(Linear) (Six-base) MAPSORT of: KIBB3938.seq  Check: 8356  from: 1  to: 1326



KIBB3938.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     210    1326 
   Size:        210    1116
 
AccI GT'mk_AC

Cuts at:      0     476    1326 
   Size:        476     850
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     725    1054    1326 
   Size:        725     329     272

  Fragments arranged by size: 

                 725     329     272
 
AleI CACnn'nnGTG

Cuts at:      0     316    1326 
   Size:        316    1010
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     623     655    1326 
   Size:        623      32     671

  Fragments arranged by size: 

                 671     623      32
 
ApoI r'AATT_y

Cuts at:      0    1131    1326 
   Size:       1131     195
 
BanI G'GyrC_C

Cuts at:      0     198     210     528    1211    1326 
   Size:        198      12     318     683     115

  Fragments arranged by size: 

                 683     318     198     115      12
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     790    1326 
   Size:        790     536
 
BbvCI CC'TCA_GC

Cuts at:      0    1021    1326 
   Size:       1021     305
 
BclI T'GATC_A

Cuts at:      0     936    1278    1326 
   Size:        936     342      48

  Fragments arranged by size: 

                 936     342      48
 
BglI GCCn_nnn'nGGC

Cuts at:      0     184     427    1326 
   Size:        184     243     899

  Fragments arranged by size: 

                 899     243     184
 
Bpu10I CC'TnA_GC

Cuts at:      0    1021    1326 
   Size:       1021     305
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     431     687    1326 
   Size:        431     256     639

  Fragments arranged by size: 

                 639     431     256
 
BsaHI Gr'CG_yC

Cuts at:      0     139    1326 
   Size:        139    1187
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      94    1326 
   Size:         94    1232
 
BseYI C'CCAG_C

Cuts at:      0     339    1326 
   Size:        339     987
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430    1326 
   Size:        430     896
 
BsiEI CG_ry'CG

Cuts at:      0     193    1326 
   Size:        193    1133
 
BsiHKAI G_wGCw'C

Cuts at:      0     582    1326 
   Size:        582     744
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     945    1326 
   Size:        945     381
 
Bsp1286I G_dGCh'C

Cuts at:      0     582    1326 
   Size:        582     744
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     210     449    1326 
   Size:        210     239     877

  Fragments arranged by size: 

                 877     239     210
 
BstYI r'GATC_y

Cuts at:      0      68     235    1326 
   Size:         68     167    1091

  Fragments arranged by size: 

                1091     167      68
 
BtgI C'CryG_G

Cuts at:      0     551    1326 
   Size:        551     775
 
BtsI GCAGTG_nn'

Cuts at:      0     863    1326 
   Size:        863     463
 
DraI TTT'AAA

Cuts at:      0     932    1326 
   Size:        932     394
 
EaeI y'GGCC_r

Cuts at:      0     499     857     962    1326 
   Size:        499     358     105     364

  Fragments arranged by size: 

                 499     364     358     105
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     230    1326 
   Size:        230    1096
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     725    1054    1326 
   Size:        725     329     272

  Fragments arranged by size: 

                 725     329     272
 
EcoO109I rG'GnC_Cy

Cuts at:      0     274    1326 
   Size:        274    1052
 
EcoRI G'AATT_C

Cuts at:      0    1131    1326 
   Size:       1131     195
 
EcoRV GAT'ATC

Cuts at:      0     598    1326 
   Size:        598     728
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1215    1326 
   Size:       1215     111
 
MfeI C'AATT_G

Cuts at:      0    1148    1326 
   Size:       1148     178
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     197    1326 
   Size:        197    1129
 
MscI TGG'CCA

Cuts at:      0     501     859     964    1326 
   Size:        501     358     105     362

  Fragments arranged by size: 

                 501     362     358     105
 
MslI CAynn'nnrTG

Cuts at:      0     316    1200    1286    1326 
   Size:        316     884      86      40

  Fragments arranged by size: 

                 884     316      86      40
 
NspI r_CATG'y

Cuts at:      0     396     921    1326 
   Size:        396     525     405

  Fragments arranged by size: 

                 525     405     396
 
PpuMI rG'GwC_Cy

Cuts at:      0     274    1326 
   Size:        274    1052
 
PsiI TTA'TAA

Cuts at:      0    1049    1326 
   Size:       1049     277
 
SphI G_CATG'C

Cuts at:      0     396     921    1326 
   Size:        396     525     405

  Fragments arranged by size: 

                 525     405     396
 
StyI C'CwwG_G

Cuts at:      0     164     270    1238    1326 
   Size:        164     106     968      88

  Fragments arranged by size: 

                 968     164     106      88
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     159     666    1326 
   Size:        159     507     660

  Fragments arranged by size: 

                 660     507     159
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     548     580    1326 
   Size:        548      32     746

  Fragments arranged by size: 

                 746     548      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     180     429    1326 
   Size:        180     249     897

  Fragments arranged by size: 

                 897     249     180
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI     AloI     ApoI     BanI     BbsI
    BbvCI     BclI     BglI   Bpu10I     BsaI    BsaHI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BstYI     BtgI
     BtsI     DraI     EaeI     EciI  Eco57MI EcoO109I    EcoRI    EcoRV
     FalI     MfeI     MmeI     MscI     MslI     NspI    PpuMI     PsiI
     SphI     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI    BanII     BbeI     BcgI    BciVI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI    BsiWI     BsmI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
   DraIII     DrdI     EagI     EarI  EcoICRI    EcoNI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI