Restriction map for
pF1KB3919
(Linear) (Six-base) MAPSORT of: KIBB3919.seq Check: 8746 from: 1 to: 1737
KIBB3919.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 0 870 1737
Size: 870 867
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 770 1121 1154 1522 1737
Size: 232 538 351 33 368 215
Fragments arranged by size:
538 368 351 232 215 33
AflIII A'CryG_T
Cuts at: 0 116 1003 1737
Size: 116 887 734
Fragments arranged by size:
887 734 116
ApaI G_GGCC'C
Cuts at: 0 150 1166 1737
Size: 150 1016 571
Fragments arranged by size:
1016 571 150
ApoI r'AATT_y
Cuts at: 0 435 814 1406 1542 1655 1664 1737
Size: 435 379 592 136 113 9 73
Fragments arranged by size:
592 435 379 136 113 73 9
AvaI C'yCGr_G
Cuts at: 0 503 1737
Size: 503 1234
BamHI G'GATC_C
Cuts at: 0 556 1737
Size: 556 1181
BanI G'GyrC_C
Cuts at: 0 643 754 1252 1737
Size: 643 111 498 485
Fragments arranged by size:
643 498 485 111
BanII G_rGCy'C
Cuts at: 0 150 535 1166 1737
Size: 150 385 631 571
Fragments arranged by size:
631 571 385 150
BbsI GAAGACnn'nnnn_
Cuts at: 0 112 1737
Size: 112 1625
BbvCI CC'TCA_GC
Cuts at: 0 25 1016 1737
Size: 25 991 721
Fragments arranged by size:
991 721 25
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 220 254 1276 1310 1737
Size: 220 34 1022 34 427
Fragments arranged by size:
1022 427 220 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 573 1737
Size: 573 1164
BfrBI ATG'CAT
Cuts at: 0 792 1737
Size: 792 945
BglI GCCn_nnn'nGGC
Cuts at: 0 154 163 1737
Size: 154 9 1574
Fragments arranged by size:
1574 154 9
Bme1580I G_kGCm'C
Cuts at: 0 150 1166 1737
Size: 150 1016 571
Fragments arranged by size:
1016 571 150
BmrI ACTGGGnnnn_n'
Cuts at: 0 669 1737
Size: 669 1068
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 802 1310 1325 1737
Size: 536 266 508 15 412
Fragments arranged by size:
536 508 412 266 15
Bpu10I CC'TnA_GC
Cuts at: 0 25 1016 1397 1737
Size: 25 991 381 340
Fragments arranged by size:
991 381 340 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 522 788 1737
Size: 136 386 266 949
Fragments arranged by size:
949 386 266 136
BsaI GGTCTCn'nnnn_
Cuts at: 0 554 1737
Size: 554 1183
BsaBI GATnn'nnATC
Cuts at: 0 561 733 1737
Size: 561 172 1004
Fragments arranged by size:
1004 561 172
BsaHI Gr'CG_yC
Cuts at: 0 71 867 1737
Size: 71 796 870
Fragments arranged by size:
870 796 71
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 921 951 1737
Size: 921 30 786
Fragments arranged by size:
921 786 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 525 1033 1036 1172 1737
Size: 525 508 3 136 565
Fragments arranged by size:
565 525 508 136 3
BseYI C'CCAG_C
Cuts at: 0 143 479 1236 1737
Size: 143 336 757 501
Fragments arranged by size:
757 501 336 143
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 1574 1737
Size: 172 1402 163
Fragments arranged by size:
1402 172 163
BsiHKAI G_wGCw'C
Cuts at: 0 215 1737
Size: 215 1522
Bsp1286I G_dGCh'C
Cuts at: 0 150 215 535 1166 1737
Size: 150 65 320 631 571
Fragments arranged by size:
631 571 320 150 65
BspHI T'CATG_A
Cuts at: 0 1028 1737
Size: 1028 709
BsrDI GCAATG_nn'
Cuts at: 0 940 999 1737
Size: 940 59 738
Fragments arranged by size:
940 738 59
BssSI C'ACGA_G
Cuts at: 0 1571 1737
Size: 1571 166
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1342 1737
Size: 1342 395
BstXI CCAn_nnnn'nTGG
Cuts at: 0 619 1737
Size: 619 1118
BstYI r'GATC_y
Cuts at: 0 77 556 827 1737
Size: 77 479 271 910
Fragments arranged by size:
910 479 271 77
Bsu36I CC'TnA_GG
Cuts at: 0 1158 1737
Size: 1158 579
ClaI AT'CG_AT
Cuts at: 0 686 1737
Size: 686 1051
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 597 632 1737
Size: 597 35 1105
Fragments arranged by size:
1105 597 35
EaeI y'GGCC_r
Cuts at: 0 473 1737
Size: 473 1264
EarI CTCTTCn'nnn_
Cuts at: 0 818 1445 1737
Size: 818 627 292
Fragments arranged by size:
818 627 292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 536 770 802 1121 1154 1310 1325
Size: 232 304 234 32 319 33 156 15
Cuts at: 1325 1522 1737
Size: 197 215
Fragments arranged by size:
319 304 234 232 215 197 156 33
32 15
EcoO109I rG'GnC_Cy
Cuts at: 0 91 147 1162 1163 1737
Size: 91 56 1015 1 574
Fragments arranged by size:
1015 574 91 56 1
EcoRV GAT'ATC
Cuts at: 0 935 1737
Size: 935 802
HaeII r_GCGC'y
Cuts at: 0 169 1737
Size: 169 1568
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 831 863 1134 1166 1209 1241 1737
Size: 831 32 271 32 43 32 496
Fragments arranged by size:
831 496 271 43 32 32 32
MluI A'CGCG_T
Cuts at: 0 116 1737
Size: 116 1621
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 600 843 1737
Size: 600 243 894
Fragments arranged by size:
894 600 243
MslI CAynn'nnrTG
Cuts at: 0 1628 1737
Size: 1628 109
MspA1I CmG'CkG
Cuts at: 0 1330 1737
Size: 1330 407
NsiI A_TGCA'T
Cuts at: 0 794 1737
Size: 794 943
NspI r_CATG'y
Cuts at: 0 1007 1737
Size: 1007 730
PciI A'CATG_T
Cuts at: 0 1003 1737
Size: 1003 734
PfoI T'CCnGG_A
Cuts at: 0 552 1095 1737
Size: 552 543 642
Fragments arranged by size:
642 552 543
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 331 363 1372 1404 1737
Size: 331 32 1009 32 333
Fragments arranged by size:
1009 333 331 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 91 1737
Size: 91 1646
PspOMI G'GGCC_C
Cuts at: 0 146 1162 1737
Size: 146 1016 575
Fragments arranged by size:
1016 575 146
PstI C_TGCA'G
Cuts at: 0 360 1737
Size: 360 1377
SanDI GG'GwC_CC
Cuts at: 0 91 1737
Size: 91 1646
SfcI C'TryA_G
Cuts at: 0 356 1737
Size: 356 1381
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 154 1737
Size: 154 1583
SmlI C'TyrA_G
Cuts at: 0 151 537 803 1737
Size: 151 386 266 934
Fragments arranged by size:
934 386 266 151
SpeI A'CTAG_T
Cuts at: 0 300 1737
Size: 300 1437
StyI C'CwwG_G
Cuts at: 0 1115 1737
Size: 1115 622
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 207 625 1146 1737
Size: 207 418 521 591
Fragments arranged by size:
591 521 418 207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 921 953 1737
Size: 921 32 784
Fragments arranged by size:
921 784 32
Tth111I GACn'n_nGTC
Cuts at: 0 675 1737
Size: 675 1062
ZraI GAC'GTC
Cuts at: 0 868 1737
Size: 868 869
Enzymes that do cut:
AatII AcuI AflIII ApaI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BfrBI BglI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BsrDI BssSI
BstAPI BstXI BstYI Bsu36I ClaI CspCI EaeI EarI
Eco57MI EcoO109I EcoRV HaeII Hin4I MluI MmeI MslI
MspA1I NsiI NspI PciI PfoI PpiI PpuMI PspOMI
PstI SanDI SfcI SfiI SmlI SpeI StyI TaqII
TstI Tth111I ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BclI BglII BlpI BmgBI BmtI
BplI BsaAI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstZ17I
BtgI BtgZI BtsI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI StuI SwaI TatI XbaI XcmI XhoI XmaI
XmnI