Restriction map for
pF1KB3881
(Linear) (Six-base) MAPSORT of: KIBB3881.seq Check: 998 from: 1 to: 1107
KIBB3881.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AccI GT'mk_AC
Cuts at: 0 650 1107
Size: 650 457
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 625 733 1107
Size: 325 300 108 374
Fragments arranged by size:
374 325 300 108
AleI CACnn'nnGTG
Cuts at: 0 316 754 1107
Size: 316 438 353
Fragments arranged by size:
438 353 316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 28 889 1107
Size: 28 861 218
Fragments arranged by size:
861 218 28
ApaLI G'TGCA_C
Cuts at: 0 100 457 904 1107
Size: 100 357 447 203
Fragments arranged by size:
447 357 203 100
BamHI G'GATC_C
Cuts at: 0 614 641 1107
Size: 614 27 466
Fragments arranged by size:
614 466 27
BanII G_rGCy'C
Cuts at: 0 982 1107
Size: 982 125
BbvCI CC'TCA_GC
Cuts at: 0 233 1107
Size: 233 874
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 443 477 1107
Size: 443 34 630
Fragments arranged by size:
630 443 34
Bme1580I G_kGCm'C
Cuts at: 0 104 461 908 1107
Size: 104 357 447 199
Fragments arranged by size:
447 357 199 104
BmgBI CAC'GTC
Cuts at: 0 891 896 903 1107
Size: 891 5 7 204
Fragments arranged by size:
891 204 7 5
BmrI ACTGGGnnnn_n'
Cuts at: 0 581 1107
Size: 581 526
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 687 1107
Size: 687 420
Bpu10I CC'TnA_GC
Cuts at: 0 233 1107
Size: 233 874
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 868 1107
Size: 868 239
BsaAI yAC'GTr
Cuts at: 0 72 1107
Size: 72 1035
BsaHI Gr'CG_yC
Cuts at: 0 1013 1107
Size: 1013 94
BsaWI w'CCGG_w
Cuts at: 0 215 596 1107
Size: 215 381 511
Fragments arranged by size:
511 381 215
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 463 493 1107
Size: 463 30 614
Fragments arranged by size:
614 463 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 301 637 1107
Size: 250 51 336 470
Fragments arranged by size:
470 336 250 51
BsiHKAI G_wGCw'C
Cuts at: 0 7 104 461 908 1107
Size: 7 97 357 447 199
Fragments arranged by size:
447 357 199 97 7
Bsp1286I G_dGCh'C
Cuts at: 0 7 104 461 908 982 1107
Size: 7 97 357 447 74 125
Fragments arranged by size:
447 357 125 97 74 7
BspEI T'CCGG_A
Cuts at: 0 596 1107
Size: 596 511
BspHI T'CATG_A
Cuts at: 0 521 1107
Size: 521 586
BsrBI CCG'CTC
Cuts at: 0 422 612 1107
Size: 422 190 495
Fragments arranged by size:
495 422 190
BsrDI GCAATG_nn'
Cuts at: 0 529 828 1107
Size: 529 299 279
Fragments arranged by size:
529 299 279
BsrGI T'GTAC_A
Cuts at: 0 206 1107
Size: 206 901
BssHII G'CGCG_C
Cuts at: 0 722 923 1107
Size: 722 201 184
Fragments arranged by size:
722 201 184
BstAPI GCAn_nnn'nTGC
Cuts at: 0 498 1071 1095 1107
Size: 498 573 24 12
Fragments arranged by size:
573 498 24 12
BstYI r'GATC_y
Cuts at: 0 593 614 641 1107
Size: 593 21 27 466
Fragments arranged by size:
593 466 27 21
BstZ17I GTA'TAC
Cuts at: 0 651 1107
Size: 651 456
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 546 1107
Size: 546 561
BtsI GCAGTG_nn'
Cuts at: 0 85 459 927 1107
Size: 85 374 468 180
Fragments arranged by size:
468 374 180 85
DraIII CAC_nnn'GTG
Cuts at: 0 276 359 1107
Size: 276 83 748
Fragments arranged by size:
748 276 83
DrdI GACnn_nn'nnGTC
Cuts at: 0 580 1107
Size: 580 527
EaeI y'GGCC_r
Cuts at: 0 502 1107
Size: 502 605
EarI CTCTTCn'nnn_
Cuts at: 0 196 1107
Size: 196 911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 625 733 1107
Size: 325 300 108 374
Fragments arranged by size:
374 325 300 108
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 141 1107
Size: 141 966
FspI TGC'GCA
Cuts at: 0 850 1107
Size: 850 257
HincII GTy'rAC
Cuts at: 0 348 546 1107
Size: 348 198 561
Fragments arranged by size:
561 348 198
HindIII A'AGCT_T
Cuts at: 0 1027 1107
Size: 1027 80
MslI CAynn'nnrTG
Cuts at: 0 139 316 754 871 1107
Size: 139 177 438 117 236
Fragments arranged by size:
438 236 177 139 117
MspA1I CmG'CkG
Cuts at: 0 774 999 1107
Size: 774 225 108
Fragments arranged by size:
774 225 108
NdeI CA'TA_TG
Cuts at: 0 872 1107
Size: 872 235
NspI r_CATG'y
Cuts at: 0 858 1065 1107
Size: 858 207 42
Fragments arranged by size:
858 207 42
PasI CC'CwG_GG
Cuts at: 0 797 1107
Size: 797 310
PfoI T'CCnGG_A
Cuts at: 0 617 1107
Size: 617 490
PstI C_TGCA'G
Cuts at: 0 1079 1107
Size: 1079 28
ScaI AGT'ACT
Cuts at: 0 406 1107
Size: 406 701
SexAI A'CCwGG_T
Cuts at: 0 95 1107
Size: 95 1012
SfcI C'TryA_G
Cuts at: 0 1075 1107
Size: 1075 32
SmlI C'TyrA_G
Cuts at: 0 883 1107
Size: 883 224
SphI G_CATG'C
Cuts at: 0 1065 1107
Size: 1065 42
StuI AGG'CCT
Cuts at: 0 11 161 1107
Size: 11 150 946
Fragments arranged by size:
946 150 11
StyI C'CwwG_G
Cuts at: 0 764 1107
Size: 764 343
TatI w'GTAC_w
Cuts at: 0 206 404 1107
Size: 206 198 703
Fragments arranged by size:
703 206 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 880 912 1107
Size: 880 32 195
Fragments arranged by size:
880 195 32
Tth111I GACn'n_nGTC
Cuts at: 0 343 1107
Size: 343 764
Enzymes that do cut:
AccI AcuI AleI AlfI ApaLI BamHI BanII BbvCI
BcgI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BsiHKAI Bsp1286I BspEI BspHI
BsrBI BsrDI BsrGI BssHII BstAPI BstYI BstZ17I BtgZI
BtsI DraIII DrdI EaeI EarI Eco57MI FalI FspI
HincII HindIII MslI MspA1I NdeI NspI PasI PfoI
PstI ScaI SexAI SfcI SmlI SphI StuI StyI
TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbsI BciVI BclI
BfrBI BglI BglII BlpI BmtI BpmI BsaI BsaBI
BseYI BsgI BsiEI BsiWI BsmI BsmBI BspMI BsrFI
BssSI BstBI BstEII BstXI Bsu36I BtgI ClaI CspCI
DraI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspAI HaeII Hin4I HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TaqII XbaI XcmI XhoI XmaI XmnI
ZraI