Restriction map for
pF1KB4014
(Linear) (Six-base) MAPSORT of: KIBB4014.seq Check: 3231 from: 1 to: 1407
KIBB4014.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 753 1407
Size: 753 654
Acc65I G'GTAC_C
Cuts at: 0 927 1407
Size: 927 480
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 1144 1407
Size: 1007 137 263
Fragments arranged by size:
1007 263 137
AleI CACnn'nnGTG
Cuts at: 0 1238 1407
Size: 1238 169
AlwNI CAG_nnn'CTG
Cuts at: 0 172 340 394 706 754 1407
Size: 172 168 54 312 48 653
Fragments arranged by size:
653 312 172 168 54 48
ApoI r'AATT_y
Cuts at: 0 1249 1407
Size: 1249 158
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 152 185 1407
Size: 152 33 1222
Fragments arranged by size:
1222 152 33
BanI G'GyrC_C
Cuts at: 0 297 927 979 1313 1407
Size: 297 630 52 334 94
Fragments arranged by size:
630 334 297 94 52
BanII G_rGCy'C
Cuts at: 0 388 973 1407
Size: 388 585 434
Fragments arranged by size:
585 434 388
BbeI G_GCGC'C
Cuts at: 0 1317 1407
Size: 1317 90
BglI GCCn_nnn'nGGC
Cuts at: 0 977 1407
Size: 977 430
Bme1580I G_kGCm'C
Cuts at: 0 895 1261 1407
Size: 895 366 146
Fragments arranged by size:
895 366 146
BmrI ACTGGGnnnn_n'
Cuts at: 0 382 1407
Size: 382 1025
Bpu10I CC'TnA_GC
Cuts at: 0 416 1407
Size: 416 991
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 1407
Size: 263 1144
BsaI GGTCTCn'nnnn_
Cuts at: 0 698 741 1407
Size: 698 43 666
Fragments arranged by size:
698 666 43
BsaHI Gr'CG_yC
Cuts at: 0 1314 1407
Size: 1314 93
BsaWI w'CCGG_w
Cuts at: 0 960 1407
Size: 960 447
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1380 1407
Size: 1380 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 836 1031 1299 1407
Size: 836 195 268 108
Fragments arranged by size:
836 268 195 108
BseYI C'CCAG_C
Cuts at: 0 228 452 838 850 932 1407
Size: 228 224 386 12 82 475
Fragments arranged by size:
475 386 228 224 82 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 964 1407
Size: 629 335 443
Fragments arranged by size:
629 443 335
BsmBI CGTCTCn'nnnn_
Cuts at: 0 670 1407
Size: 670 737
Bsp1286I G_dGCh'C
Cuts at: 0 388 895 973 1261 1407
Size: 388 507 78 288 146
Fragments arranged by size:
507 388 288 146 78
BspHI T'CATG_A
Cuts at: 0 1280 1407
Size: 1280 127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 481 680 753 1407
Size: 481 199 73 654
Fragments arranged by size:
654 481 199 73
BsrDI GCAATG_nn'
Cuts at: 0 353 995 1373 1407
Size: 353 642 378 34
Fragments arranged by size:
642 378 353 34
BsrFI r'CCGG_y
Cuts at: 0 777 1271 1407
Size: 777 494 136
Fragments arranged by size:
777 494 136
BsrGI T'GTAC_A
Cuts at: 0 315 1407
Size: 315 1092
BssSI C'ACGA_G
Cuts at: 0 1134 1407
Size: 1134 273
BstAPI GCAn_nnn'nTGC
Cuts at: 0 643 754 1407
Size: 643 111 653
Fragments arranged by size:
653 643 111
BstYI r'GATC_y
Cuts at: 0 592 1407
Size: 592 815
DraI TTT'AAA
Cuts at: 0 1110 1407
Size: 1110 297
EarI CTCTTCn'nnn_
Cuts at: 0 182 1407
Size: 182 1225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 1144 1407
Size: 1007 137 263
Fragments arranged by size:
1007 263 137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1122 1407
Size: 1122 285
HaeII r_GCGC'y
Cuts at: 0 307 914 1317 1407
Size: 307 607 403 90
Fragments arranged by size:
607 403 307 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1053 1085 1198 1230 1407
Size: 1053 32 113 32 177
Fragments arranged by size:
1053 177 113 32 32
KasI G'GCGC_C
Cuts at: 0 1313 1407
Size: 1313 94
KpnI G_GTAC'C
Cuts at: 0 931 1407
Size: 931 476
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 1407
Size: 100 1307
MslI CAynn'nnrTG
Cuts at: 0 539 922 1238 1407
Size: 539 383 316 169
Fragments arranged by size:
539 383 316 169
MspA1I CmG'CkG
Cuts at: 0 445 577 1166 1407
Size: 445 132 589 241
Fragments arranged by size:
589 445 241 132
NarI GG'CG_CC
Cuts at: 0 1314 1407
Size: 1314 93
NspI r_CATG'y
Cuts at: 0 1309 1407
Size: 1309 98
PflMI CCAn_nnn'nTGG
Cuts at: 0 1093 1407
Size: 1093 314
PfoI T'CCnGG_A
Cuts at: 0 52 201 1407
Size: 52 149 1206
Fragments arranged by size:
1206 149 52
PshAI GACnn'nnGTC
Cuts at: 0 829 1407
Size: 829 578
PstI C_TGCA'G
Cuts at: 0 69 118 691 750 759 1407
Size: 69 49 573 59 9 648
Fragments arranged by size:
648 573 69 59 49 9
PvuII CAG'CTG
Cuts at: 0 445 1166 1407
Size: 445 721 241
Fragments arranged by size:
721 445 241
SfcI C'TryA_G
Cuts at: 0 65 89 114 687 746 755 1077 1167
Size: 65 24 25 573 59 9 322 90
Cuts at: 1167 1407
Size: 240
Fragments arranged by size:
573 322 240 90 65 59 25 24
9
SfoI GGC'GCC
Cuts at: 0 1315 1407
Size: 1315 92
SmlI C'TyrA_G
Cuts at: 0 278 1407
Size: 278 1129
SphI G_CATG'C
Cuts at: 0 1309 1407
Size: 1309 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1100 1407
Size: 1100 307
TatI w'GTAC_w
Cuts at: 0 315 1407
Size: 315 1092
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1167 1199 1407
Size: 1167 32 208
Fragments arranged by size:
1167 208 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1090 1407
Size: 1090 317
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI ApoI BaeI BanI
BanII BbeI BglI Bme1580I BmrI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstYI
DraI EarI Eco57MI FalI HaeII Hin4I KasI KpnI
MmeI MslI MspA1I NarI NspI PflMI PfoI PshAI
PstI PvuII SfcI SfoI SmlI SphI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BpmI BsaAI BsaBI
BsiEI BsiHKAI BsiWI BsmI BspEI BsrBI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoO109I EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI StuI StyI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI