Restriction map for
pF1KB3732
(Linear) (Six-base) MAPSORT of: KIBB3732.seq Check: 1659 from: 1 to: 1383
KIBB3732.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 789 1102 1124 1383
Size: 119 670 313 22 259
Fragments arranged by size:
670 313 259 119 22
AflIII A'CryG_T
Cuts at: 0 367 1383
Size: 367 1016
AlwNI CAG_nnn'CTG
Cuts at: 0 713 1383
Size: 713 670
ApoI r'AATT_y
Cuts at: 0 730 1383
Size: 730 653
AvaI C'yCGr_G
Cuts at: 0 1149 1383
Size: 1149 234
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 345 378 1383
Size: 345 33 1005
Fragments arranged by size:
1005 345 33
BanII G_rGCy'C
Cuts at: 0 317 641 662 1383
Size: 317 324 21 721
Fragments arranged by size:
721 324 317 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 749 902 1073 1130 1383
Size: 749 153 171 57 253
Fragments arranged by size:
749 253 171 153 57
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 461 495 1383
Size: 461 34 888
Fragments arranged by size:
888 461 34
BfrBI ATG'CAT
Cuts at: 0 506 1383
Size: 506 877
BglI GCCn_nnn'nGGC
Cuts at: 0 688 1383
Size: 688 695
BglII A'GATC_T
Cuts at: 0 1278 1383
Size: 1278 105
Bme1580I G_kGCm'C
Cuts at: 0 249 629 1383
Size: 249 380 754
Fragments arranged by size:
754 380 249
BmrI ACTGGGnnnn_n'
Cuts at: 0 287 1383
Size: 287 1096
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 1383
Size: 225 1158
BsaAI yAC'GTr
Cuts at: 0 368 1239 1383
Size: 368 871 144
Fragments arranged by size:
871 368 144
BsaHI Gr'CG_yC
Cuts at: 0 1052 1383
Size: 1052 331
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 922 952 1383
Size: 922 30 431
Fragments arranged by size:
922 431 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 592 901 1383
Size: 280 312 309 482
Fragments arranged by size:
482 312 309 280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1303 1383
Size: 1303 80
BsiHKAI G_wGCw'C
Cuts at: 0 317 662 1383
Size: 317 345 721
Fragments arranged by size:
721 345 317
BsmI GAATG_Cn'
Cuts at: 0 1339 1383
Size: 1339 44
Bsp1286I G_dGCh'C
Cuts at: 0 249 317 629 641 662 1383
Size: 249 68 312 12 21 721
Fragments arranged by size:
721 312 249 68 21 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 849 1383
Size: 849 534
BstYI r'GATC_y
Cuts at: 0 1278 1383
Size: 1278 105
BtsI GCAGTG_nn'
Cuts at: 0 1010 1383
Size: 1010 373
DraIII CAC_nnn'GTG
Cuts at: 0 1239 1383
Size: 1239 144
DrdI GACnn_nn'nnGTC
Cuts at: 0 235 1383
Size: 235 1148
EaeI y'GGCC_r
Cuts at: 0 557 1252 1383
Size: 557 695 131
Fragments arranged by size:
695 557 131
EarI CTCTTCn'nnn_
Cuts at: 0 112 599 1354 1383
Size: 112 487 755 29
Fragments arranged by size:
755 487 112 29
EcoICRI GAG'CTC
Cuts at: 0 315 660 1383
Size: 315 345 723
Fragments arranged by size:
723 345 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 789 1102 1124 1383
Size: 119 670 313 22 259
Fragments arranged by size:
670 313 259 119 22
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1117 1267 1383
Size: 1117 150 116
Fragments arranged by size:
1117 150 116
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 405 810 995 1383
Size: 405 405 185 388
Fragments arranged by size:
405 405 388 185
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 922 954 1383
Size: 922 32 429
Fragments arranged by size:
922 429 32
HincII GTy'rAC
Cuts at: 0 792 1383
Size: 792 591
MscI TGG'CCA
Cuts at: 0 559 1254 1383
Size: 559 695 129
Fragments arranged by size:
695 559 129
MslI CAynn'nnrTG
Cuts at: 0 218 1327 1383
Size: 218 1109 56
Fragments arranged by size:
1109 218 56
MspA1I CmG'CkG
Cuts at: 0 359 1020 1383
Size: 359 661 363
Fragments arranged by size:
661 363 359
NsiI A_TGCA'T
Cuts at: 0 508 1383
Size: 508 875
NspI r_CATG'y
Cuts at: 0 1074 1383
Size: 1074 309
PflMI CCAn_nnn'nTGG
Cuts at: 0 781 1383
Size: 781 602
PmlI CAC'GTG
Cuts at: 0 368 1239 1383
Size: 368 871 144
Fragments arranged by size:
871 368 144
PstI C_TGCA'G
Cuts at: 0 614 1383
Size: 614 769
PvuII CAG'CTG
Cuts at: 0 1020 1383
Size: 1020 363
SacI G_AGCT'C
Cuts at: 0 317 662 1383
Size: 317 345 721
Fragments arranged by size:
721 345 317
SapI GCTCTTCn'nnn_
Cuts at: 0 112 599 1383
Size: 112 487 784
Fragments arranged by size:
784 487 112
SfcI C'TryA_G
Cuts at: 0 610 1383
Size: 610 773
SmaI CCC'GGG
Cuts at: 0 1151 1383
Size: 1151 232
SmlI C'TyrA_G
Cuts at: 0 240 1383
Size: 240 1143
SspI AAT'ATT
Cuts at: 0 200 1383
Size: 200 1183
StyI C'CwwG_G
Cuts at: 0 702 1383
Size: 702 681
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 80 1383
Size: 80 1303
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 158 190 1383
Size: 158 32 1193
Fragments arranged by size:
1193 158 32
Tth111I GACn'n_nGTC
Cuts at: 0 463 1383
Size: 463 920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1024 1062 1383
Size: 1024 38 321
Fragments arranged by size:
1024 321 38
XmaI C'CCGG_G
Cuts at: 0 1149 1383
Size: 1149 234
Enzymes that do cut:
AcuI AflIII AlwNI ApoI AvaI BaeI BanII BbsI
BcgI BfrBI BglI BglII Bme1580I BmrI BpuEI BsaAI
BsaHI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BstXI
BstYI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI
EcoNI FalI Hin4I HincII MscI MslI MspA1I NsiI
NspI PflMI PmlI PstI PvuII SacI SapI SfcI
SmaI SmlI SspI StyI TaqII TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BamHI BanI BbeI BbvCI BciVI BclI
BlpI BmgBI BmtI BplI BpmI Bpu10I BsaI BsaBI
BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
EagI EciI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI StuI SwaI TatI XbaI
XhoI XmnI ZraI