Restriction map for
pF1KB3717
(Linear) (Six-base) MAPSORT of: KIBB3717.seq Check: 19 from: 1 to: 1434
KIBB3717.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AccI GT'mk_AC
Cuts at: 0 98 181 936 1269 1434
Size: 98 83 755 333 165
Fragments arranged by size:
755 333 165 98 83
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1294 1434
Size: 1294 140
AhdI GACnn_n'nnGTC
Cuts at: 0 1265 1434
Size: 1265 169
AlwNI CAG_nnn'CTG
Cuts at: 0 867 983 1434
Size: 867 116 451
Fragments arranged by size:
867 451 116
ApaLI G'TGCA_C
Cuts at: 0 445 995 1434
Size: 445 550 439
Fragments arranged by size:
550 445 439
AseI AT'TA_AT
Cuts at: 0 743 1106 1434
Size: 743 363 328
Fragments arranged by size:
743 363 328
AvaI C'yCGr_G
Cuts at: 0 77 242 1434
Size: 77 165 1192
Fragments arranged by size:
1192 165 77
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 90 123 1434
Size: 90 33 1311
Fragments arranged by size:
1311 90 33
BamHI G'GATC_C
Cuts at: 0 1384 1434
Size: 1384 50
BanII G_rGCy'C
Cuts at: 0 961 1434
Size: 961 473
BbsI GAAGACnn'nnnn_
Cuts at: 0 75 662 795 1088 1434
Size: 75 587 133 293 346
Fragments arranged by size:
587 346 293 133 75
BclI T'GATC_A
Cuts at: 0 1298 1434
Size: 1298 136
Bme1580I G_kGCm'C
Cuts at: 0 449 999 1434
Size: 449 550 435
Fragments arranged by size:
550 449 435
BmrI ACTGGGnnnn_n'
Cuts at: 0 1213 1434
Size: 1213 221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1434
Size: 671 763
Bpu10I CC'TnA_GC
Cuts at: 0 960 1434
Size: 960 474
BsaI GGTCTCn'nnnn_
Cuts at: 0 229 356 371 1434
Size: 229 127 15 1063
Fragments arranged by size:
1063 229 127 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 623 653 1434
Size: 623 30 781
Fragments arranged by size:
781 623 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 1434
Size: 232 1202
BseYI C'CCAG_C
Cuts at: 0 1332 1434
Size: 1332 102
BsiHKAI G_wGCw'C
Cuts at: 0 449 999 1419 1434
Size: 449 550 420 15
Fragments arranged by size:
550 449 420 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1252 1434
Size: 1252 182
Bsp1286I G_dGCh'C
Cuts at: 0 449 961 999 1419 1434
Size: 449 512 38 420 15
Fragments arranged by size:
512 449 420 38 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 812 1434
Size: 812 622
BsrFI r'CCGG_y
Cuts at: 0 925 1434
Size: 925 509
BsrGI T'GTAC_A
Cuts at: 0 858 1434
Size: 858 576
BstBI TT'CG_AA
Cuts at: 0 1428 1434
Size: 1428 6
BstEII G'GTnAC_C
Cuts at: 0 51 1434
Size: 51 1383
BstXI CCAn_nnnn'nTGG
Cuts at: 0 373 1434
Size: 373 1061
BstYI r'GATC_y
Cuts at: 0 221 1384 1434
Size: 221 1163 50
Fragments arranged by size:
1163 221 50
BstZ17I GTA'TAC
Cuts at: 0 182 937 1434
Size: 182 755 497
Fragments arranged by size:
755 497 182
Bsu36I CC'TnA_GG
Cuts at: 0 454 941 1434
Size: 454 487 493
Fragments arranged by size:
493 487 454
BtgI C'CryG_G
Cuts at: 0 1208 1388 1434
Size: 1208 180 46
Fragments arranged by size:
1208 180 46
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 665 700 1434
Size: 665 35 734
Fragments arranged by size:
734 665 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1294 1434
Size: 671 623 140
