Restriction map for
pF1KB3821
(Linear) (Six-base) MAPSORT of: KIBB3821.seq Check: 4163 from: 1 to: 1269
KIBB3821.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 80 1266 1269
Size: 80 1186 3
Fragments arranged by size:
1186 80 3
AseI AT'TA_AT
Cuts at: 0 214 1269
Size: 214 1055
BamHI G'GATC_C
Cuts at: 0 385 1269
Size: 385 884
BanI G'GyrC_C
Cuts at: 0 321 1269
Size: 321 948
BciVI GTATCCnnnnn_n'
Cuts at: 0 581 1269
Size: 581 688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 892 1269
Size: 102 790 377
Fragments arranged by size:
790 377 102
Bpu10I CC'TnA_GC
Cuts at: 0 1201 1269
Size: 1201 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 769 1043 1269
Size: 158 611 274 226
Fragments arranged by size:
611 274 226 158
BsaI GGTCTCn'nnnn_
Cuts at: 0 281 1269
Size: 281 988
BsaHI Gr'CG_yC
Cuts at: 0 47 1269
Size: 47 1222
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 75 105 571 601 677 707 1269
Size: 75 30 466 30 76 30 562
Fragments arranged by size:
562 466 76 75 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 685 1269
Size: 685 584
BseYI C'CCAG_C
Cuts at: 0 149 1269
Size: 149 1120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 1269
Size: 137 1132
BsiEI CG_ry'CG
Cuts at: 0 131 1269
Size: 131 1138
BsiHKAI G_wGCw'C
Cuts at: 0 667 1269
Size: 667 602
BsmI GAATG_Cn'
Cuts at: 0 1155 1269
Size: 1155 114
Bsp1286I G_dGCh'C
Cuts at: 0 667 1269
Size: 667 602
BspHI T'CATG_A
Cuts at: 0 293 1269
Size: 293 976
BsrBI CCG'CTC
Cuts at: 0 1212 1269
Size: 1212 57
BssHII G'CGCG_C
Cuts at: 0 36 1269
Size: 36 1233
BstXI CCAn_nnnn'nTGG
Cuts at: 0 104 1269
Size: 104 1165
BstYI r'GATC_y
Cuts at: 0 385 1269
Size: 385 884
Bsu36I CC'TnA_GG
Cuts at: 0 830 1269
Size: 830 439
BtgI C'CryG_G
Cuts at: 0 41 97 1269
Size: 41 56 1172
Fragments arranged by size:
1172 56 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 315 1269
Size: 315 954
BtsI GCAGTG_nn'
Cuts at: 0 790 1269
Size: 790 479
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1059 1094 1269
Size: 1059 35 175
Fragments arranged by size:
1059 175 35
EaeI y'GGCC_r
Cuts at: 0 128 1142 1269
Size: 128 1014 127
Fragments arranged by size:
1014 128 127
EagI C'GGCC_G
Cuts at: 0 128 1269
Size: 128 1141
EarI CTCTTCn'nnn_
Cuts at: 0 184 280 518 748 1092 1269
Size: 184 96 238 230 344 177
Fragments arranged by size:
344 238 230 184 177 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 398 1269
Size: 398 871
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 892 1269
Size: 102 790 377
Fragments arranged by size:
790 377 102
EcoO109I rG'GnC_Cy
Cuts at: 0 232 934 1137 1269
Size: 232 702 203 132
Fragments arranged by size:
702 232 203 132
HaeII r_GCGC'y
Cuts at: 0 845 1269
Size: 845 424
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 75 107 377 409 433 465 1228 1260
Size: 75 32 270 32 24 32 763 32
Cuts at: 1260 1269
Size: 9
Fragments arranged by size:
763 270 75 32 32 32 32 24
9
HincII GTy'rAC
Cuts at: 0 810 1269
Size: 810 459
MfeI C'AATT_G
Cuts at: 0 518 1269
Size: 518 751
MslI CAynn'nnrTG
Cuts at: 0 102 1269
Size: 102 1167
MspA1I CmG'CkG
Cuts at: 0 43 66 80 121 127 584 1194 1266
Size: 43 23 14 41 6 457 610 72
Cuts at: 1266 1269
Size: 3
Fragments arranged by size:
610 457 72 43 41 23 14 6
3
NcoI C'CATG_G
Cuts at: 0 97 1269
Size: 97 1172
NspI r_CATG'y
Cuts at: 0 157 1269
Size: 157 1112
PasI CC'CwG_GG
Cuts at: 0 159 1269
Size: 159 1110
PflMI CCAn_nnn'nTGG
Cuts at: 0 80 1269
Size: 80 1189
PpuMI rG'GwC_Cy
Cuts at: 0 934 1269
Size: 934 335
PstI C_TGCA'G
Cuts at: 0 126 1234 1269
Size: 126 1108 35
Fragments arranged by size:
1108 126 35
PvuII CAG'CTG
Cuts at: 0 80 121 584 1194 1266 1269
Size: 80 41 463 610 72 3
Fragments arranged by size:
610 463 80 72 41 3
SacII CC_GC'GG
Cuts at: 0 44 1269
Size: 44 1225
SapI GCTCTTCn'nnn_
Cuts at: 0 1092 1269
Size: 1092 177
ScaI AGT'ACT
Cuts at: 0 877 1054 1269
Size: 877 177 215
Fragments arranged by size:
877 215 177
SfcI C'TryA_G
Cuts at: 0 122 1230 1269
Size: 122 1108 39
Fragments arranged by size:
1108 122 39
SmlI C'TyrA_G
Cuts at: 0 173 748 1058 1269
Size: 173 575 310 211
Fragments arranged by size:
575 310 211 173
SphI G_CATG'C
Cuts at: 0 157 1269
Size: 157 1112
SspI AAT'ATT
Cuts at: 0 212 1269
Size: 212 1057
StyI C'CwwG_G
Cuts at: 0 97 908 1070 1269
Size: 97 811 162 199
Fragments arranged by size:
811 199 162 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 299 1269
Size: 299 970
TatI w'GTAC_w
Cuts at: 0 578 875 1052 1269
Size: 578 297 177 217
Fragments arranged by size:
578 297 217 177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 99 131 555 587 677 709 1269
Size: 99 32 424 32 90 32 560
Fragments arranged by size:
560 424 99 90 32 32 32
Enzymes that do cut:
AlwNI AseI BamHI BanI BciVI BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BsrBI BssHII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI
Eco57MI EcoO109I HaeII Hin4I HincII MfeI MslI MspA1I
NcoI NspI PasI PflMI PpuMI PstI PvuII SacII
SapI ScaI SfcI SmlI SphI SspI StyI TaqII
TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
ApoI AscI AsiSI AvaI AvrII BaeI BanII BbeI
BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BsaAI BsaBI BsaWI
BsiWI BsmBI BspEI BspMI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstZ17I ClaI DraI DraIII DrdI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI