Restriction map for
pF1KB3761
(Linear) (Six-base) MAPSORT of: KIBB3761.seq Check: 3426 from: 1 to: 3204
KIBB3761.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AccI GT'mk_AC
Cuts at: 0 394 3204
Size: 394 2810
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 3204
Size: 746 2458
AfeI AGC'GCT
Cuts at: 0 3000 3204
Size: 3000 204
AflIII A'CryG_T
Cuts at: 0 329 1460 2464 3132 3204
Size: 329 1131 1004 668 72
Fragments arranged by size:
1131 1004 668 329 72
AhdI GACnn_n'nnGTC
Cuts at: 0 1313 3204
Size: 1313 1891
AlwNI CAG_nnn'CTG
Cuts at: 0 69 81 3204
Size: 69 12 3123
Fragments arranged by size:
3123 69 12
ApoI r'AATT_y
Cuts at: 0 349 381 1394 2473 2794 3204
Size: 349 32 1013 1079 321 410
Fragments arranged by size:
1079 1013 410 349 321 32
AseI AT'TA_AT
Cuts at: 0 2272 3204
Size: 2272 932
BanII G_rGCy'C
Cuts at: 0 1640 3204
Size: 1640 1564
BbsI GAAGACnn'nnnn_
Cuts at: 0 1449 1885 1980 3204
Size: 1449 436 95 1224
Fragments arranged by size:
1449 1224 436 95
BbvCI CC'TCA_GC
Cuts at: 0 23 3204
Size: 23 3181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 109 143 3094 3128 3204
Size: 109 34 2951 34 76
Fragments arranged by size:
2951 109 76 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 55 3204
Size: 55 3149
Bme1580I G_kGCm'C
Cuts at: 0 649 3204
Size: 649 2555
BmgBI CAC'GTC
Cuts at: 0 2465 3204
Size: 2465 739
BmrI ACTGGGnnnn_n'
Cuts at: 0 2119 2363 3204
Size: 2119 244 841
Fragments arranged by size:
2119 841 244
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 880 1656 3204
Size: 880 776 1548
Fragments arranged by size:
1548 880 776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1717 2207 3204
Size: 1717 490 997
Fragments arranged by size:
1717 997 490
Bpu10I CC'TnA_GC
Cuts at: 0 23 2361 3204
Size: 23 2338 843
Fragments arranged by size:
2338 843 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1626 3204
Size: 1626 1578
BsaAI yAC'GTr
Cuts at: 0 2410 2631 3204
Size: 2410 221 573
Fragments arranged by size:
2410 573 221
BsaWI w'CCGG_w
Cuts at: 0 1114 3204
Size: 1114 2090
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1796 1826 3204
Size: 1796 30 1378
Fragments arranged by size:
1796 1378 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 504 1835 2518 3204
Size: 504 1331 683 686
Fragments arranged by size:
1331 686 683 504
BseYI C'CCAG_C
Cuts at: 0 2939 3204
Size: 2939 265
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1700 1834 3204
Size: 1700 134 1370
Fragments arranged by size:
1700 1370 134
BsiEI CG_ry'CG
Cuts at: 0 107 3204
Size: 107 3097
BsiHKAI G_wGCw'C
Cuts at: 0 806 1529 3204
Size: 806 723 1675
Fragments arranged by size:
1675 806 723
BsmI GAATG_Cn'
Cuts at: 0 865 3204
Size: 865 2339
Bsp1286I G_dGCh'C
Cuts at: 0 649 806 1529 1640 3204
Size: 649 157 723 111 1564
Fragments arranged by size:
1564 723 649 157 111
BsrBI CCG'CTC
Cuts at: 0 1625 3204
Size: 1625 1579
BsrDI GCAATG_nn'
Cuts at: 0 743 3204
Size: 743 2461
BsrFI r'CCGG_y
Cuts at: 0 1184 3204
Size: 1184 2020
BsrGI T'GTAC_A
Cuts at: 0 3008 3204
Size: 3008 196
BssSI C'ACGA_G
Cuts at: 0 1010 3204
Size: 1010 2194
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1963 2182 3204
Size: 1963 219 1022
Fragments arranged by size:
1963 1022 219
BstBI TT'CG_AA
Cuts at: 0 1875 1896 3204
Size: 1875 21 1308
Fragments arranged by size:
1875 1308 21
BstYI r'GATC_y
Cuts at: 0 513 618 956 1351 3204
Size: 513 105 338 395 1853
Fragments arranged by size:
1853 513 395 338 105
BtgI C'CryG_G
Cuts at: 0 218 3204
Size: 218 2986
BtsI GCAGTG_nn'
Cuts at: 0 510 908 3204
Size: 510 398 2296
Fragments arranged by size:
2296 510 398
ClaI AT'CG_AT
Cuts at: 0 3104 3204
Size: 3104 100
DraI TTT'AAA
Cuts at: 0 681 3204
Size: 681 2523
DraIII CAC_nnn'GTG
Cuts at: 0 1225 3204
Size: 1225 1979
EaeI y'GGCC_r
Cuts at: 0 104 2114 3204
Size: 104 2010 1090
Fragments arranged by size:
2010 1090 104
EagI C'GGCC_G
Cuts at: 0 104 3204
Size: 104 3100
EarI CTCTTCn'nnn_
Cuts at: 0 722 914 974 1625 1893 2108 2814 3204
Size: 722 192 60 651 268 215 706 390
Fragments arranged by size:
722 706 651 390 268 215 192 60
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 1717 2207 3204
Size: 746 971 490 997
Fragments arranged by size:
997 971 746 490
EcoNI CCTnn'n_nnAGG
Cuts at: 0 73 91 1955 3204
Size: 73 18 1864 1249
Fragments arranged by size:
1864 1249 73 18
EcoO109I rG'GnC_Cy
Cuts at: 0 66 1989 2357 3204
Size: 66 1923 368 847
Fragments arranged by size:
1923 847 368 66
EcoRI G'AATT_C
Cuts at: 0 2473 3204
Size: 2473 731
EcoRV GAT'ATC
Cuts at: 0 3048 3204
Size: 3048 156
HaeII r_GCGC'y
Cuts at: 0 61 3002 3204
Size: 61 2941 202
Fragments arranged by size:
2941 202 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1299 1331 2862 2894 3103 3135 3204
Size: 1299 32 1531 32 209 32 69
Fragments arranged by size:
1531 1299 209 69 32 32 32
HincII GTy'rAC
Cuts at: 0 1318 3204
Size: 1318 1886
MluI A'CGCG_T
Cuts at: 0 3132 3204
Size: 3132 72
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 511 3204
Size: 204 307 2693
Fragments arranged by size:
2693 307 204
MscI TGG'CCA
Cuts at: 0 2116 3204
Size: 2116 1088
MslI CAynn'nnrTG
Cuts at: 0 2972 3204
Size: 2972 232
NdeI CA'TA_TG
Cuts at: 0 450 2867 3204
Size: 450 2417 337
Fragments arranged by size:
2417 450 337
NspI r_CATG'y
Cuts at: 0 182 200 333 1464 3204
Size: 182 18 133 1131 1740
Fragments arranged by size:
1740 1131 182 133 18
PciI A'CATG_T
Cuts at: 0 329 1460 3204
Size: 329 1131 1744
Fragments arranged by size:
1744 1131 329
PflMI CCAn_nnn'nTGG
Cuts at: 0 2167 2871 3204
Size: 2167 704 333
Fragments arranged by size:
2167 704 333
PfoI T'CCnGG_A
Cuts at: 0 866 3204
Size: 866 2338
PpuMI rG'GwC_Cy
Cuts at: 0 1989 2357 3204
Size: 1989 368 847
Fragments arranged by size:
1989 847 368
PsiI TTA'TAA
Cuts at: 0 1237 1406 1601 2791 3204
Size: 1237 169 195 1190 413
Fragments arranged by size:
1237 1190 413 195 169
SapI GCTCTTCn'nnn_
Cuts at: 0 722 3204
Size: 722 2482
SexAI A'CCwGG_T
Cuts at: 0 2091 3204
Size: 2091 1113
SfcI C'TryA_G
Cuts at: 0 61 144 1536 1941 3172 3204
Size: 61 83 1392 405 1231 32
Fragments arranged by size:
1392 1231 405 83 61 32
SmlI C'TyrA_G
Cuts at: 0 1641 3204
Size: 1641 1563
SphI G_CATG'C
Cuts at: 0 200 3204
Size: 200 3004
SspI AAT'ATT
Cuts at: 0 404 1242 3204
Size: 404 838 1962
Fragments arranged by size:
1962 838 404
StuI AGG'CCT
Cuts at: 0 2984 3204
Size: 2984 220
StyI C'CwwG_G
Cuts at: 0 1558 3204
Size: 1558 1646
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1186 1871 3204
Size: 1186 685 1333
Fragments arranged by size:
1333 1186 685
TatI w'GTAC_w
Cuts at: 0 750 3008 3204
Size: 750 2258 196
Fragments arranged by size:
2258 750 196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1796 1828 3204
Size: 1796 32 1376
Fragments arranged by size:
1796 1376 32
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AlwNI ApoI AseI
BanII BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtsI
ClaI DraI DraIII EaeI EagI EarI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I HincII MluI MmeI
MscI MslI NdeI NspI PciI PflMI PfoI PpuMI
PsiI SapI SexAI SfcI SmlI SphI SspI StuI
StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BciVI BclI BfrBI BglII BlpI
BmtI BsaI BsaBI BsaHI BsiWI BsmBI BspEI BspHI
BspMI BssHII BstEII BstXI BstZ17I Bsu36I BtgZI CspCI
DrdI EciI EcoICRI FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MspA1I NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI