Restriction map for
pF1KB3845
(Linear) (Six-base) MAPSORT of: KIBB3845.seq Check: 9101 from: 1 to: 1050
KIBB3845.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1050
Size: 481 569
AgeI A'CCGG_T
Cuts at: 0 172 1050
Size: 172 878
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 556 588 1050
Size: 556 32 462
Fragments arranged by size:
556 462 32
ApoI r'AATT_y
Cuts at: 0 655 700 718 1050
Size: 655 45 18 332
Fragments arranged by size:
655 332 45 18
BanI G'GyrC_C
Cuts at: 0 51 78 1050
Size: 51 27 972
Fragments arranged by size:
972 51 27
BanII G_rGCy'C
Cuts at: 0 14 62 422 1050
Size: 14 48 360 628
Fragments arranged by size:
628 360 48 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 166 175 1050
Size: 166 9 875
Fragments arranged by size:
875 166 9
BbvCI CC'TCA_GC
Cuts at: 0 586 1050
Size: 586 464
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 652 686 1050
Size: 652 34 364
Fragments arranged by size:
652 364 34
Bme1580I G_kGCm'C
Cuts at: 0 83 1050
Size: 83 967
BmrI ACTGGGnnnn_n'
Cuts at: 0 223 1050
Size: 223 827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 661 910 1050
Size: 255 406 249 140
Fragments arranged by size:
406 255 249 140
Bpu10I CC'TnA_GC
Cuts at: 0 56 68 586 620 1050
Size: 56 12 518 34 430
Fragments arranged by size:
518 430 56 34 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 919 1050
Size: 589 330 131
Fragments arranged by size:
589 330 131
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 383 1050
Size: 13 370 667
Fragments arranged by size:
667 370 13
BsaWI w'CCGG_w
Cuts at: 0 172 1008 1050
Size: 172 836 42
Fragments arranged by size:
836 172 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 634 664 1050
Size: 634 30 386
Fragments arranged by size:
634 386 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 301 481 658 1050
Size: 301 180 177 392
Fragments arranged by size:
392 301 180 177
BseYI C'CCAG_C
Cuts at: 0 114 413 1050
Size: 114 299 637
Fragments arranged by size:
637 299 114
BsiEI CG_ry'CG
Cuts at: 0 837 1050
Size: 837 213
Bsp1286I G_dGCh'C
Cuts at: 0 14 62 83 422 1050
Size: 14 48 21 339 628
Fragments arranged by size:
628 339 48 21 14
BspHI T'CATG_A
Cuts at: 0 575 1050
Size: 575 475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 119 1050
Size: 119 931
BsrDI GCAATG_nn'
Cuts at: 0 614 1050
Size: 614 436
BsrFI r'CCGG_y
Cuts at: 0 172 1050
Size: 172 878
BssSI C'ACGA_G
Cuts at: 0 506 1050
Size: 506 544
BstXI CCAn_nnnn'nTGG
Cuts at: 0 114 306 639 817 1050
Size: 114 192 333 178 233
Fragments arranged by size:
333 233 192 178 114
BstYI r'GATC_y
Cuts at: 0 293 564 1050
Size: 293 271 486
Fragments arranged by size:
486 293 271
BtgI C'CryG_G
Cuts at: 0 847 1050
Size: 847 203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 462 1050
Size: 462 588
BtsI GCAGTG_nn'
Cuts at: 0 513 1050
Size: 513 537
DrdI GACnn_nn'nnGTC
Cuts at: 0 508 1050
Size: 508 542
EaeI y'GGCC_r
Cuts at: 0 201 834 1050
Size: 201 633 216
Fragments arranged by size:
633 216 201
EagI C'GGCC_G
Cuts at: 0 834 1050
Size: 834 216
EarI CTCTTCn'nnn_
Cuts at: 0 247 1050
Size: 247 803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 481 661 910 1050
Size: 255 226 180 249 140
Fragments arranged by size:
255 249 226 180 140
EcoNI CCTnn'n_nnAGG
Cuts at: 0 463 589 1050
Size: 463 126 461
Fragments arranged by size:
463 461 126
EcoO109I rG'GnC_Cy
Cuts at: 0 828 917 930 1050
Size: 828 89 13 120
Fragments arranged by size:
828 120 89 13
EcoRI G'AATT_C
Cuts at: 0 655 700 1050
Size: 655 45 350
Fragments arranged by size:
655 350 45
FspI TGC'GCA
Cuts at: 0 154 1050
Size: 154 896
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 380 412 634 666 893 925 1050
Size: 380 32 222 32 227 32 125
Fragments arranged by size:
380 227 222 125 32 32 32
HincII GTy'rAC
Cuts at: 0 234 474 1050
Size: 234 240 576
Fragments arranged by size:
576 240 234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 1050
Size: 131 919
MscI TGG'CCA
Cuts at: 0 203 1050
Size: 203 847
MslI CAynn'nnrTG
Cuts at: 0 862 1050
Size: 862 188
MspA1I CmG'CkG
Cuts at: 0 76 275 1050
Size: 76 199 775
Fragments arranged by size:
775 199 76
NcoI C'CATG_G
Cuts at: 0 847 1050
Size: 847 203
NspI r_CATG'y
Cuts at: 0 331 777 1050
Size: 331 446 273
Fragments arranged by size:
446 331 273
PflMI CCAn_nnn'nTGG
Cuts at: 0 385 1050
Size: 385 665
PfoI T'CCnGG_A
Cuts at: 0 926 1050
Size: 926 124
PpuMI rG'GwC_Cy
Cuts at: 0 828 917 930 1050
Size: 828 89 13 120
Fragments arranged by size:
828 120 89 13
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 439 471 1050
Size: 439 32 579
Fragments arranged by size:
579 439 32
PvuII CAG'CTG
Cuts at: 0 275 1050
Size: 275 775
SapI GCTCTTCn'nnn_
Cuts at: 0 247 1050
Size: 247 803
SmlI C'TyrA_G
Cuts at: 0 568 934 1050
Size: 568 366 116
Fragments arranged by size:
568 366 116
SphI G_CATG'C
Cuts at: 0 777 1050
Size: 777 273
StuI AGG'CCT
Cuts at: 0 66 268 1001 1050
Size: 66 202 733 49
Fragments arranged by size:
733 202 66 49
StyI C'CwwG_G
Cuts at: 0 847 1050
Size: 847 203
TatI w'GTAC_w
Cuts at: 0 431 1050
Size: 431 619
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 938 970 1050
Size: 938 32 80
Fragments arranged by size:
938 80 32
Tth111I GACn'n_nGTC
Cuts at: 0 907 1050
Size: 907 143
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 45 1050
Size: 45 1005
Enzymes that do cut:
AcuI AgeI AloI ApoI BanI BanII BbsI BbvCI
BcgI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaWI
BsaXI BseRI BseYI BsiEI Bsp1286I BspHI BspMI BsrDI
BsrFI BssSI BstXI BstYI BtgI BtgZI BtsI DrdI
EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI FspI
Hin4I HincII MmeI MscI MslI MspA1I NcoI NspI
PflMI PfoI PpuMI PsrI PvuII SapI SmlI SphI
StuI StyI TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaAI
BsaBI BsaHI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI
BsrBI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII EciI EcoICRI EcoRV FalI
FseI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TaqII XbaI XhoI XmaI XmnI ZraI