 2501 352 632 301
Fragments arranged by size:
2501 632 352 301
BfrBI ATG'CAT
Cuts at: 0 1439 3786
Size: 1439 2347
BglI GCCn_nnn'nGGC
Cuts at: 0 2728 3786
Size: 2728 1058
BglII A'GATC_T
Cuts at: 0 1481 1488 3786
Size: 1481 7 2298
Fragments arranged by size:
2298 1481 7
Bme1580I G_kGCm'C
Cuts at: 0 1238 1393 3577 3786
Size: 1238 155 2184 209
Fragments arranged by size:
2184 1238 209 155
BmrI ACTGGGnnnn_n'
Cuts at: 0 307 1311 1395 2798 3786
Size: 307 1004 84 1403 988
Fragments arranged by size:
1403 1004 988 307 84
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3310 3786
Size: 3310 476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1221 1993 2791 3786
Size: 1221 772 798 995
Fragments arranged by size:
1221 995 798 772
Bpu10I CC'TnA_GC
Cuts at: 0 989 1607 2674 2900 3786
Size: 989 618 1067 226 886
Fragments arranged by size:
1067 989 886 618 226
BsaI GGTCTCn'nnnn_
Cuts at: 0 2501 3685 3786
Size: 2501 1184 101
Fragments arranged by size:
2501 1184 101
BsaAI yAC'GTr
Cuts at: 0 1357 3786
Size: 1357 2429
BsaHI Gr'CG_yC
Cuts at: 0 1940 2740 3786
Size: 1940 800 1046
Fragments arranged by size:
1940 1046 800
BsaWI w'CCGG_w
Cuts at: 0 2078 3786
Size: 2078 1708
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 660 690 3786
Size: 660 30 3096
Fragments arranged by size:
3096 660 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1205 1441 2779 3786
Size: 1205 236 1338 1007
Fragments arranged by size:
1338 1205 1007 236
BseYI C'CCAG_C
Cuts at: 0 1237 3786
Size: 1237 2549
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2480 3786
Size: 2480 1306
BsmI GAATG_Cn'
Cuts at: 0 94 2399 2666 3543 3786
Size: 94 2305 267 877 243
Fragments arranged by size:
2305 877 267 243 94
Bsp1286I G_dGCh'C
Cuts at: 0 1238 1393 3577 3786
Size: 1238 155 2184 209
Fragments arranged by size:
2184 1238 209 155
BsrDI GCAATG_nn'
Cuts at: 0 1667 3786
Size: 1667 2119
BsrFI r'CCGG_y
Cuts at: 0 2078 3786
Size: 2078 1708
BsrGI T'GTAC_A
Cuts at: 0 2843 3786
Size: 2843 943
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2999 3786
Size: 2999 787
BstBI TT'CG_AA
Cuts at: 0 2628 3786
Size: 2628 1158
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1852 2788 2802 3786
Size: 1852 936 14 984
Fragments arranged by size:
1852 984 936 14
BstYI r'GATC_y
Cuts at: 0 606 821 1481 1488 1543 1827 2084 2565
Size: 606 215 660 7 55 284 257 481
Cuts at: 2565 3184 3214 3786
Size: 619 30 572
Fragments arranged by size:
660 619 606 572 481 284 257 215
55 30 7
Bsu36I CC'TnA_GG
Cuts at: 0 1279 3786
Size: 1279 2507
BtgI C'CryG_G
Cuts at: 0 261 1724 1963 3786
Size: 261 1463 239 1823
Fragments arranged by size:
1823 1463 261 239
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1430 3786
Size: 1430 2356
BtsI GCAGTG_nn'
Cuts at: 0 1238 2738 2766 3279 3786
Size: 1238 1500 28 513 507
Fragments arranged by size:
1500 1238 513 507 28
DraI TTT'AAA
Cuts at: 0 1920 3335 3786
Size: 1920 1415 451
Fragments arranged by size:
1920 1415 451
DraIII CAC_nnn'GTG
Cuts at: 0 1534 3457 3786
Size: 1534 1923 329
Fragments arranged by size:
1923 1534 329
DrdI GACnn_nn'nnGTC
Cuts at: 0 1000 3786
Size: 1000 2786
EaeI y'GGCC_r
Cuts at: 0 323 414 1721 1791 3786
Size: 323 91 1307 70 1995
Fragments arranged by size:
1995 1307 323 91 70
EarI CTCTTCn'nnn_
Cuts at: 0 38 1919 2490 3337 3509 3786
Size: 38 1881 571 847 172 277
Fragments arranged by size:
1881 847 571 277 172 38
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1468 2824 3786
Size: 1468 1356 962
Fragments arranged by size:
1468 1356 962
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 596 910 1046 1221 1583 1993 2734
Size: 59 537 314 136 175 362 410 741
Cuts at: 2734 2791 3071 3361 3786
Size: 57 280 290 425
Fragments arranged by size:
741 537 425 410 362 314 290 280
175 136 59 57
EcoO109I rG'GnC_Cy
Cuts at: 0 869 1305 3786
Size: 869 436 2481
Fragments arranged by size:
2481 869 436
EcoRV GAT'ATC
Cuts at: 0 2586 3786