Fragments arranged by size:
671 623 140
EcoO109I rG'GnC_Cy
Cuts at: 0 792 1434
Size: 792 642
EcoRV GAT'ATC
Cuts at: 0 582 1434
Size: 582 852
HaeII r_GCGC'y
Cuts at: 0 1333 1434
Size: 1333 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1251 1283 1434
Size: 1251 32 151
Fragments arranged by size:
1251 151 32
HincII GTy'rAC
Cuts at: 0 519 1270 1434
Size: 519 751 164
Fragments arranged by size:
751 519 164
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 1434
Size: 354 1080
MslI CAynn'nnrTG
Cuts at: 0 1006 1324 1434
Size: 1006 318 110
Fragments arranged by size:
1006 318 110
MspA1I CmG'CkG
Cuts at: 0 1405 1434
Size: 1405 29
NaeI GCC'GGC
Cuts at: 0 927 1434
Size: 927 507
NcoI C'CATG_G
Cuts at: 0 1208 1388 1434
Size: 1208 180 46
Fragments arranged by size:
1208 180 46
NgoMIV G'CCGG_C
Cuts at: 0 925 1434
Size: 925 509
NspI r_CATG'y
Cuts at: 0 1068 1373 1434
Size: 1068 305 61
Fragments arranged by size:
1068 305 61
PpuMI rG'GwC_Cy
Cuts at: 0 792 1434
Size: 792 642
PshAI GACnn'nnGTC
Cuts at: 0 58 1275 1434
Size: 58 1217 159
Fragments arranged by size:
1217 159 58
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 172 204 1434
Size: 172 32 1230
Fragments arranged by size:
1230 172 32
PstI C_TGCA'G
Cuts at: 0 897 1434
Size: 897 537
SalI G'TCGA_C
Cuts at: 0 1268 1434
Size: 1268 166
SfcI C'TryA_G
Cuts at: 0 95 270 893 1434
Size: 95 175 623 541
Fragments arranged by size:
623 541 175 95
SmaI CCC'GGG
Cuts at: 0 79 1434
Size: 79 1355
SphI G_CATG'C
Cuts at: 0 1068 1373 1434
Size: 1068 305 61
Fragments arranged by size:
1068 305 61
SspI AAT'ATT
Cuts at: 0 297 411 902 1434
Size: 297 114 491 532
Fragments arranged by size:
532 491 297 114
StuI AGG'CCT
Cuts at: 0 346 1434
Size: 346 1088
StyI C'CwwG_G
Cuts at: 0 1208 1388 1434
Size: 1208 180 46
Fragments arranged by size:
1208 180 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 785 1014 1085 1324 1434
Size: 785 229 71 239 110
Fragments arranged by size:
785 239 229 110 71
TatI w'GTAC_w
Cuts at: 0 858 1434
Size: 858 576
Tth111I GACn'n_nGTC
Cuts at: 0 33 1434
Size: 33 1401
XmaI C'CCGG_G
Cuts at: 0 77 1434
Size: 77 1357
Enzymes that do cut:
AccI AcuI AhdI AlwNI ApaLI AseI AvaI BaeI
BamHI BanII BbsI BclI Bme1580I BmrI BpmI Bpu10I
BsaI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI
BsrFI BsrGI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI CspCI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII
MmeI MslI MspA1I NaeI NcoI NgoMIV NspI PpuMI
PshAI PsrI PstI SalI SfcI SmaI SphI SspI
StuI StyI TaqII TatI Tth111I XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApoI AscI AsiSI AvrII
BanI BbeI BbvCI BcgI BciVI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI
BsaWI BsgI BsiEI BsiWI BsmI BspEI BspHI BsrBI
BsrDI BssHII BssSI BstAPI BtgZI BtsI ClaI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI
EcoRI FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MscI NarI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PsiI PspOMI PspXI PvuI PvuII RsrII
SacI SacII SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SwaI
TstI XbaI XcmI XhoI XmnI ZraI