Restriction map for
pF1KB3734
(Linear) (Six-base) MAPSORT of: KIBB3734.seq Check: 6732 from: 1 to: 1383
KIBB3734.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 163 1294 1383
Size: 122 41 1131 89
Fragments arranged by size:
1131 122 89 41
AvaI C'yCGr_G
Cuts at: 0 1229 1383
Size: 1229 154
BamHI G'GATC_C
Cuts at: 0 96 1383
Size: 96 1287
BanII G_rGCy'C
Cuts at: 0 407 1253 1383
Size: 407 846 130
Fragments arranged by size:
846 407 130
BglII A'GATC_T
Cuts at: 0 341 1383
Size: 341 1042
BmgBI CAC'GTC
Cuts at: 0 796 1383
Size: 796 587
BmrI ACTGGGnnnn_n'
Cuts at: 0 207 469 1383
Size: 207 262 914
Fragments arranged by size:
914 262 207
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 86 1383
Size: 63 23 1297
Fragments arranged by size:
1297 63 23
Bpu10I CC'TnA_GC
Cuts at: 0 125 1383
Size: 125 1258
BsaBI GATnn'nnATC
Cuts at: 0 1088 1383
Size: 1088 295
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1309 1339 1383
Size: 1309 30 44
Fragments arranged by size:
1309 44 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 1383
Size: 232 1151
BseYI C'CCAG_C
Cuts at: 0 987 1383
Size: 987 396
BsiHKAI G_wGCw'C
Cuts at: 0 407 1289 1383
Size: 407 882 94
Fragments arranged by size:
882 407 94
BsmI GAATG_Cn'
Cuts at: 0 1135 1383
Size: 1135 248
Bsp1286I G_dGCh'C
Cuts at: 0 407 1253 1289 1383
Size: 407 846 36 94
Fragments arranged by size:
846 407 94 36
BspHI T'CATG_A
Cuts at: 0 810 1383
Size: 810 573
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1055 1383
Size: 1055 328
BsrGI T'GTAC_A
Cuts at: 0 2 1262 1383
Size: 2 1260 121
Fragments arranged by size:
1260 121 2
BstYI r'GATC_y
Cuts at: 0 96 341 920 1383
Size: 96 245 579 463
Fragments arranged by size:
579 463 245 96
BtgI C'CryG_G
Cuts at: 0 395 1383
Size: 395 988
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 44 1383
Size: 44 1339
BtsI GCAGTG_nn'
Cuts at: 0 691 1223 1383
Size: 691 532 160
Fragments arranged by size:
691 532 160
DraIII CAC_nnn'GTG
Cuts at: 0 1212 1383
Size: 1212 171
EaeI y'GGCC_r
Cuts at: 0 1187 1383
Size: 1187 196
EarI CTCTTCn'nnn_
Cuts at: 0 113 182 565 628 674 1383
Size: 113 69 383 63 46 709
Fragments arranged by size:
709 383 113 69 63 46
EcoICRI GAG'CTC
Cuts at: 0 405 1383
Size: 405 978
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 86 122 163 1294 1383
Size: 63 23 36 41 1131 89
Fragments arranged by size:
1131 89 63 41 36 23
EcoO109I rG'GnC_Cy
Cuts at: 0 254 1383
Size: 254 1129
EcoRV GAT'ATC
Cuts at: 0 1086 1383
Size: 1086 297
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 912 944 1383
Size: 912 32 439
Fragments arranged by size:
912 439 32
HindIII A'AGCT_T
Cuts at: 0 650 851 1383
Size: 650 201 532
Fragments arranged by size:
650 532 201
MslI CAynn'nnrTG
Cuts at: 0 353 1058 1383
Size: 353 705 325
Fragments arranged by size:
705 353 325
MspA1I CmG'CkG
Cuts at: 0 912 1383
Size: 912 471
PpuMI rG'GwC_Cy
Cuts at: 0 254 1383
Size: 254 1129
PvuII CAG'CTG
Cuts at: 0 912 1383
Size: 912 471
SacI G_AGCT'C
Cuts at: 0 407 1383
Size: 407 976
SapI GCTCTTCn'nnn_
Cuts at: 0 113 674 1383
Size: 113 561 709
Fragments arranged by size:
709 561 113
SexAI A'CCwGG_T
Cuts at: 0 1069 1383
Size: 1069 314
SfcI C'TryA_G
Cuts at: 0 1218 1383
Size: 1218 165
SspI AAT'ATT
Cuts at: 0 519 674 1383
Size: 519 155 709
Fragments arranged by size:
709 519 155
StyI C'CwwG_G
Cuts at: 0 1307 1383
Size: 1307 76
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 790 1383
Size: 790 593
TatI w'GTAC_w
Cuts at: 0 2 440 1095 1262 1383
Size: 2 438 655 167 121
Fragments arranged by size:
655 438 167 121 2
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1309 1341 1383
Size: 1309 32 42
Fragments arranged by size:
1309 42 32
XbaI T'CTAG_A
Cuts at: 0 828 1383
Size: 828 555
Enzymes that do cut:
AcuI AvaI BamHI BanII BglII BmgBI BmrI BpmI
Bpu10I BsaBI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrGI BstYI BtgI BtgZI BtsI DraIII
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV Hin4I HindIII
MslI MspA1I PpuMI PvuII SacI SapI SexAI SfcI
SspI StyI TaqII TatI TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BanI BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
Bme1580I BmtI BplI BpuEI BsaI BsaAI BsaHI BsaWI
BsgI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EagI EciI EcoNI EcoRI
FalI FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XcmI XhoI XmaI XmnI ZraI