Size: 2586 1200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1174 1563 2739 3135 3786
Size: 1174 389 1176 396 651
Fragments arranged by size:
1176 1174 651 396 389
FspI TGC'GCA
Cuts at: 0 2876 3786
Size: 2876 910
HaeII r_GCGC'y
Cuts at: 0 911 3786
Size: 911 2875
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 660 692 749 781 1466 1473
Size: 379 32 249 32 57 32 685 7
Cuts at: 1473 1498 1505 2575 2607 3786
Size: 25 7 1070 32 1179
Fragments arranged by size:
1179 1070 685 379 249 57 32 32
32 32 25 7 7
HincII GTy'rAC
Cuts at: 0 237 927 1509 3786
Size: 237 690 582 2277
Fragments arranged by size:
2277 690 582 237
MfeI C'AATT_G
Cuts at: 0 365 2193 3786
Size: 365 1828 1593
Fragments arranged by size:
1828 1593 365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 3037 3305 3786
Size: 593 2444 268 481
Fragments arranged by size:
2444 593 481 268
MscI TGG'CCA
Cuts at: 0 325 1723 1793 3786
Size: 325 1398 70 1993
Fragments arranged by size:
1993 1398 325 70
MslI CAynn'nnrTG
Cuts at: 0 221 772 2800 2894 3552 3724 3786
Size: 221 551 2028 94 658 172 62
Fragments arranged by size:
2028 658 551 221 172 94 62
MspA1I CmG'CkG
Cuts at: 0 1241 3786
Size: 1241 2545
NcoI C'CATG_G
Cuts at: 0 261 1963 3786
Size: 261 1702 1823
Fragments arranged by size:
1823 1702 261
NdeI CA'TA_TG
Cuts at: 0 3243 3786
Size: 3243 543
NsiI A_TGCA'T
Cuts at: 0 1441 3786
Size: 1441 2345
NspI r_CATG'y
Cuts at: 0 554 1599 3786
Size: 554 1045 2187
Fragments arranged by size:
2187 1045 554
PflMI CCAn_nnn'nTGG
Cuts at: 0 680 1697 2940 3786
Size: 680 1017 1243 846
Fragments arranged by size:
1243 1017 846 680
PfoI T'CCnGG_A
Cuts at: 0 3296 3786
Size: 3296 490
PmlI CAC'GTG
Cuts at: 0 1357 3786
Size: 1357 2429
PpuMI rG'GwC_Cy
Cuts at: 0 1305 3786
Size: 1305 2481
PsiI TTA'TAA
Cuts at: 0 593 3786
Size: 593 3193
PstI C_TGCA'G
Cuts at: 0 1662 3468 3786
Size: 1662 1806 318
Fragments arranged by size:
1806 1662 318
PvuII CAG'CTG
Cuts at: 0 1241 3786
Size: 1241 2545
SanDI GG'GwC_CC
Cuts at: 0 1305 3786
Size: 1305 2481
ScaI AGT'ACT
Cuts at: 0 3501 3786
Size: 3501 285
SfcI C'TryA_G
Cuts at: 0 1658 2645 3464 3526 3786
Size: 1658 987 819 62 260
Fragments arranged by size:
1658 987 819 260 62
SmlI C'TyrA_G
Cuts at: 0 1945 3786
Size: 1945 1841
SphI G_CATG'C
Cuts at: 0 1599 3786
Size: 1599 2187
StuI AGG'CCT
Cuts at: 0 509 3786
Size: 509 3277
StyI C'CwwG_G
Cuts at: 0 261 1963 3621 3644 3786
Size: 261 1702 1658 23 142
Fragments arranged by size:
1702 1658 261 142 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2525 3631 3786
Size: 2525 1106 155
Fragments arranged by size:
2525 1106 155
TatI w'GTAC_w
Cuts at: 0 863 1953 2843 3032 3499 3786
Size: 863 1090 890 189 467 287
Fragments arranged by size:
1090 890 863 467 287 189
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1091 1123 3786
Size: 1091 32 2663
Fragments arranged by size:
2663 1091 32
XmnI GAAnn'nnTTC
Cuts at: 0 833 3786
Size: 833 2953
ZraI GAC'GTC
Cuts at: 0 1941 2741 3786
Size: 1941 800 1045
Fragments arranged by size:
1941 1045 800
Enzymes that do cut:
AatII AccI AclI AcuI AflII AgeI AleI AlfI
AlwNI ApoI AvaI BaeI BamHI BanI BbsI BbvCI
BciVI BclI BfrBI BglI BglII Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsmI Bsp1286I BsrDI BsrFI BsrGI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRV
FalI FspI HaeII Hin4I HincII MfeI MmeI MscI
MslI MspA1I NcoI NdeI NsiI NspI PflMI PfoI
PmlI PpuMI PsiI PstI PvuII SanDI ScaI SfcI
SmlI SphI StuI StyI TaqII TatI TstI XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AfeI AflIII AhdI AloI ApaI ApaLI
AscI AseI AsiSI AvrII BanII BbeI BcgI BlpI
BmgBI BmtI BpuEI BsaBI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BssHII BssSI BstEII BstZ17I
ClaI CspCI EagI EcoICRI EcoNI EcoRI FseI FspAI
HindIII HpaI KasI KpnI MluI NaeI NarI NgoMIV
NheI NotI NruI PacI PasI PciI PmeI PpiI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XcmI XhoI XmaI