Restriction map for
pF1KB3799
(Linear) (Six-base) MAPSORT of: KIBB3799.seq Check: 968 from: 1 to: 1455
KIBB3799.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 0 1160 1455
Size: 1160 295
AclI AA'CG_TT
Cuts at: 0 64 1455
Size: 64 1391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1204 1455
Size: 1204 251
AfeI AGC'GCT
Cuts at: 0 146 193 780 1455
Size: 146 47 587 675
Fragments arranged by size:
675 587 146 47
AflIII A'CryG_T
Cuts at: 0 47 452 1289 1455
Size: 47 405 837 166
Fragments arranged by size:
837 405 166 47
AleI CACnn'nnGTG
Cuts at: 0 650 829 1455
Size: 650 179 626
Fragments arranged by size:
650 626 179
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 124 424 1455
Size: 124 300 1031
Fragments arranged by size:
1031 300 124
AlwNI CAG_nnn'CTG
Cuts at: 0 111 162 183 1455
Size: 111 51 21 1272
Fragments arranged by size:
1272 111 51 21
ApaI G_GGCC'C
Cuts at: 0 203 306 315 1455
Size: 203 103 9 1140
Fragments arranged by size:
1140 203 103 9
ApaLI G'TGCA_C
Cuts at: 0 1297 1358 1455
Size: 1297 61 97
Fragments arranged by size:
1297 97 61
AvaI C'yCGr_G
Cuts at: 0 299 1100 1455
Size: 299 801 355
Fragments arranged by size:
801 355 299
BamHI G'GATC_C
Cuts at: 0 1104 1455
Size: 1104 351
BanI G'GyrC_C
Cuts at: 0 134 320 408 606 1455
Size: 134 186 88 198 849
Fragments arranged by size:
849 198 186 134 88
BanII G_rGCy'C
Cuts at: 0 203 306 315 898 1057 1213 1455
Size: 203 103 9 583 159 156 242
Fragments arranged by size:
583 242 203 159 156 103 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 975 1455
Size: 975 480
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 225 259 454 488 1413 1447 1455
Size: 225 34 195 34 925 34 8
Fragments arranged by size:
925 225 195 34 34 34 8
BglI GCCn_nnn'nGGC
Cuts at: 0 310 1455
Size: 310 1145
Bme1580I G_kGCm'C
Cuts at: 0 137 203 306 315 323 413 1301 1362
Size: 137 66 103 9 8 90 888 61
Cuts at: 1362 1455
Size: 93
Fragments arranged by size:
888 137 103 93 90 66 61 9
8
BmgBI CAC'GTC
Cuts at: 0 416 455 1455
Size: 416 39 1000
Fragments arranged by size:
1000 416 39
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1227 1455
Size: 1227 228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 882 1392 1455
Size: 882 510 63
Fragments arranged by size:
882 510 63
BsaAI yAC'GTr
Cuts at: 0 510 1455
Size: 510 945
BsaHI Gr'CG_yC
Cuts at: 0 1415 1455
Size: 1415 40
BseYI C'CCAG_C
Cuts at: 0 130 141 183 1455
Size: 130 11 42 1272
Fragments arranged by size:
1272 130 42 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 1455
Size: 780 675
BsiHKAI G_wGCw'C
Cuts at: 0 225 1213 1301 1362 1455
Size: 225 988 88 61 93
Fragments arranged by size:
988 225 93 88 61
BsmI GAATG_Cn'
Cuts at: 0 126 1455
Size: 126 1329
Bsp1286I G_dGCh'C
Cuts at: 0 137 203 225 306 315 323 413 898
Size: 137 66 22 81 9 8 90 485
Cuts at: 898 1057 1213 1301 1362 1455
Size: 159 156 88 61 93
Fragments arranged by size:
485 159 156 137 93 90 88 81
66 61 22 9 8
BspHI T'CATG_A
Cuts at: 0 860 1455
Size: 860 595
BsrBI CCG'CTC
Cuts at: 0 1317 1455
Size: 1317 138
BsrFI r'CCGG_y
Cuts at: 0 95 947 1455
Size: 95 852 508
Fragments arranged by size:
852 508 95
BssSI C'ACGA_G
Cuts at: 0 224 694 1455
Size: 224 470 761
Fragments arranged by size:
761 470 224
BstAPI GCAn_nnn'nTGC
Cuts at: 0 852 1455
Size: 852 603
BstXI CCAn_nnnn'nTGG
Cuts at: 0 652 708 1035 1425 1455
Size: 652 56 327 390 30
Fragments arranged by size:
652 390 327 56 30
BstYI r'GATC_y
Cuts at: 0 761 1104 1455
Size: 761 343 351
Fragments arranged by size:
761 351 343
Bsu36I CC'TnA_GG
Cuts at: 0 163 1455
Size: 163 1292
BtgI C'CryG_G
Cuts at: 0 5 315 440 651 972 1455
Size: 5 310 125 211 321 483
Fragments arranged by size:
483 321 310 211 125 5
BtsI GCAGTG_nn'
Cuts at: 0 35 225 984 1111 1287 1455
Size: 35 190 759 127 176 168
Fragments arranged by size:
759 190 176 168 127 35
DraIII CAC_nnn'GTG
Cuts at: 0 852 1455
Size: 852 603
EaeI y'GGCC_r
Cuts at: 0 2 443 945 1004 1322 1455
Size: 2 441 502 59 318 133
Fragments arranged by size:
502 441 318 133 59 2
EarI CTCTTCn'nnn_
Cuts at: 0 1180 1455
Size: 1180 275
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 82 1243 1455
Size: 82 1161 212
Fragments arranged by size:
1161 212 82
EcoICRI GAG'CTC
Cuts at: 0 1211 1455
Size: 1211 244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 882 1204 1392 1455
Size: 882 322 188 63
Fragments arranged by size:
882 322 188 63
EcoO109I rG'GnC_Cy
Cuts at: 0 200 303 311 392 1172 1262 1455
Size: 200 103 8 81 780 90 193
Fragments arranged by size:
780 200 193 103 90 81 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1209 1455
Size: 1209 246
FspI TGC'GCA
Cuts at: 0 724 1225 1455
Size: 724 501 230
Fragments arranged by size:
724 501 230
FspAI rTGC'GCAy
Cuts at: 0 724 1455
Size: 724 731
HaeII r_GCGC'y
Cuts at: 0 148 195 782 1455
Size: 148 47 587 673
Fragments arranged by size:
673 587 148 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1200 1232 1455
Size: 1200 32 223
Fragments arranged by size:
1200 223 32
HincII GTy'rAC
Cuts at: 0 813 1338 1455
Size: 813 525 117
Fragments arranged by size:
813 525 117
HindIII A'AGCT_T
Cuts at: 0 1191 1455
Size: 1191 264
MluI A'CGCG_T
Cuts at: 0 47 1455
Size: 47 1408
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 1455
Size: 686 769
MscI TGG'CCA
Cuts at: 0 1006 1455
Size: 1006 449
MslI CAynn'nnrTG
Cuts at: 0 512 650 694 829 1294 1455
Size: 512 138 44 135 465 161
Fragments arranged by size:
512 465 161 138 135 44
MspA1I CmG'CkG
Cuts at: 0 7 108 180 354 603 1182 1421 1455
Size: 7 101 72 174 249 579 239 34
Fragments arranged by size:
579 249 239 174 101 72 34 7
NaeI GCC'GGC
Cuts at: 0 97 949 1455
Size: 97 852 506
Fragments arranged by size:
852 506 97
NcoI C'CATG_G
Cuts at: 0 440 1455
Size: 440 1015
NgoMIV G'CCGG_C
Cuts at: 0 95 947 1455
Size: 95 852 508
Fragments arranged by size:
852 508 95
NspI r_CATG'y
Cuts at: 0 489 859 1293 1455
Size: 489 370 434 162
Fragments arranged by size:
489 434 370 162
PasI CC'CwG_GG
Cuts at: 0 306 1455
Size: 306 1149
PciI A'CATG_T
Cuts at: 0 1289 1455
Size: 1289 166
PflMI CCAn_nnn'nTGG
Cuts at: 0 510 1455
Size: 510 945
PmlI CAC'GTG
Cuts at: 0 510 1455
Size: 510 945
PpuMI rG'GwC_Cy
Cuts at: 0 1172 1455
Size: 1172 283
PspOMI G'GGCC_C
Cuts at: 0 199 302 311 1455
Size: 199 103 9 1144
Fragments arranged by size:
1144 199 103 9
PstI C_TGCA'G
Cuts at: 0 359 1181 1349 1455
Size: 359 822 168 106
Fragments arranged by size:
822 359 168 106
PvuII CAG'CTG
Cuts at: 0 108 180 354 603 1182 1455
Size: 108 72 174 249 579 273
Fragments arranged by size:
579 273 249 174 108 72
SacI G_AGCT'C
Cuts at: 0 1213 1455
Size: 1213 242
SacII CC_GC'GG
Cuts at: 0 8 1455
Size: 8 1447
SanDI GG'GwC_CC
Cuts at: 0 1172 1455
Size: 1172 283
SfcI C'TryA_G
Cuts at: 0 231 355 1149 1177 1345 1455
Size: 231 124 794 28 168 110
Fragments arranged by size:
794 231 168 124 110 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 310 1455
Size: 310 1145
SgrAI Cr'CCGG_yG
Cuts at: 0 95 1455
Size: 95 1360
SmaI CCC'GGG
Cuts at: 0 1102 1455
Size: 1102 353
StyI C'CwwG_G
Cuts at: 0 125 440 526 1325 1455
Size: 125 315 86 799 130
Fragments arranged by size:
799 315 130 125 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 566 633 1455
Size: 566 67 822
Fragments arranged by size:
822 566 67
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1035 1455
Size: 1035 420
XmaI C'CCGG_G
Cuts at: 0 1100 1455
Size: 1100 355
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII AleI AlfI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbsI BcgI
BglI Bme1580I BmgBI BplI BpmI BsaAI BsaHI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrFI BssSI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI FspAI
HaeII Hin4I HincII HindIII MluI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI
PmlI PpuMI PspOMI PstI PvuII SacI SacII SanDI
SfcI SfiI SgrAI SmaI StyI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AflII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BciVI
BclI BfrBI BglII BlpI BmrI BmtI Bpu10I BpuEI
BsaI BsaBI BsaWI BsaXI BseRI BsiEI BsiWI BsmBI
BspEI BspMI BsrDI BsrGI BssHII BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EcoNI EcoRI
EcoRV FseI HpaI KasI KpnI MfeI NarI NdeI
NheI NotI NruI NsiI PacI PfoI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI
SbfI ScaI SexAI SfoI SgfI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmnI ZraI