Restriction map for
pF1KB3774
(Linear) (Six-base) MAPSORT of: KIBB3774.seq Check: 4154 from: 1 to: 1050
KIBB3774.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 0 323 1050
Size: 323 727
AccI GT'mk_AC
Cuts at: 0 1037 1050
Size: 1037 13
Acc65I G'GTAC_C
Cuts at: 0 824 1050
Size: 824 226
AflIII A'CryG_T
Cuts at: 0 884 1050
Size: 884 166
AgeI A'CCGG_T
Cuts at: 0 941 1050
Size: 941 109
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 376 408 1050
Size: 376 32 642
Fragments arranged by size:
642 376 32
ApaI G_GGCC'C
Cuts at: 0 123 1050
Size: 123 927
ApaLI G'TGCA_C
Cuts at: 0 973 1050
Size: 973 77
AvaI C'yCGr_G
Cuts at: 0 195 293 471 1050
Size: 195 98 178 579
Fragments arranged by size:
579 195 178 98
BanI G'GyrC_C
Cuts at: 0 150 238 543 648 824 920 1050
Size: 150 88 305 105 176 96 130
Fragments arranged by size:
305 176 150 130 105 96 88
BanII G_rGCy'C
Cuts at: 0 123 490 1050
Size: 123 367 560
Fragments arranged by size:
560 367 123
BbeI G_GCGC'C
Cuts at: 0 547 1050
Size: 547 503
BbsI GAAGACnn'nnnn_
Cuts at: 0 970 1050
Size: 970 80
BbvCI CC'TCA_GC
Cuts at: 0 178 1050
Size: 178 872
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 220 254 1050
Size: 220 34 796
Fragments arranged by size:
796 220 34
BglII A'GATC_T
Cuts at: 0 764 1050
Size: 764 286
Bme1580I G_kGCm'C
Cuts at: 0 123 155 518 653 977 1050
Size: 123 32 363 135 324 73
Fragments arranged by size:
363 324 135 123 73 32
BmgBI CAC'GTC
Cuts at: 0 507 1050
Size: 507 543
BmrI ACTGGGnnnn_n'
Cuts at: 0 345 718 939 1050
Size: 345 373 221 111
Fragments arranged by size:
373 345 221 111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 210 1050
Size: 210 840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1050
Size: 403 647
Bpu10I CC'TnA_GC
Cuts at: 0 178 1050
Size: 178 872
BsaHI Gr'CG_yC
Cuts at: 0 320 544 1050
Size: 320 224 506
Fragments arranged by size:
506 320 224
BsaWI w'CCGG_w
Cuts at: 0 315 941 959 1050
Size: 315 626 18 91
Fragments arranged by size:
626 315 91 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 376 406 1050
Size: 376 30 644
Fragments arranged by size:
644 376 30
BseYI C'CCAG_C
Cuts at: 0 774 1050
Size: 774 276
BsiHKAI G_wGCw'C
Cuts at: 0 10 977 1050
Size: 10 967 73
Fragments arranged by size:
967 73 10
Bsp1286I G_dGCh'C
Cuts at: 0 10 123 155 490 518 653 977 1050
Size: 10 113 32 335 28 135 324 73
Fragments arranged by size:
335 324 135 113 73 32 28 10
BspEI T'CCGG_A
Cuts at: 0 959 1050
Size: 959 91
BspHI T'CATG_A
Cuts at: 0 527 1050
Size: 527 523
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 488 629 1050
Size: 488 141 421
Fragments arranged by size:
488 421 141
BsrFI r'CCGG_y
Cuts at: 0 163 235 446 941 1050
Size: 163 72 211 495 109
Fragments arranged by size:
495 211 163 109 72
BstYI r'GATC_y
Cuts at: 0 306 704 764 1050
Size: 306 398 60 286
Fragments arranged by size:
398 306 286 60
BtgI C'CryG_G
Cuts at: 0 32 458 551 695 833 1050
Size: 32 426 93 144 138 217
Fragments arranged by size:
426 217 144 138 93 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 245 1050
Size: 245 805
DraIII CAC_nnn'GTG
Cuts at: 0 141 1050
Size: 141 909
EaeI y'GGCC_r
Cuts at: 0 92 1013 1050
Size: 92 921 37
Fragments arranged by size:
921 92 37
EarI CTCTTCn'nnn_
Cuts at: 0 73 1050
Size: 73 977
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1050
Size: 403 647
EcoNI CCTnn'n_nnAGG
Cuts at: 0 181 1050
Size: 181 869
EcoO109I rG'GnC_Cy
Cuts at: 0 119 120 432 660 1050
Size: 119 1 312 228 390
Fragments arranged by size:
390 312 228 119 1
HaeII r_GCGC'y
Cuts at: 0 177 453 547 689 1050
Size: 177 276 94 142 361
Fragments arranged by size:
361 276 177 142 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 522 554 798 830 1050
Size: 522 32 244 32 220
Fragments arranged by size:
522 244 220 32 32
KasI G'GCGC_C
Cuts at: 0 543 1050
Size: 543 507
KpnI G_GTAC'C
Cuts at: 0 828 1050
Size: 828 222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 1050
Size: 269 781
MscI TGG'CCA
Cuts at: 0 94 1015 1050
Size: 94 921 35
Fragments arranged by size:
921 94 35
MslI CAynn'nnrTG
Cuts at: 0 532 838 869 1006 1050
Size: 532 306 31 137 44
Fragments arranged by size:
532 306 137 44 31
MspA1I CmG'CkG
Cuts at: 0 221 1050
Size: 221 829
NaeI GCC'GGC
Cuts at: 0 165 448 1050
Size: 165 283 602
Fragments arranged by size:
602 283 165
NarI GG'CG_CC
Cuts at: 0 544 1050
Size: 544 506
NgoMIV G'CCGG_C
Cuts at: 0 163 446 1050
Size: 163 283 604
Fragments arranged by size:
604 283 163
NspI r_CATG'y
Cuts at: 0 868 1011 1050
Size: 868 143 39
Fragments arranged by size:
868 143 39
PflMI CCAn_nnn'nTGG
Cuts at: 0 90 1050
Size: 90 960
PfoI T'CCnGG_A
Cuts at: 0 428 662 1050
Size: 428 234 388
Fragments arranged by size:
428 388 234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 1050
Size: 376 32 642
Fragments arranged by size:
642 376 32
PpuMI rG'GwC_Cy
Cuts at: 0 432 660 1050
Size: 432 228 390
Fragments arranged by size:
432 390 228
PspOMI G'GGCC_C
Cuts at: 0 119 1050
Size: 119 931
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 928 960 1050
Size: 928 32 90
Fragments arranged by size:
928 90 32
PstI C_TGCA'G
Cuts at: 0 173 347 1050
Size: 173 174 703
Fragments arranged by size:
703 174 173
SanDI GG'GwC_CC
Cuts at: 0 432 1050
Size: 432 618
SapI GCTCTTCn'nnn_
Cuts at: 0 73 1050
Size: 73 977
SfcI C'TryA_G
Cuts at: 0 169 343 1050
Size: 169 174 707
Fragments arranged by size:
707 174 169
SfoI GGC'GCC
Cuts at: 0 545 1050
Size: 545 505
StyI C'CwwG_G
Cuts at: 0 1016 1050
Size: 1016 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 601 1050
Size: 601 449
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 305 337 658 690 1050
Size: 305 32 321 32 360
Fragments arranged by size:
360 321 305 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 378 403 840 1050
Size: 378 25 437 210
Fragments arranged by size:
437 378 210 25
XmnI GAAnn'nnTTC
Cuts at: 0 391 733 1050
Size: 391 342 317
Fragments arranged by size:
391 342 317
ZraI GAC'GTC
Cuts at: 0 321 1050
Size: 321 729
Enzymes that do cut:
AatII AccI Acc65I AflIII AgeI AloI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaHI BsaWI
BsaXI BseYI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrFI
BstYI BtgI BtgZI DraIII EaeI EarI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PflMI PfoI PpiI
PpuMI PspOMI PsrI PstI SanDI SapI SfcI SfoI
StyI TaqII TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AclI AcuI AfeI AflII AhdI AleI AlfI
AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BglI BlpI BmtI BpuEI BsaI
BsaAI BsaBI BseRI BsgI BsiEI BsiWI BsmI BsmBI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI MfeI MluI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PshAI PsiI PspXI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XhoI XmaI