Restriction map for
pF1KB3705
(Linear) (Six-base) MAPSORT of: KIBB3705.seq Check: 7381 from: 1 to: 3210
KIBB3705.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AccI GT'mk_AC
Cuts at: 0 2366 3210
Size: 2366 844
Acc65I G'GTAC_C
Cuts at: 0 135 3210
Size: 135 3075
AclI AA'CG_TT
Cuts at: 0 690 3210
Size: 690 2520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 1756 1768 3210
Size: 449 1307 12 1442
Fragments arranged by size:
1442 1307 449 12
AflII C'TTAA_G
Cuts at: 0 3070 3210
Size: 3070 140
AflIII A'CryG_T
Cuts at: 0 623 3210
Size: 623 2587
AhdI GACnn_n'nnGTC
Cuts at: 0 2274 3210
Size: 2274 936
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2069 3210
Size: 2069 1141
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 533 565 3210
Size: 533 32 2645
Fragments arranged by size:
2645 533 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1779 3210
Size: 1779 1431
ApaLI G'TGCA_C
Cuts at: 0 2044 3210
Size: 2044 1166
ApoI r'AATT_y
Cuts at: 0 406 439 460 918 1156 1480 2698 2829
Size: 406 33 21 458 238 324 1218 131
Cuts at: 2829 3130 3210
Size: 301 80
Fragments arranged by size:
1218 458 406 324 301 238 131 80
33 21
AseI AT'TA_AT
Cuts at: 0 1166 1259 3210
Size: 1166 93 1951
Fragments arranged by size:
1951 1166 93
AvaI C'yCGr_G
Cuts at: 0 140 1808 3210
Size: 140 1668 1402
Fragments arranged by size:
1668 1402 140
BamHI G'GATC_C
Cuts at: 0 423 1632 3210
Size: 423 1209 1578
Fragments arranged by size:
1578 1209 423
BanI G'GyrC_C
Cuts at: 0 135 3210
Size: 135 3075
BanII G_rGCy'C
Cuts at: 0 831 1088 3210
Size: 831 257 2122
Fragments arranged by size:
2122 831 257
BbsI GAAGACnn'nnnn_
Cuts at: 0 297 1379 3210
Size: 297 1082 1831
Fragments arranged by size:
1831 1082 297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1673 1707 3210
Size: 1673 34 1503
Fragments arranged by size:
1673 1503 34
BclI T'GATC_A
Cuts at: 0 777 1131 3210
Size: 777 354 2079
Fragments arranged by size:
2079 777 354
BfrBI ATG'CAT
Cuts at: 0 2226 2965 3210
Size: 2226 739 245
Fragments arranged by size:
2226 739 245
BglI GCCn_nnn'nGGC
Cuts at: 0 901 2358 3210
Size: 901 1457 852
Fragments arranged by size:
1457 901 852
BglII A'GATC_T
Cuts at: 0 3205 3210
Size: 3205 5
BlpI GC'TnA_GC
Cuts at: 0 1766 3210
Size: 1766 1444
Bme1580I G_kGCm'C
Cuts at: 0 1965 2048 2114 3210
Size: 1965 83 66 1096
Fragments arranged by size:
1965 1096 83 66
BmgBI CAC'GTC
Cuts at: 0 624 3210
Size: 624 2586
BmrI ACTGGGnnnn_n'
Cuts at: 0 122 3210
Size: 122 3088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1382 3210
Size: 1382 1828
Bpu10I CC'TnA_GC
Cuts at: 0 3145 3210
Size: 3145 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1774 3210
Size: 1774 1436
BsaBI GATnn'nnATC
Cuts at: 0 121 2966 3210
Size: 121 2845 244
Fragments arranged by size:
2845 244 121
BsaWI w'CCGG_w
Cuts at: 0 806 3210
Size: 806 2404
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 3210
Size: 283 2927
BsiHKAI G_wGCw'C
Cuts at: 0 1088 1992 2048 2566 3210
Size: 1088 904 56 518 644
Fragments arranged by size:
1088 904 644 518 56
BsmI GAATG_Cn'
Cuts at: 0 859 2190 3210
Size: 859 1331 1020
Fragments arranged by size:
1331 1020 859
Bsp1286I G_dGCh'C
Cuts at: 0 831 1088 1965 1992 2048 2114 2566 3210
Size: 831 257 877 27 56 66 452 644
Fragments arranged by size:
