(Linear) (Six-base) MAPSORT of: KIBB3911.seq  Check: 8140  from: 1  to: 1362



KIBB3911.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     461    1362 
   Size:        461     901
 
ApaLI G'TGCA_C

Cuts at:      0      63    1362 
   Size:         63    1299
 
ApoI r'AATT_y

Cuts at:      0     200     610     713    1039    1362 
   Size:        200     410     103     326     323

  Fragments arranged by size: 

                 410     326     323     200     103
 
AvaI C'yCGr_G

Cuts at:      0     704    1004    1362 
   Size:        704     300     358

  Fragments arranged by size: 

                 704     358     300
 
BamHI G'GATC_C

Cuts at:      0     225    1362 
   Size:        225    1137
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1216    1362 
   Size:       1216     146
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     536    1216    1362 
   Size:        536     680     146

  Fragments arranged by size: 

                 680     536     146
 
BclI T'GATC_A

Cuts at:      0     795    1362 
   Size:        795     567
 
Bme1580I G_kGCm'C

Cuts at:      0      67    1362 
   Size:         67    1295
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     733    1362 
   Size:        733     629
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     741    1362 
   Size:        741     621
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     407    1362 
   Size:        407     955
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34    1362 
   Size:         34    1328
 
BseYI C'CCAG_C

Cuts at:      0    1304    1362 
   Size:       1304      58
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     488    1250    1362 
   Size:        488     762     112

  Fragments arranged by size: 

                 762     488     112
 
BsiHKAI G_wGCw'C

Cuts at:      0      67    1362 
   Size:         67    1295
 
Bsp1286I G_dGCh'C

Cuts at:      0      67    1362 
   Size:         67    1295
 
BspHI T'CATG_A

Cuts at:      0     195    1362 
   Size:        195    1167
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     354    1362 
   Size:        354    1008
 
BsrGI T'GTAC_A

Cuts at:      0     273     285    1187    1362 
   Size:        273      12     902     175

  Fragments arranged by size: 

                 902     273     175      12
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     975    1362 
   Size:        975     387
 
BstYI r'GATC_y

Cuts at:      0     225    1362 
   Size:        225    1137
 
BtgI C'CryG_G

Cuts at:      0    1293    1362 
   Size:       1293      69
 
DraI TTT'AAA

Cuts at:      0     152    1362 
   Size:        152    1210
 
EaeI y'GGCC_r

Cuts at:      0     951    1362 
   Size:        951     411
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     461     741    1362 
   Size:        461     280     621

  Fragments arranged by size: 

                 621     461     280
 
EcoRI G'AATT_C

Cuts at:      0     610    1362 
   Size:        610     752
 
EcoRV GAT'ATC

Cuts at:      0     216    1362 
   Size:        216    1146
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     424     553    1362 
   Size:        424     129     809

  Fragments arranged by size: 

                 809     424     129
 
HaeII r_GCGC'y

Cuts at:      0     898    1362 
   Size:        898     464
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     907     939    1362 
   Size:        907      32     423

  Fragments arranged by size: 

                 907     423      32
 
MfeI C'AATT_G

Cuts at:      0     791    1362 
   Size:        791     571
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     984    1362 
   Size:        984     378
 
MscI TGG'CCA

Cuts at:      0     953    1362 
   Size:        953     409
 
MslI CAynn'nnrTG

Cuts at:      0    1074    1362 
   Size:       1074     288
 
MspA1I CmG'CkG

Cuts at:      0     103     222     696    1234    1362 
   Size:        103     119     474     538     128

  Fragments arranged by size: 

                 538     474     128     119     103
 
NcoI C'CATG_G

Cuts at:      0    1293    1362 
   Size:       1293      69
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     563    1362 
   Size:        563     799
 
PsiI TTA'TAA

Cuts at:      0     859    1362 
   Size:        859     503
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     158     190    1362 
   Size:        158      32    1172

  Fragments arranged by size: 

                1172     158      32
 
PvuII CAG'CTG

Cuts at:      0     103     222    1362 
   Size:        103     119    1140

  Fragments arranged by size: 

                1140     119     103
 
SfcI C'TryA_G

Cuts at:      0     175     516    1362 
   Size:        175     341     846

  Fragments arranged by size: 

                 846     341     175
 
SmlI C'TyrA_G

Cuts at:      0     422    1004    1362 
   Size:        422     582     358

  Fragments arranged by size: 

                 582     422     358
 
StyI C'CwwG_G

Cuts at:      0     249     303     537    1293    1362 
   Size:        249      54     234     756      69

  Fragments arranged by size: 

                 756     249     234      69      54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     579     637    1362 
   Size:        579      58     725

  Fragments arranged by size: 

                 725     579      58
 
TatI w'GTAC_w

Cuts at:      0     273     285     885    1187    1362 
   Size:        273      12     600     302     175

  Fragments arranged by size: 

                 600     302     273     175      12
 
Tth111I GACn'n_nGTC

Cuts at:      0     577    1362 
   Size:        577     785
 
XhoI C'TCGA_G

Cuts at:      0    1004    1362 
   Size:       1004     358
 
XmnI GAAnn'nnTTC

Cuts at:      0     610    1362 
   Size:        610     752
 

 Enzymes that do cut:

     AcuI    ApaLI     ApoI     AvaI    BamHI     BbsI    BciVI     BclI
 Bme1580I     BplI     BpmI    BpuEI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspHI    BspMI    BsrGI   BstAPI    BstYI     BtgI     DraI
     EaeI  Eco57MI    EcoRI    EcoRV     FalI    HaeII    Hin4I     MfeI
     MmeI     MscI     MslI   MspA1I     NcoI    PflMI     PsiI     PsrI
    PvuII     SfcI     SmlI     StyI    TaqII     TatI  Tth111I     XhoI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BsrBI    BsrDI    BsrFI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI     XbaI
     XcmI     XmaI     ZraI