(Linear) (Six-base) MAPSORT of: KIBB4028.seq  Check: 6462  from: 1  to: 1683



KIBB4028.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AclI AA'CG_TT

Cuts at:      0     735    1683 
   Size:        735     948
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1007    1392    1409    1683 
   Size:       1007     385      17     274

  Fragments arranged by size: 

                1007     385     274      17
 
AgeI A'CCGG_T

Cuts at:      0     143    1683 
   Size:        143    1540
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1235    1267    1683 
   Size:       1235      32     416

  Fragments arranged by size: 

                1235     416      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      54     969    1683 
   Size:         54     915     714

  Fragments arranged by size: 

                 915     714      54
 
ApoI r'AATT_y

Cuts at:      0     265    1310    1434    1510    1683 
   Size:        265    1045     124      76     173

  Fragments arranged by size: 

                1045     265     173     124      76
 
BamHI G'GATC_C

Cuts at:      0     707    1683 
   Size:        707     976
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     878    1683 
   Size:        878     805
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     166     200    1683 
   Size:        166      34    1483

  Fragments arranged by size: 

                1483     166      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     109     372     851    1683 
   Size:        109     263     479     832

  Fragments arranged by size: 

                 832     479     263     109
 
BfrBI ATG'CAT

Cuts at:      0      91    1683 
   Size:         91    1592
 
BlpI GC'TnA_GC

Cuts at:      0    1597    1683 
   Size:       1597      86
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     771     832    1109    1683 
   Size:        771      61     277     574

  Fragments arranged by size: 

                 771     574     277      61
 
BsaBI GATnn'nnATC

Cuts at:      0    1364    1683 
   Size:       1364     319
 
BsaWI w'CCGG_w

Cuts at:      0     143    1683 
   Size:        143    1540
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1174    1177    1683 
   Size:       1174       3     506

  Fragments arranged by size: 

                1174     506       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619    1683 
   Size:        619    1064
 
BsmI GAATG_Cn'

Cuts at:      0      91     179     959    1683 
   Size:         91      88     780     724

  Fragments arranged by size: 

                 780     724      91      88
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1342    1683 
   Size:       1342     341
 
BsrDI GCAATG_nn'

Cuts at:      0     449    1493    1683 
   Size:        449    1044     190

  Fragments arranged by size: 

                1044     449     190
 
BsrFI r'CCGG_y

Cuts at:      0     143    1683 
   Size:        143    1540
 
BstYI r'GATC_y

Cuts at:      0     707    1683 
   Size:        707     976
 
Bsu36I CC'TnA_GG

Cuts at:      0     703    1683 
   Size:        703     980
 
BtgI C'CryG_G

Cuts at:      0     593    1233    1683 
   Size:        593     640     450

  Fragments arranged by size: 

                 640     593     450
 
BtsI GCAGTG_nn'

Cuts at:      0      33    1683 
   Size:         33    1650
 
ClaI AT'CG_AT

Cuts at:      0    1269    1683 
   Size:       1269     414
 
DraI TTT'AAA

Cuts at:      0     438     524     569    1683 
   Size:        438      86      45    1114

  Fragments arranged by size: 

                1114     438      86      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     771     832    1007    1109    1392    1409    1683 
   Size:        771      61     175     102     283      17     274

  Fragments arranged by size: 

                 771     283     274     175     102      61      17
 
EcoRI G'AATT_C

Cuts at:      0    1310    1434    1683 
   Size:       1310     124     249

  Fragments arranged by size: 

                1310     249     124
 
HaeII r_GCGC'y

Cuts at:      0      19    1683 
   Size:         19    1664
 
HincII GTy'rAC

Cuts at:      0     159    1683 
   Size:        159    1524
 
MfeI C'AATT_G

Cuts at:      0     860    1683 
   Size:        860     823
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     329    1683 
   Size:        329    1354
 
MslI CAynn'nnrTG

Cuts at:      0     311    1683 
   Size:        311    1372
 
MspA1I CmG'CkG

Cuts at:      0     165     819    1672    1683 
   Size:        165     654     853      11

  Fragments arranged by size: 

                 853     654     165      11
 
NcoI C'CATG_G

Cuts at:      0    1233    1683 
   Size:       1233     450
 
NdeI CA'TA_TG

Cuts at:      0     417    1683 
   Size:        417    1266
 
NsiI A_TGCA'T

Cuts at:      0      93    1683 
   Size:         93    1590
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1321    1683 
   Size:       1321     362
 
PfoI T'CCnGG_A

Cuts at:      0     543    1683 
   Size:        543    1140
 
PvuII CAG'CTG

Cuts at:      0     819    1672    1683 
   Size:        819     853      11

  Fragments arranged by size: 

                 853     819      11
 
StyI C'CwwG_G

Cuts at:      0     989    1233    1683 
   Size:        989     244     450

  Fragments arranged by size: 

                 989     450     244
 
TatI w'GTAC_w

Cuts at:      0     834    1683 
   Size:        834     849
 
XbaI T'CTAG_A

Cuts at:      0    1260    1683 
   Size:       1260     423
 
XmnI GAAnn'nnTTC

Cuts at:      0     397     959    1422    1683 
   Size:        397     562     463     261

  Fragments arranged by size: 

                 562     463     397     261
 

 Enzymes that do cut:

     AclI     AcuI     AgeI     AloI    AlwNI     ApoI    BamHI     BbsI
     BcgI    BciVI    BfrBI     BlpI     BpmI    BsaBI    BsaWI    BseRI
     BsgI     BsmI    BsmBI    BsrDI    BsrFI    BstYI   Bsu36I     BtgI
     BtsI     ClaI     DraI  Eco57MI    EcoRI    HaeII   HincII     MfeI
     MmeI     MslI   MspA1I     NcoI     NdeI     NsiI    PflMI     PfoI
    PvuII     StyI     TatI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AhdI
     AleI     AlfI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BanI    BanII     BbeI    BbvCI     BclI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI    CspCI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I  HindIII     HpaI
     KasI     KpnI     MluI     MscI     NaeI     NarI   NgoMIV     NheI
     NotI     NruI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI