Restriction map for
pF1KB3737
(Linear) (Six-base) MAPSORT of: KIBB3737.seq Check: 4728 from: 1 to: 1251
KIBB3737.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 155 765 1222 1251
Size: 155 610 457 29
Fragments arranged by size:
610 457 155 29
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1161 1251
Size: 1161 90
ApaI G_GGCC'C
Cuts at: 0 19 1030 1251
Size: 19 1011 221
Fragments arranged by size:
1011 221 19
ApaLI G'TGCA_C
Cuts at: 0 142 533 1251
Size: 142 391 718
Fragments arranged by size:
718 391 142
BanI G'GyrC_C
Cuts at: 0 821 1079 1251
Size: 821 258 172
Fragments arranged by size:
821 258 172
BanII G_rGCy'C
Cuts at: 0 19 1030 1226 1251
Size: 19 1011 196 25
Fragments arranged by size:
1011 196 25 19
BbvCI CC'TCA_GC
Cuts at: 0 114 1251
Size: 114 1137
BciVI GTATCCnnnnn_n'
Cuts at: 0 540 1251
Size: 540 711
BclI T'GATC_A
Cuts at: 0 1037 1251
Size: 1037 214
BglI GCCn_nnn'nGGC
Cuts at: 0 448 1251
Size: 448 803
BglII A'GATC_T
Cuts at: 0 871 1251
Size: 871 380
Bme1580I G_kGCm'C
Cuts at: 0 19 146 265 459 537 720 824 1030
Size: 19 127 119 194 78 183 104 206
Cuts at: 1030 1104 1251
Size: 74 147
Fragments arranged by size:
206 194 183 147 127 119 104 78
74 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 22 423 1203 1251
Size: 22 401 780 48
Fragments arranged by size:
780 401 48 22
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 811 957 1251
Size: 696 115 146 294
Fragments arranged by size:
696 294 146 115
Bpu10I CC'TnA_GC
Cuts at: 0 114 964 1251
Size: 114 850 287
Fragments arranged by size:
850 287 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 342 1251
Size: 342 909
BsaWI w'CCGG_w
Cuts at: 0 866 1251
Size: 866 385
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 376 406 1251
Size: 376 30 845
Fragments arranged by size:
845 376 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 808 997 1000 1251
Size: 400 408 189 3 251
Fragments arranged by size:
408 400 251 189 3
BseYI C'CCAG_C
Cuts at: 0 18 30 366 406 606 727 1199 1251
Size: 18 12 336 40 200 121 472 52
Fragments arranged by size:
472 336 200 121 52 40 18 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 944 1251
Size: 925 19 307
Fragments arranged by size:
925 307 19
BsiEI CG_ry'CG
Cuts at: 0 654 1251
Size: 654 597
BsiHKAI G_wGCw'C
Cuts at: 0 146 537 953 1251
Size: 146 391 416 298
Fragments arranged by size:
416 391 298 146
Bsp1286I G_dGCh'C
Cuts at: 0 19 146 265 459 537 720 824 953
Size: 19 127 119 194 78 183 104 129
Cuts at: 953 1030 1104 1226 1251
Size: 77 74 122 25
Fragments arranged by size:
194 183 129 127 122 119 104 78
77 74 25 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 155 453 765 1222 1251
Size: 155 298 312 457 29
Fragments arranged by size:
457 312 298 155 29
BsrDI GCAATG_nn'
Cuts at: 0 624 1251
Size: 624 627
BsrFI r'CCGG_y
Cuts at: 0 649 940 1251
Size: 649 291 311
Fragments arranged by size:
649 311 291
BstAPI GCAn_nnn'nTGC
Cuts at: 0 322 1251
Size: 322 929
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 1251
Size: 12 1239
BstYI r'GATC_y
Cuts at: 0 332 871 1110 1251
Size: 332 539 239 141
Fragments arranged by size:
539 332 239 141
Bsu36I CC'TnA_GG
Cuts at: 0 485 1251
Size: 485 766
BtgI C'CryG_G
Cuts at: 0 180 579 1083 1089 1251
Size: 180 399 504 6 162
Fragments arranged by size:
504 399 180 162 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 769 1072 1251
Size: 769 303 179
Fragments arranged by size:
769 303 179
BtsI GCAGTG_nn'
Cuts at: 0 377 1251
Size: 377 874
DraIII CAC_nnn'GTG
Cuts at: 0 124 1251
Size: 124 1127
EaeI y'GGCC_r
Cuts at: 0 57 183 651 1086 1144 1251
Size: 57 126 468 435 58 107
Fragments arranged by size:
468 435 126 107 58 57
EagI C'GGCC_G
Cuts at: 0 651 1251
Size: 651 600
EarI CTCTTCn'nnn_
Cuts at: 0 727 1251
Size: 727 524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 844 1251
Size: 844 407
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 811 957 1161 1251
Size: 696 115 146 204 90
Fragments arranged by size:
696 204 146 115 90
EcoNI CCTnn'n_nnAGG
Cuts at: 0 70 793 889 1251
Size: 70 723 96 362
Fragments arranged by size:
723 362 96 70
EcoO109I rG'GnC_Cy
Cuts at: 0 75 960 1027 1251
Size: 75 885 67 224
Fragments arranged by size:
885 224 75 67
HaeII r_GCGC'y
Cuts at: 0 1098 1251
Size: 1098 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1156 1188 1251
Size: 1156 32 63
Fragments arranged by size:
1156 63 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 804 1251
Size: 665 139 447
Fragments arranged by size:
665 447 139
MscI TGG'CCA
Cuts at: 0 59 185 1088 1146 1251
Size: 59 126 903 58 105
Fragments arranged by size:
903 126 105 59 58
MslI CAynn'nnrTG
Cuts at: 0 916 1001 1251
Size: 916 85 250
Fragments arranged by size:
916 250 85
MspA1I CmG'CkG
Cuts at: 0 656 1077 1181 1251
Size: 656 421 104 70
Fragments arranged by size:
656 421 104 70
NaeI GCC'GGC
Cuts at: 0 942 1251
Size: 942 309
NcoI C'CATG_G
Cuts at: 0 579 1083 1251
Size: 579 504 168
Fragments arranged by size:
579 504 168
NgoMIV G'CCGG_C
Cuts at: 0 940 1251
Size: 940 311
PasI CC'CwG_GG
Cuts at: 0 257 1251
Size: 257 994
PflMI CCAn_nnn'nTGG
Cuts at: 0 613 1251
Size: 613 638
PfoI T'CCnGG_A
Cuts at: 0 1113 1251
Size: 1113 138
PpuMI rG'GwC_Cy
Cuts at: 0 75 960 1251
Size: 75 885 291
Fragments arranged by size:
885 291 75
PspOMI G'GGCC_C
Cuts at: 0 15 1026 1251
Size: 15 1011 225
Fragments arranged by size:
1011 225 15
PstI C_TGCA'G
Cuts at: 0 1069 1251
Size: 1069 182
PvuII CAG'CTG
Cuts at: 0 1181 1251
Size: 1181 70
SexAI A'CCwGG_T
Cuts at: 0 1010 1251
Size: 1010 241
SfcI C'TryA_G
Cuts at: 0 1065 1251
Size: 1065 186
StuI AGG'CCT
Cuts at: 0 505 772 799 1230 1251
Size: 505 267 27 431 21
Fragments arranged by size:
505 431 267 27 21
StyI C'CwwG_G
Cuts at: 0 579 1022 1083 1158 1251
Size: 579 443 61 75 93
Fragments arranged by size:
579 443 93 75 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 759 1251
Size: 759 492
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 376 408 1251
Size: 376 32 843
Fragments arranged by size:
843 376 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1056 1081 1251
Size: 1056 25 170
Fragments arranged by size:
1056 170 25
XmnI GAAnn'nnTTC
Cuts at: 0 1130 1174 1251
Size: 1130 44 77
Fragments arranged by size:
1130 77 44
Enzymes that do cut:
AarI AcuI ApaI ApaLI BanI BanII BbvCI BciVI
BclI BglI BglII Bme1580I BmrI BpmI Bpu10I BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SexAI SfcI StuI StyI TaqII TstI
XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BcgI
BfrBI BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI
BsaHI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI DraI
DrdI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI NarI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI
XmaI ZraI