Restriction map for
pF1KB3746
(Linear) (Six-base) MAPSORT of: KIBB3746.seq Check: 8325 from: 1 to: 1026
KIBB3746.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 860 1026
Size: 545 315 166
Fragments arranged by size:
545 315 166
AlwNI CAG_nnn'CTG
Cuts at: 0 419 945 1026
Size: 419 526 81
Fragments arranged by size:
526 419 81
ApaI G_GGCC'C
Cuts at: 0 285 1026
Size: 285 741
AvaI C'yCGr_G
Cuts at: 0 128 952 1026
Size: 128 824 74
Fragments arranged by size:
824 128 74
BanII G_rGCy'C
Cuts at: 0 261 285 1026
Size: 261 24 741
Fragments arranged by size:
741 261 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 116 327 887 1026
Size: 116 211 560 139
Fragments arranged by size:
560 211 139 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 163 197 207 241 1026
Size: 163 34 10 34 785
Fragments arranged by size:
785 163 34 34 10
BciVI GTATCCnnnnn_n'
Cuts at: 0 446 1026
Size: 446 580
Bme1580I G_kGCm'C
Cuts at: 0 285 829 1026
Size: 285 544 197
Fragments arranged by size:
544 285 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1026
Size: 619 407
Bpu10I CC'TnA_GC
Cuts at: 0 77 214 1026
Size: 77 137 812
Fragments arranged by size:
812 137 77
BsaBI GATnn'nnATC
Cuts at: 0 628 1026
Size: 628 398
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 1026
Size: 283 743
BseYI C'CCAG_C
Cuts at: 0 832 1026
Size: 832 194
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1026
Size: 193 833
BsiEI CG_ry'CG
Cuts at: 0 279 1026
Size: 279 747
BsiHKAI G_wGCw'C
Cuts at: 0 100 220 1026
Size: 100 120 806
Fragments arranged by size:
806 120 100
Bsp1286I G_dGCh'C
Cuts at: 0 100 220 261 285 829 1026
Size: 100 120 41 24 544 197
Fragments arranged by size:
544 197 120 100 41 24
BstEII G'GTnAC_C
Cuts at: 0 117 1026
Size: 117 909
BstXI CCAn_nnnn'nTGG
Cuts at: 0 449 541 1026
Size: 449 92 485
Fragments arranged by size:
485 449 92
BtgI C'CryG_G
Cuts at: 0 759 1026
Size: 759 267
BtsI GCAGTG_nn'
Cuts at: 0 845 1026
Size: 845 181
DraI TTT'AAA
Cuts at: 0 696 1026
Size: 696 330
DraIII CAC_nnn'GTG
Cuts at: 0 979 1026
Size: 979 47
EarI CTCTTCn'nnn_
Cuts at: 0 128 1026
Size: 128 898
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 427 1026
Size: 427 599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 619 860 1026
Size: 545 74 241 166
Fragments arranged by size:
545 241 166 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 811 1026
Size: 811 215
EcoO109I rG'GnC_Cy
Cuts at: 0 177 282 957 1026
Size: 177 105 675 69
Fragments arranged by size:
675 177 105 69
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 388 1026
Size: 388 638
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 740 760 772 792 1026
Size: 740 20 12 20 234
Fragments arranged by size:
740 234 20 20 12
HincII GTy'rAC
Cuts at: 0 300 1026
Size: 300 726
MspA1I CmG'CkG
Cuts at: 0 311 1011 1026
Size: 311 700 15
Fragments arranged by size:
700 311 15
NcoI C'CATG_G
Cuts at: 0 759 1026
Size: 759 267
PasI CC'CwG_GG
Cuts at: 0 413 1026
Size: 413 613
PfoI T'CCnGG_A
Cuts at: 0 635 1026
Size: 635 391
PspOMI G'GGCC_C
Cuts at: 0 281 1026
Size: 281 745
PstI C_TGCA'G
Cuts at: 0 143 290 1026
Size: 143 147 736
Fragments arranged by size:
736 147 143
PvuII CAG'CTG
Cuts at: 0 311 1011 1026
Size: 311 700 15
Fragments arranged by size:
700 311 15
SapI GCTCTTCn'nnn_
Cuts at: 0 128 1026
Size: 128 898
SexAI A'CCwGG_T
Cuts at: 0 113 1026
Size: 113 913
SfcI C'TryA_G
Cuts at: 0 139 286 312 1026
Size: 139 147 26 714
Fragments arranged by size:
714 147 139 26
SmaI CCC'GGG
Cuts at: 0 130 1026
Size: 130 896
SspI AAT'ATT
Cuts at: 0 692 1026
Size: 692 334
StyI C'CwwG_G
Cuts at: 0 759 812 1026
Size: 759 53 214
Fragments arranged by size:
759 214 53
TatI w'GTAC_w
Cuts at: 0 1013 1026
Size: 1013 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 777 809 1026
Size: 777 32 217
Fragments arranged by size:
777 217 32
Tth111I GACn'n_nGTC
Cuts at: 0 115 1026
Size: 115 911
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 36 1026
Size: 36 990
XmaI C'CCGG_G
Cuts at: 0 128 1026
Size: 128 898
XmnI GAAnn'nnTTC
Cuts at: 0 898 1026
Size: 898 128
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanII BbsI BcgI BciVI
Bme1580I BpmI Bpu10I BsaBI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BstEII BstXI BtgI BtsI DraI DraIII
EarI EciI Eco57MI EcoNI EcoO109I FalI Hin4I HincII
MspA1I NcoI PasI PfoI PspOMI PstI PvuII SapI
SexAI SfcI SmaI SspI StyI TatI TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DrdI EaeI EagI EcoICRI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI StuI SwaI TaqII XbaI XhoI
ZraI