(Linear) (Six-base) MAPSORT of: KIBB3969.seq  Check: 2514  from: 1  to: 576



KIBB3969.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AccI GT'mk_AC

Cuts at:      0     125     192     576 
   Size:        125      67     384

  Fragments arranged by size: 

                 384     125      67
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     319     416     576 
   Size:        119     200      97     160

  Fragments arranged by size: 

                 200     160     119      97
 
AleI CACnn'nnGTG

Cuts at:      0     408     576 
   Size:        408     168
 
BsaBI GATnn'nnATC

Cuts at:      0      36     576 
   Size:         36     540
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     167     576 
   Size:        167     409
 
BsrGI T'GTAC_A

Cuts at:      0     254     576 
   Size:        254     322
 
BstZ17I GTA'TAC

Cuts at:      0     126     576 
   Size:        126     450
 
BtsI GCAGTG_nn'

Cuts at:      0     415     576 
   Size:        415     161
 
ClaI AT'CG_AT

Cuts at:      0     311     576 
   Size:        311     265
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119     319     416     576 
   Size:        119     200      97     160

  Fragments arranged by size: 

                 200     160     119      97
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     548     576 
   Size:        548      28
 
HindIII A'AGCT_T

Cuts at:      0     525     576 
   Size:        525      51
 
MfeI C'AATT_G

Cuts at:      0     341     576 
   Size:        341     235
 
MslI CAynn'nnrTG

Cuts at:      0     176     408     576 
   Size:        176     232     168

  Fragments arranged by size: 

                 232     176     168
 
SfcI C'TryA_G

Cuts at:      0     193     576 
   Size:        193     383
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     164     576 
   Size:        164     412
 
TatI w'GTAC_w

Cuts at:      0     254     576 
   Size:        254     322
 
XmnI GAAnn'nnTTC

Cuts at:      0     278     576 
   Size:        278     298
 

 Enzymes that do cut:

     AccI     AcuI     AleI    BsaBI    BseRI    BsrGI  BstZ17I     BtsI
     ClaI  Eco57MI     FalI  HindIII     MfeI     MslI     SfcI    TaqII
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI