Restriction map for
pF1KB3878
(Linear) (Six-base) MAPSORT of: KIBB3878.seq Check: 3758 from: 1 to: 1146
KIBB3878.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1061 1146
Size: 1061 85
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 555 1146
Size: 555 591
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 532 564 1146
Size: 532 32 582
Fragments arranged by size:
582 532 32
ApaI G_GGCC'C
Cuts at: 0 95 1146
Size: 95 1051
AvaI C'yCGr_G
Cuts at: 0 82 94 1146
Size: 82 12 1052
Fragments arranged by size:
1052 82 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 931 964 1146
Size: 931 33 182
Fragments arranged by size:
931 182 33
BanII G_rGCy'C
Cuts at: 0 95 350 1146
Size: 95 255 796
Fragments arranged by size:
796 255 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 561 624 1146
Size: 561 63 522
Fragments arranged by size:
561 522 63
BbvCI CC'TCA_GC
Cuts at: 0 140 526 1146
Size: 140 386 620
Fragments arranged by size:
620 386 140
BciVI GTATCCnnnnn_n'
Cuts at: 0 772 1146
Size: 772 374
BglI GCCn_nnn'nGGC
Cuts at: 0 49 1146
Size: 49 1097
BlpI GC'TnA_GC
Cuts at: 0 532 619 1146
Size: 532 87 527
Fragments arranged by size:
532 527 87
Bme1580I G_kGCm'C
Cuts at: 0 95 920 1146
Size: 95 825 226
Fragments arranged by size:
825 226 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 98 1146
Size: 98 1048
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 513 1146
Size: 513 633
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 310 457 534 538 589 601 1057
Size: 173 137 147 77 4 51 12 456
Cuts at: 1057 1146
Size: 89
Fragments arranged by size:
456 173 147 137 89 77 51 12
4
Bpu10I CC'TnA_GC
Cuts at: 0 140 526 1146
Size: 140 386 620
Fragments arranged by size:
620 386 140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 1146
Size: 643 503
BsaI GGTCTCn'nnnn_
Cuts at: 0 757 1146
Size: 757 389
BsaHI Gr'CG_yC
Cuts at: 0 629 1146
Size: 629 517
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 532 562 1146
Size: 532 30 584
Fragments arranged by size:
584 532 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 13 167 1017 1146
Size: 13 154 850 129
Fragments arranged by size:
850 154 129 13
BseYI C'CCAG_C
Cuts at: 0 130 1146
Size: 130 1016
BsiHKAI G_wGCw'C
Cuts at: 0 350 887 1146
Size: 350 537 259
Fragments arranged by size:
537 350 259
BsmI GAATG_Cn'
Cuts at: 0 232 375 1146
Size: 232 143 771
Fragments arranged by size:
771 232 143
Bsp1286I G_dGCh'C
Cuts at: 0 95 350 887 920 1146
Size: 95 255 537 33 226
Fragments arranged by size:
537 255 226 95 33
BspHI T'CATG_A
Cuts at: 0 1109 1146
Size: 1109 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 179 287 596 884 1061 1070 1146
Size: 179 108 309 288 177 9 76
Fragments arranged by size:
309 288 179 177 108 76 9
BstEII G'GTnAC_C
Cuts at: 0 5 1146
Size: 5 1141
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1101 1146
Size: 1101 45
BstYI r'GATC_y
Cuts at: 0 600 1146
Size: 600 546
BtgI C'CryG_G
Cuts at: 0 651 1136 1146
Size: 651 485 10
Fragments arranged by size:
651 485 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 399 1146
Size: 399 747
BtsI GCAGTG_nn'
Cuts at: 0 918 1146
Size: 918 228
EarI CTCTTCn'nnn_
Cuts at: 0 482 1146
Size: 482 664
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1111 1146
Size: 1111 35
EcoICRI GAG'CTC
Cuts at: 0 348 1146
Size: 348 798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 310 457 534 538 555 589 601
Size: 173 137 147 77 4 17 34 12
Cuts at: 601 1057 1146
Size: 456 89
Fragments arranged by size:
456 173 147 137 89 77 34 17
12 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 283 1146
Size: 283 863
EcoO109I rG'GnC_Cy
Cuts at: 0 193 288 492 717 1146
Size: 193 95 204 225 429
Fragments arranged by size:
429 225 204 193 95
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 876 1146
Size: 876 270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 1012 1044 1146
Size: 235 32 745 32 102
Fragments arranged by size:
745 235 102 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 896 1146
Size: 464 432 250
Fragments arranged by size:
464 432 250
MspA1I CmG'CkG
Cuts at: 0 525 1101 1146
Size: 525 576 45
Fragments arranged by size:
576 525 45
NcoI C'CATG_G
Cuts at: 0 651 1146
Size: 651 495
PasI CC'CwG_GG
Cuts at: 0 713 1146
Size: 713 433
PfoI T'CCnGG_A
Cuts at: 0 596 1146
Size: 596 550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 1146
Size: 532 32 582
Fragments arranged by size:
582 532 32
PpuMI rG'GwC_Cy
Cuts at: 0 288 492 717 1146
Size: 288 204 225 429
Fragments arranged by size:
429 288 225 204
PspOMI G'GGCC_C
Cuts at: 0 91 1146
Size: 91 1055
PstI C_TGCA'G
Cuts at: 0 1133 1146
Size: 1133 13
PvuII CAG'CTG
Cuts at: 0 525 1146
Size: 525 621
SacI G_AGCT'C
Cuts at: 0 350 1146
Size: 350 796
SanDI GG'GwC_CC
Cuts at: 0 717 1146
Size: 717 429
SfcI C'TryA_G
Cuts at: 0 1129 1146
Size: 1129 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 49 1146
Size: 49 1097
SmaI CCC'GGG
Cuts at: 0 84 96 1146
Size: 84 12 1050
Fragments arranged by size:
1050 84 12
SmlI C'TyrA_G
Cuts at: 0 658 1146
Size: 658 488
SrfI GCCC'GGGC
Cuts at: 0 96 1146
Size: 96 1050
StuI AGG'CCT
Cuts at: 0 380 1146
Size: 380 766
StyI C'CwwG_G
Cuts at: 0 54 651 1146
Size: 54 597 495
Fragments arranged by size:
597 495 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 144 1146
Size: 144 1002
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 897 929 1146
Size: 897 32 217
Fragments arranged by size:
897 217 32
Tth111I GACn'n_nGTC
Cuts at: 0 628 1146
Size: 628 518
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1005 1146
Size: 1005 141
XmaI C'CCGG_G
Cuts at: 0 82 94 1146
Size: 82 12 1052
Fragments arranged by size:
1052 82 12
XmnI GAAnn'nnTTC
Cuts at: 0 334 1146
Size: 334 812
Enzymes that do cut:
AarI AcuI AloI ApaI AvaI BaeI BanII BbsI
BbvCI BciVI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspHI BspMI BstEII BstXI BstYI BtgI
BtgZI BtsI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI Hin4I MmeI MspA1I NcoI PasI PfoI PpiI
PpuMI PspOMI PstI PvuII SacI SanDI SfcI SfiI
SmaI SmlI SrfI StuI StyI TaqII TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BamHI BanI BbeI BcgI BclI BfrBI
BglII BmgBI BmtI BsaAI BsaBI BsaWI BsgI BsiEI
BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EaeI EagI EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI TatI XbaI XhoI
ZraI