Restriction map for
pF1KB3985
(Linear) (Six-base) MAPSORT of: KIBB3985.seq Check: 2868 from: 1 to: 1008
KIBB3985.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 0 554 1008
Size: 554 454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 779 1008
Size: 616 163 229
Fragments arranged by size:
616 229 163
AfeI AGC'GCT
Cuts at: 0 41 1008
Size: 41 967
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 544 1008
Size: 544 464
AlwNI CAG_nnn'CTG
Cuts at: 0 284 585 1008
Size: 284 301 423
Fragments arranged by size:
423 301 284
ApaI G_GGCC'C
Cuts at: 0 517 1008
Size: 517 491
ApaLI G'TGCA_C
Cuts at: 0 54 1008
Size: 54 954
AvaI C'yCGr_G
Cuts at: 0 202 1008
Size: 202 806
BamHI G'GATC_C
Cuts at: 0 452 780 1008
Size: 452 328 228
Fragments arranged by size:
452 328 228
BanI G'GyrC_C
Cuts at: 0 493 759 1008
Size: 493 266 249
Fragments arranged by size:
493 266 249
BanII G_rGCy'C
Cuts at: 0 517 644 731 1008
Size: 517 127 87 277
Fragments arranged by size:
517 277 127 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 1008
Size: 10 998
BciVI GTATCCnnnnn_n'
Cuts at: 0 229 1008
Size: 229 779
BglI GCCn_nnn'nGGC
Cuts at: 0 89 1008
Size: 89 919
BlpI GC'TnA_GC
Cuts at: 0 691 1008
Size: 691 317
Bme1580I G_kGCm'C
Cuts at: 0 58 291 496 517 764 924 1008
Size: 58 233 205 21 247 160 84
Fragments arranged by size:
247 233 205 160 84 58 21
BmgBI CAC'GTC
Cuts at: 0 402 1008
Size: 402 606
BmrI ACTGGGnnnn_n'
Cuts at: 0 927 1008
Size: 927 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 745 1008
Size: 745 263
Bpu10I CC'TnA_GC
Cuts at: 0 303 574 1008
Size: 303 271 434
Fragments arranged by size:
434 303 271
BsaBI GATnn'nnATC
Cuts at: 0 785 1008
Size: 785 223
BsaHI Gr'CG_yC
Cuts at: 0 148 374 1008
Size: 148 226 634
Fragments arranged by size:
634 226 148
BseYI C'CCAG_C
Cuts at: 0 191 517 1008
Size: 191 326 491
Fragments arranged by size:
491 326 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 1008
Size: 301 707
BsiHKAI G_wGCw'C
Cuts at: 0 58 644 731 1008
Size: 58 586 87 277
Fragments arranged by size:
586 277 87 58
BsmI GAATG_Cn'
Cuts at: 0 435 1008
Size: 435 573
Bsp1286I G_dGCh'C
Cuts at: 0 58 291 496 517 644 731 764 924
Size: 58 233 205 21 127 87 33 160
Cuts at: 924 1008
Size: 84
Fragments arranged by size:
233 205 160 127 87 84 58 33
21
BspHI T'CATG_A
Cuts at: 0 605 1008
Size: 605 403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 482 572 1008
Size: 482 90 436
Fragments arranged by size:
482 436 90
BsrBI CCG'CTC
Cuts at: 0 102 1008
Size: 102 906
BsrFI r'CCGG_y
Cuts at: 0 157 171 1008
Size: 157 14 837
Fragments arranged by size:
837 157 14
BsrGI T'GTAC_A
Cuts at: 0 74 838 1008
Size: 74 764 170
Fragments arranged by size:
764 170 74
BstAPI GCAn_nnn'nTGC
Cuts at: 0 315 1008
Size: 315 693
BstEII G'GTnAC_C
Cuts at: 0 294 1008
Size: 294 714
BstYI r'GATC_y
Cuts at: 0 452 780 1008
Size: 452 328 228
Fragments arranged by size:
452 328 228
Bsu36I CC'TnA_GG
Cuts at: 0 421 1008
Size: 421 587
BtgI C'CryG_G
Cuts at: 0 18 212 220 1008
Size: 18 194 8 788
Fragments arranged by size:
788 194 18 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 414 1008
Size: 414 594
BtsI GCAGTG_nn'
Cuts at: 0 35 273 1008
Size: 35 238 735
Fragments arranged by size:
735 238 35
DraIII CAC_nnn'GTG
Cuts at: 0 53 1008
Size: 53 955
EaeI y'GGCC_r
