Restriction map for
pF1KB3968
(Linear) (Six-base) MAPSORT of: KIBB3968.seq Check: 1359 from: 1 to: 1335
KIBB3968.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 0 347 1184 1335
Size: 347 837 151
Fragments arranged by size:
837 347 151
AflIII A'CryG_T
Cuts at: 0 584 1335
Size: 584 751
AgeI A'CCGG_T
Cuts at: 0 497 1335
Size: 497 838
AhdI GACnn_n'nnGTC
Cuts at: 0 57 1335
Size: 57 1278
AlwNI CAG_nnn'CTG
Cuts at: 0 139 1161 1239 1335
Size: 139 1022 78 96
Fragments arranged by size:
1022 139 96 78
ApaI G_GGCC'C
Cuts at: 0 174 1335
Size: 174 1161
AvaI C'yCGr_G
Cuts at: 0 46 1335
Size: 46 1289
BanII G_rGCy'C
Cuts at: 0 174 917 1335
Size: 174 743 418
Fragments arranged by size:
743 418 174
BbsI GAAGACnn'nnnn_
Cuts at: 0 179 778 1335
Size: 179 599 557
Fragments arranged by size:
599 557 179
BbvCI CC'TCA_GC
Cuts at: 0 10 1335
Size: 10 1325
BglI GCCn_nnn'nGGC
Cuts at: 0 1130 1335
Size: 1130 205
BlpI GC'TnA_GC
Cuts at: 0 382 1335
Size: 382 953
Bme1580I G_kGCm'C
Cuts at: 0 174 1335
Size: 174 1161
Bpu10I CC'TnA_GC
Cuts at: 0 10 1335
Size: 10 1325
BsaI GGTCTCn'nnnn_
Cuts at: 0 1292 1335
Size: 1292 43
BsaBI GATnn'nnATC
Cuts at: 0 286 1335
Size: 286 1049
BsaWI w'CCGG_w
Cuts at: 0 497 989 1335
Size: 497 492 346
Fragments arranged by size:
497 492 346
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 49 79 1335
Size: 49 30 1256
Fragments arranged by size:
1256 49 30
Bsp1286I G_dGCh'C
Cuts at: 0 174 917 1335
Size: 174 743 418
Fragments arranged by size:
743 418 174
BspEI T'CCGG_A
Cuts at: 0 989 1335
Size: 989 346
BsrDI GCAATG_nn'
Cuts at: 0 607 1335
Size: 607 728
BsrFI r'CCGG_y
Cuts at: 0 33 497 1335
Size: 33 464 838
Fragments arranged by size:
838 464 33
BstZ17I GTA'TAC
Cuts at: 0 1185 1335
Size: 1185 150
BtgI C'CryG_G
Cuts at: 0 1125 1335
Size: 1125 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1002 1173 1335
Size: 1002 171 162
Fragments arranged by size:
1002 171 162
DraI TTT'AAA
Cuts at: 0 1092 1335
Size: 1092 243
EaeI y'GGCC_r
Cuts at: 0 230 1122 1166 1249 1335
Size: 230 892 44 83 86
Fragments arranged by size:
892 230 86 83 44
EarI CTCTTCn'nnn_
Cuts at: 0 461 1335
Size: 461 874
EcoNI CCTnn'n_nnAGG
Cuts at: 0 372 1335
Size: 372 963
EcoO109I rG'GnC_Cy
Cuts at: 0 159 170 171 1207 1335
Size: 159 11 1 1036 128
Fragments arranged by size:
1036 159 128 11 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 49 75 79 81 111 845 877
Size: 43 6 26 4 2 30 734 32
Cuts at: 877 1335
Size: 458
Fragments arranged by size:
734 458 43 32 30 26 6 4
2
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 721 1335
Size: 203 518 614
Fragments arranged by size:
614 518 203
MscI TGG'CCA
Cuts at: 0 232 1124 1168 1335
Size: 232 892 44 167
Fragments arranged by size:
892 232 167 44
MslI CAynn'nnrTG
Cuts at: 0 356 1335
Size: 356 979
MspA1I CmG'CkG
Cuts at: 0 386 1335
Size: 386 949
NcoI C'CATG_G
Cuts at: 0 1125 1335
Size: 1125 210
NdeI CA'TA_TG
Cuts at: 0 357 1335
Size: 357 978
NspI r_CATG'y
Cuts at: 0 540 588 1335
Size: 540 48 747
Fragments arranged by size:
747 540 48
PciI A'CATG_T
Cuts at: 0 584 1335
Size: 584 751
PpuMI rG'GwC_Cy
Cuts at: 0 159 1335
Size: 159 1176
PspOMI G'GGCC_C
Cuts at: 0 170 1335
Size: 170 1165
PstI C_TGCA'G
Cuts at: 0 144 578 938 1335
Size: 144 434 360 397
Fragments arranged by size:
434 397 360 144
PvuII CAG'CTG
Cuts at: 0 386 1335
Size: 386 949
RsrII CG'GwC_CG
Cuts at: 0 987 1335
Size: 987 348
SapI GCTCTTCn'nnn_
Cuts at: 0 461 1335
Size: 461 874
SbfI CC_TGCA'GG
Cuts at: 0 938 1335
Size: 938 397
SfcI C'TryA_G
Cuts at: 0 140 574 934 1335
Size: 140 434 360 401
Fragments arranged by size:
434 401 360 140
StyI C'CwwG_G
Cuts at: 0 1125 1335
Size: 1125 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 198 1335
Size: 198 1137
TatI w'GTAC_w
Cuts at: 0 543 875 941 1335
Size: 543 332 66 394
Fragments arranged by size:
543 394 332 66
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 970 1335
Size: 970 365
Enzymes that do cut:
AccI AflIII AgeI AhdI AlwNI ApaI AvaI BanII
BbsI BbvCI BglI BlpI Bme1580I Bpu10I BsaI BsaBI
BsaWI BsaXI Bsp1286I BspEI BsrDI BsrFI BstZ17I BtgI
BtgZI DraI EaeI EarI EcoNI EcoO109I Hin4I MmeI
MscI MslI MspA1I NcoI NdeI NspI PciI PpuMI
PspOMI PstI PvuII RsrII SapI SbfI SfcI StyI
TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI
BglII BmgBI BmrI BmtI BplI BpmI BpuEI BsaAI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtsI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI Eco57MI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI SacI
SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI