(Linear) (Six-base) MAPSORT of: KIBB3848.seq  Check: 5142  from: 1  to: 402



KIBB3848.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     402 
   Size:        235     167
 
AflIII A'CryG_T

Cuts at:      0     389     402 
   Size:        389      13
 
AvaI C'yCGr_G

Cuts at:      0     373     402 
   Size:        373      29
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     277     402 
   Size:        277     125
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      82      85     115     118     148     151     241     256 
   Size:         82       3      30       3      30       3      90      15

Cuts at:    256     337     340     343     352     402 
   Size:         81       3       3       9      50

  Fragments arranged by size: 

                  90      82      81      50      30      30      15       9
                   3       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0      57     402 
   Size:         57     345
 
BsiHKAI G_wGCw'C

Cuts at:      0     362     402 
   Size:        362      40
 
Bsp1286I G_dGCh'C

Cuts at:      0     362     402 
   Size:        362      40
 
BspHI T'CATG_A

Cuts at:      0      11     402 
   Size:         11     391
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     377     402 
   Size:        377      25
 
BsrBI CCG'CTC

Cuts at:      0     108     174     402 
   Size:        108      66     228

  Fragments arranged by size: 

                 228     108      66
 
BssHII G'CGCG_C

Cuts at:      0     157     402 
   Size:        157     245
 
BstYI r'GATC_y

Cuts at:      0     194     402 
   Size:        194     208
 
EarI CTCTTCn'nnn_

Cuts at:      0     329     402 
   Size:        329      73
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     184     402 
   Size:        184     218
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     235     277     402 
   Size:        235      42     125

  Fragments arranged by size: 

                 235     125      42
 
EcoO109I rG'GnC_Cy

Cuts at:      0      21      78     402 
   Size:         21      57     324

  Fragments arranged by size: 

                 324      57      21
 
HaeII r_GCGC'y

Cuts at:      0     121     402 
   Size:        121     281
 
MspA1I CmG'CkG

Cuts at:      0     381     402 
   Size:        381      21
 
NspI r_CATG'y

Cuts at:      0     393     402 
   Size:        393       9
 
PciI A'CATG_T

Cuts at:      0     389     402 
   Size:        389      13
 
PfoI T'CCnGG_A

Cuts at:      0     347     402 
   Size:        347      55
 
PpuMI rG'GwC_Cy

Cuts at:      0      78     402 
   Size:         78     324
 
PstI C_TGCA'G

Cuts at:      0     386     402 
   Size:        386      16
 
SfcI C'TryA_G

Cuts at:      0     382     402 
   Size:        382      20
 
SmaI CCC'GGG

Cuts at:      0     375     402 
   Size:        375      27
 
SrfI GCCC'GGGC

Cuts at:      0     375     402 
   Size:        375      27
 
StyI C'CwwG_G

Cuts at:      0      24     402 
   Size:         24     378
 
Tth111I GACn'n_nGTC

Cuts at:      0       7     402 
   Size:          7     395
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      42     402 
   Size:         42     360
 
XmaI C'CCGG_G

Cuts at:      0     373     402 
   Size:        373      29
 

 Enzymes that do cut:

     AcuI   AflIII     AvaI     BpmI    BseRI    BseYI  BsiHKAI Bsp1286I
    BspHI    BspMI    BsrBI   BssHII    BstYI     EarI     EciI  Eco57MI
 EcoO109I    HaeII   MspA1I     NspI     PciI     PfoI    PpuMI     PstI
     SfcI     SmaI     SrfI     StyI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     SwaI    TaqII     TatI
     TstI     XbaI     XhoI     XmnI     ZraI