Restriction map for
pF1KB3902
(Linear) (Six-base) MAPSORT of: KIBB3902.seq Check: 9097 from: 1 to: 1836
KIBB3902.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AatII G_ACGT'C
Cuts at: 0 1827 1836
Size: 1827 9
AccI GT'mk_AC
Cuts at: 0 902 1371 1836
Size: 902 469 465
Fragments arranged by size:
902 469 465
AflIII A'CryG_T
Cuts at: 0 915 1580 1836
Size: 915 665 256
Fragments arranged by size:
915 665 256
AhdI GACnn_n'nnGTC
Cuts at: 0 449 1836
Size: 449 1387
AleI CACnn'nnGTG
Cuts at: 0 426 1836
Size: 426 1410
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1667 1699 1836
Size: 1667 32 137
Fragments arranged by size:
1667 137 32
AlwNI CAG_nnn'CTG
Cuts at: 0 447 1023 1836
Size: 447 576 813
Fragments arranged by size:
813 576 447
ApaI G_GGCC'C
Cuts at: 0 1493 1836
Size: 1493 343
ApoI r'AATT_y
Cuts at: 0 1381 1836
Size: 1381 455
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 192 225 1836
Size: 192 33 1611
Fragments arranged by size:
1611 192 33
BanII G_rGCy'C
Cuts at: 0 82 302 1196 1493 1836
Size: 82 220 894 297 343
Fragments arranged by size:
894 343 297 220 82
BbsI GAAGACnn'nnnn_
Cuts at: 0 262 445 1286 1836
Size: 262 183 841 550
Fragments arranged by size:
841 550 262 183
BciVI GTATCCnnnnn_n'
Cuts at: 0 1305 1836
Size: 1305 531
BclI T'GATC_A
Cuts at: 0 1355 1836
Size: 1355 481
Bme1580I G_kGCm'C
Cuts at: 0 1156 1493 1836
Size: 1156 337 343
Fragments arranged by size:
1156 343 337
BmgBI CAC'GTC
Cuts at: 0 648 848 1836
Size: 648 200 988
Fragments arranged by size:
988 648 200
BmrI ACTGGGnnnn_n'
Cuts at: 0 746 1159 1536 1836
Size: 746 413 377 300
Fragments arranged by size:
746 413 377 300
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1443 1836
Size: 1443 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 970 1836
Size: 949 21 866
Fragments arranged by size:
949 866 21
Bpu10I CC'TnA_GC
Cuts at: 0 938 1836
Size: 938 898
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 213 1171 1836
Size: 213 958 665
Fragments arranged by size:
958 665 213
BsaAI yAC'GTr
Cuts at: 0 916 1583 1836
Size: 916 667 253
Fragments arranged by size:
916 667 253
BsaHI Gr'CG_yC
Cuts at: 0 1824 1836
Size: 1824 12
BsaWI w'CCGG_w
Cuts at: 0 307 1836
Size: 307 1529
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 946 1435 1836
Size: 946 489 401
Fragments arranged by size:
946 489 401
BseYI C'CCAG_C
Cuts at: 0 18 51 584 1836
Size: 18 33 533 1252
Fragments arranged by size:
1252 533 33 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 982 991 1426 1836
Size: 982 9 435 410
Fragments arranged by size:
982 435 410 9
BsiEI CG_ry'CG
Cuts at: 0 142 1836
Size: 142 1694
BsiHKAI G_wGCw'C
Cuts at: 0 302 956 1210 1836
Size: 302 654 254 626
Fragments arranged by size:
654 626 302 254
BsiWI C'GTAC_G
Cuts at: 0 660 1836
Size: 660 1176
BsmI GAATG_Cn'
Cuts at: 0 350 1836
Size: 350 1486
Bsp1286I G_dGCh'C
Cuts at: 0 82 302 956 1156 1196 1210 1493 1836
Size: 82 220 654 200 40 14 283 343
Fragments arranged by size:
654 343 283 220 200 82 40 14
BspHI T'CATG_A
Cuts at: 0 1352 1836
Size: 1352 484
BsrDI GCAATG_nn'
Cuts at: 0 201 699 851 885 1133 1836
Size: 201 498 152 34 248 703
Fragments arranged by size:
703 498 248 201 152 34
BsrFI r'CCGG_y
Cuts at: 0 134 410 1836
Size: 134 276 1426
Fragments arranged by size:
1426 276 134
BssSI C'ACGA_G
Cuts at: 0 426 1836
Size: 426 1410
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1005 1023 1836
Size: 1005 18 813
Fragments arranged by size:
1005 813 18
Bsu36I CC'TnA_GG
Cuts at: 0 1679 1836
Size: 1679 157
BtgI C'CryG_G
Cuts at: 0 287 1400 1455 1779 1836
Size: 287 1113 55 324 57
Fragments arranged by size:
1113 324 287 57 55
BtsI GCAGTG_nn'
Cuts at: 0 954 1438 1836
Size: 954 484 398
Fragments arranged by size:
954 484 398
DrdI GACnn_nn'nnGTC
Cuts at: 0 730 1501 1585 1836
Size: 730 771 84 251
Fragments arranged by size:
771 730 251 84
EaeI y'GGCC_r
Cuts at: 0 139 1176 1836
Size: 139 1037 660
Fragments arranged by size:
1037 660 139
EagI C'GGCC_G
Cuts at: 0 139 1836
Size: 139 1697
EarI CTCTTCn'nnn_
Cuts at: 0 163 442 601 1096 1351 1836
Size: 163 279 159 495 255 485
Fragments arranged by size:
495 485 279 255 163 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 54 1836
Size: 54 1782
EcoICRI GAG'CTC
Cuts at: 0 300 1836
Size: 300 1536
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 970 1836
Size: 949 21 866
Fragments arranged by size:
949 866 21
EcoO109I rG'GnC_Cy
Cuts at: 0 1396 1836
Size: 1396 440
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1212 1836
Size: 1212 624
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 435 467 1836
Size: 435 32 1369
Fragments arranged by size:
1369 435 32
HincII GTy'rAC
Cuts at: 0 651 1372 1836
Size: 651 721 464
Fragments arranged by size:
721 651 464
MfeI C'AATT_G
Cuts at: 0 129 1836
Size: 129 1707
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 1287 1836
Size: 449 838 549
Fragments arranged by size:
838 549 449
MscI TGG'CCA
Cuts at: 0 1178 1836
Size: 1178 658
MslI CAynn'nnrTG
Cuts at: 0 426 1045 1510 1836
Size: 426 619 465 326
Fragments arranged by size:
619 465 426 326
MspA1I CmG'CkG
Cuts at: 0 494 506 1836
Size: 494 12 1330
Fragments arranged by size:
1330 494 12
NaeI GCC'GGC
Cuts at: 0 136 1836
Size: 136 1700
NcoI C'CATG_G
Cuts at: 0 1779 1836
Size: 1779 57
NgoMIV G'CCGG_C
Cuts at: 0 134 1836
Size: 134 1702
PfoI T'CCnGG_A
Cuts at: 0 167 1755 1836
Size: 167 1588 81
Fragments arranged by size:
1588 167 81
PmlI CAC'GTG
Cuts at: 0 916 1583 1836
Size: 916 667 253
Fragments arranged by size:
916 667 253
PpuMI rG'GwC_Cy
Cuts at: 0 1396 1836
Size: 1396 440
PspOMI G'GGCC_C
Cuts at: 0 1489 1836
Size: 1489 347
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1528 1560 1836
Size: 1528 32 276
Fragments arranged by size:
1528 276 32
PstI C_TGCA'G
Cuts at: 0 115 493 746 1836
Size: 115 378 253 1090
Fragments arranged by size:
1090 378 253 115
PvuII CAG'CTG
Cuts at: 0 494 1836
Size: 494 1342
SacI G_AGCT'C
Cuts at: 0 302 1836
Size: 302 1534
SalI G'TCGA_C
Cuts at: 0 1370 1836
Size: 1370 466
SanDI GG'GwC_CC
Cuts at: 0 1396 1836
Size: 1396 440
SapI GCTCTTCn'nnn_
Cuts at: 0 442 1096 1836
Size: 442 654 740
Fragments arranged by size:
740 654 442
ScaI AGT'ACT
Cuts at: 0 1546 1836
Size: 1546 290
SfcI C'TryA_G
Cuts at: 0 111 489 742 1836
Size: 111 378 253 1094
Fragments arranged by size:
1094 378 253 111
SmlI C'TyrA_G
Cuts at: 0 228 1186 1836
Size: 228 958 650
Fragments arranged by size:
958 650 228
StyI C'CwwG_G
Cuts at: 0 1708 1779 1836
Size: 1708 71 57
Fragments arranged by size:
1708 71 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1120 1836
Size: 1120 716
TatI w'GTAC_w
Cuts at: 0 1544 1836
Size: 1544 292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 998 1114 1146 1171 1836
Size: 998 116 32 25 665
Fragments arranged by size:
998 665 116 32 25
XmnI GAAnn'nnTTC
Cuts at: 0 157 481 1836
Size: 157 324 1355
Fragments arranged by size:
1355 324 157
ZraI GAC'GTC
Cuts at: 0 1825 1836
Size: 1825 11
Enzymes that do cut:
AatII AccI AflIII AhdI AleI AloI AlwNI ApaI
ApoI BaeI BanII BbsI BciVI BclI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I
BspHI BsrDI BsrFI BssSI BstAPI Bsu36I BtgI BtsI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
FalI Hin4I HincII MfeI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV PfoI PmlI PpuMI PspOMI PsrI
PstI PvuII SacI SalI SanDI SapI ScaI SfcI
SmlI StyI TaqII TatI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AgeI AlfI
ApaLI AscI AseI AsiSI AvaI AvrII BamHI BanI
BbeI BbvCI BcgI BfrBI BglI BglII BlpI BmtI
BsaI BsaBI BsaXI BsmBI BspEI BspMI BsrBI BsrGI
BssHII BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI
CspCI DraI DraIII EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MluI NarI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspXI PvuI
RsrII SacII SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XbaI XhoI XmaI