Cuts at: 0 90 152 223 1008
Size: 90 62 71 785
Fragments arranged by size:
785 90 71 62
EarI CTCTTCn'nnn_
Cuts at: 0 649 1008
Size: 649 359
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 193 265 1008
Size: 193 72 743
Fragments arranged by size:
743 193 72
EcoICRI GAG'CTC
Cuts at: 0 642 729 1008
Size: 642 87 279
Fragments arranged by size:
642 279 87
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 745 779 1008
Size: 616 129 34 229
Fragments arranged by size:
616 229 129 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 577 1008
Size: 577 431
EcoO109I rG'GnC_Cy
Cuts at: 0 207 513 514 1008
Size: 207 306 1 494
Fragments arranged by size:
494 306 207 1
EcoRV GAT'ATC
Cuts at: 0 677 1008
Size: 677 331
HaeII r_GCGC'y
Cuts at: 0 43 201 1008
Size: 43 158 807
Fragments arranged by size:
807 158 43
MfeI C'AATT_G
Cuts at: 0 716 1008
Size: 716 292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 1008
Size: 704 304
MslI CAynn'nnrTG
Cuts at: 0 49 364 812 1008
Size: 49 315 448 196
Fragments arranged by size:
448 315 196 49
MspA1I CmG'CkG
Cuts at: 0 214 222 1008
Size: 214 8 786
Fragments arranged by size:
786 214 8
NaeI GCC'GGC
Cuts at: 0 173 1008
Size: 173 835
NcoI C'CATG_G
Cuts at: 0 18 1008
Size: 18 990
NgoMIV G'CCGG_C
Cuts at: 0 171 1008
Size: 171 837
NspI r_CATG'y
Cuts at: 0 531 1008
Size: 531 477
PflMI CCAn_nnn'nTGG
Cuts at: 0 24 686 1008
Size: 24 662 322
Fragments arranged by size:
662 322 24
PpuMI rG'GwC_Cy
Cuts at: 0 207 1008
Size: 207 801
PspOMI G'GGCC_C
Cuts at: 0 513 1008
Size: 513 495
PstI C_TGCA'G
Cuts at: 0 280 632 778 1008
Size: 280 352 146 230
Fragments arranged by size:
352 280 230 146
SacI G_AGCT'C
Cuts at: 0 644 731 1008
Size: 644 87 277
Fragments arranged by size:
644 277 87
SacII CC_GC'GG
Cuts at: 0 215 223 1008
Size: 215 8 785
Fragments arranged by size:
785 215 8
SanDI GG'GwC_CC
Cuts at: 0 207 1008
Size: 207 801
SapI GCTCTTCn'nnn_
Cuts at: 0 649 1008
Size: 649 359
SfcI C'TryA_G
Cuts at: 0 276 628 774 1008
Size: 276 352 146 234
Fragments arranged by size:
352 276 234 146
SmaI CCC'GGG
Cuts at: 0 204 1008
Size: 204 804
SspI AAT'ATT
Cuts at: 0 936 1008
Size: 936 72
StuI AGG'CCT
Cuts at: 0 601 1008
Size: 601 407
StyI C'CwwG_G
Cuts at: 0 18 1008
Size: 18 990
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 205 1008
Size: 205 803
TatI w'GTAC_w
Cuts at: 0 74 838 1008
Size: 74 764 170
Fragments arranged by size:
764 170 74
XmaI C'CCGG_G
Cuts at: 0 202 1008
Size: 202 806
XmnI GAAnn'nnTTC
Cuts at: 0 601 1008
Size: 601 407
Enzymes that do cut:
AccI AcuI AfeI AlfI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbsI BciVI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaBI BsaHI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI
BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
HaeII MfeI MmeI MslI MspA1I NaeI NcoI NgoMIV
NspI PflMI PpuMI PspOMI PstI SacI SacII SanDI
SapI SfcI SmaI SspI StuI StyI TaqII TatI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BclI BfrBI BglII BmtI BplI
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BsiEI BsiWI
BsmBI BspEI BsrDI BssHII BssSI BstBI BstXI BstZ17I
ClaI CspCI DraI DrdI EagI EcoRI FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MluI MscI NarI NdeI NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SwaI TstI Tth111I XbaI XcmI
XhoI ZraI