Restriction map for
pF1KB3765
(Linear) (Six-base) MAPSORT of: KIBB3765.seq Check: 1032 from: 1 to: 1401
KIBB3765.orf
With 182 enzymes: SgfI *
April 12, 2013 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 171 1401
Size: 171 1230
AclI AA'CG_TT
Cuts at: 0 569 1401
Size: 569 832
ApoI r'AATT_y
Cuts at: 0 609 933 1401
Size: 609 324 468
Fragments arranged by size:
609 468 324
AseI AT'TA_AT
Cuts at: 0 1243 1401
Size: 1243 158
BanI G'GyrC_C
Cuts at: 0 232 1401
Size: 232 1169
BanII G_rGCy'C
Cuts at: 0 387 1401
Size: 387 1014
BbeI G_GCGC'C
Cuts at: 0 236 1401
Size: 236 1165
BbsI GAAGACnn'nnnn_
Cuts at: 0 1121 1401
Size: 1121 280
BbvCI CC'TCA_GC
Cuts at: 0 1188 1401
Size: 1188 213
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1086 1120 1401
Size: 1086 34 281
Fragments arranged by size:
1086 281 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 936 1111 1401
Size: 936 175 290
Fragments arranged by size:
936 290 175
BclI T'GATC_A
Cuts at: 0 245 416 1401
Size: 245 171 985
Fragments arranged by size:
985 245 171
BglI GCCn_nnn'nGGC
Cuts at: 0 697 1401
Size: 697 704
Bme1580I G_kGCm'C
Cuts at: 0 693 1401
Size: 693 708
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1401
Size: 646 755
Bpu10I CC'TnA_GC
Cuts at: 0 64 140 824 1188 1401
Size: 64 76 684 364 213
Fragments arranged by size:
684 364 213 76 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 896 1401
Size: 896 505
BsaAI yAC'GTr
Cuts at: 0 79 1401
Size: 79 1322
BsaHI Gr'CG_yC
Cuts at: 0 233 1401
Size: 233 1168
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 644 674 1401
Size: 644 30 727
Fragments arranged by size:
727 644 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 855 1401
Size: 226 629 546
Fragments arranged by size:
629 546 226
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1401
Size: 373 1028
BsiHKAI G_wGCw'C
Cuts at: 0 113 1401
Size: 113 1288
BsmI GAATG_Cn'
Cuts at: 0 42 408 1381 1401
Size: 42 366 973 20
Fragments arranged by size:
973 366 42 20
Bsp1286I G_dGCh'C
Cuts at: 0 113 387 693 1401
Size: 113 274 306 708
Fragments arranged by size:
708 306 274 113
BspHI T'CATG_A
Cuts at: 0 413 434 1401
Size: 413 21 967
Fragments arranged by size:
967 413 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 21 171 1401
Size: 21 150 1230
Fragments arranged by size:
1230 150 21
BsrDI GCAATG_nn'
Cuts at: 0 1291 1401
Size: 1291 110
BsrGI T'GTAC_A
Cuts at: 0 1356 1401
Size: 1356 45
BssSI C'ACGA_G
Cuts at: 0 1070 1401
Size: 1070 331
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1036 1401
Size: 1036 365
DraI TTT'AAA
Cuts at: 0 558 1401
Size: 558 843
EaeI y'GGCC_r
Cuts at: 0 132 1401
Size: 132 1269
EarI CTCTTCn'nnn_
Cuts at: 0 1307 1401
Size: 1307 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 1401
Size: 646 755
EcoO109I rG'GnC_Cy
Cuts at: 0 1045 1401
Size: 1045 356
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 670 817 1401
Size: 670 147 584
Fragments arranged by size:
670 584 147
HaeII r_GCGC'y
Cuts at: 0 236 1401
Size: 236 1165
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 1020 1052 1064 1096 1401
Size: 133 32 855 32 12 32 305
Fragments arranged by size:
855 305 133 32 32 32 12
KasI G'GCGC_C
Cuts at: 0 232 1401
Size: 232 1169
MscI TGG'CCA
Cuts at: 0 134 1401
Size: 134 1267
MslI CAynn'nnrTG
Cuts at: 0 280 1401
Size: 280 1121
NarI GG'CG_CC
Cuts at: 0 233 1401
Size: 233 1168
NspI r_CATG'y
Cuts at: 0 244 1401
Size: 244 1157
PasI CC'CwG_GG
Cuts at: 0 165 1401
Size: 165 1236
PflMI CCAn_nnn'nTGG
Cuts at: 0 641 1401
Size: 641 760
PfoI T'CCnGG_A
Cuts at: 0 1082 1401
Size: 1082 319
SapI GCTCTTCn'nnn_
Cuts at: 0 1307 1401
Size: 1307 94
SfcI C'TryA_G
Cuts at: 0 1164 1401
Size: 1164 237
SfoI GGC'GCC
Cuts at: 0 234 1401
Size: 234 1167
SnaBI TAC'GTA
Cuts at: 0 79 1401
Size: 79 1322
StyI C'CwwG_G
Cuts at: 0 129 1401
Size: 129 1272
TatI w'GTAC_w
Cuts at: 0 1356 1401
Size: 1356 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 600 632 644 676 1401
Size: 600 32 12 32 725
Fragments arranged by size:
725 600 32 32 12
XmnI GAAnn'nnTTC
Cuts at: 0 630 1401
Size: 630 771
Enzymes that do cut:
AarI AclI ApoI AseI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI Bme1580I BpmI Bpu10I
BsaI BsaAI BsaHI BsaXI BseRI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BtgZI DraI
EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I KasI
MscI MslI NarI NspI PasI PflMI PfoI SapI
SfcI SfoI SnaBI StyI TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI BpuEI BsaBI BsaWI BseYI
BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XcmI XhoI XmaI ZraI