877 831 644 452 257 66 56 27
BspEI T'CCGG_A
Cuts at: 0 806 3210
Size: 806 2404
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2124 2279 3210
Size: 2124 155 931
Fragments arranged by size:
2124 931 155
BsrDI GCAATG_nn'
Cuts at: 0 2610 3210
Size: 2610 600
BsrFI r'CCGG_y
Cuts at: 0 2939 3210
Size: 2939 271
BstBI TT'CG_AA
Cuts at: 0 444 3210
Size: 444 2766
BstXI CCAn_nnnn'nTGG
Cuts at: 0 93 3210
Size: 93 3117
BstYI r'GATC_y
Cuts at: 0 423 1632 1665 2496 3205 3210
Size: 423 1209 33 831 709 5
Fragments arranged by size:
1209 831 709 423 33 5
BstZ17I GTA'TAC
Cuts at: 0 2367 3210
Size: 2367 843
BtsI GCAGTG_nn'
Cuts at: 0 1566 1714 3210
Size: 1566 148 1496
Fragments arranged by size:
1566 1496 148
DraI TTT'AAA
Cuts at: 0 1344 2165 2237 2412 3210
Size: 1344 821 72 175 798
Fragments arranged by size:
1344 821 798 175 72
EaeI y'GGCC_r
Cuts at: 0 2790 2937 3210
Size: 2790 147 273
Fragments arranged by size:
2790 273 147
EarI CTCTTCn'nnn_
Cuts at: 0 941 2803 3001 3088 3210
Size: 941 1862 198 87 122
Fragments arranged by size:
1862 941 198 122 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2935 3210
Size: 2935 275
EcoICRI GAG'CTC
Cuts at: 0 1086 3210
Size: 1086 2124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 1382 1756 1768 3210
Size: 449 933 374 12 1442
Fragments arranged by size:
1442 933 449 374 12
EcoO109I rG'GnC_Cy
Cuts at: 0 1815 3210
Size: 1815 1395
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 589 2656 3210
Size: 589 2067 554
Fragments arranged by size:
2067 589 554
FspI TGC'GCA
Cuts at: 0 457 3210
Size: 457 2753
HaeII r_GCGC'y
Cuts at: 0 1780 3145 3210
Size: 1780 1365 65
Fragments arranged by size:
1780 1365 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 211 243 1624 1656 2260 2292 3190 3210
Size: 211 32 1381 32 604 32 898 20
Fragments arranged by size:
1381 898 604 211 32 32 32 20
HincII GTy'rAC
Cuts at: 0 2444 3023 3210
Size: 2444 579 187
Fragments arranged by size:
2444 579 187
KpnI G_GTAC'C
Cuts at: 0 139 3210
Size: 139 3071
MfeI C'AATT_G
Cuts at: 0 1351 2576 3210
Size: 1351 1225 634
Fragments arranged by size:
1351 1225 634
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 2379 3210
Size: 110 2269 831
Fragments arranged by size:
2269 831 110
MscI TGG'CCA
Cuts at: 0 2792 3210
Size: 2792 418
MslI CAynn'nnrTG
Cuts at: 0 91 2119 3210
Size: 91 2028 1091
Fragments arranged by size:
2028 1091 91
MspA1I CmG'CkG
Cuts at: 0 752 1382 1546 2592 3210
Size: 752 630 164 1046 618
Fragments arranged by size:
1046 752 630 618 164
NsiI A_TGCA'T
Cuts at: 0 2228 2967 3210
Size: 2228 739 243
Fragments arranged by size:
2228 739 243
NspI r_CATG'y
Cuts at: 0 245 1477 3210
Size: 245 1232 1733
Fragments arranged by size:
1733 1232 245
PasI CC'CwG_GG
Cuts at: 0 352 3210
Size: 352 2858
PstI C_TGCA'G
Cuts at: 0 2276 2619 3030 3210
Size: 2276 343 411 180
Fragments arranged by size:
2276 411 343 180
PvuII CAG'CTG
Cuts at: 0 1382 1546 2592 3210
Size: 1382 164 1046 618
Fragments arranged by size:
1382 1046 618 164
SacI G_AGCT'C
Cuts at: 0 1088 3210
Size: 1088 2122
SapI GCTCTTCn'nnn_
Cuts at: 0 2803 3210
Size: 2803 407
SfcI C'TryA_G
Cuts at: 0 1310 1868 2272 2615 3026 3201 3210
Size: 1310 558 404 343 411 175 9
Fragments arranged by size:
1310 558 411 404 343 175 9
SmaI CCC'GGG
Cuts at: 0 1810 3210
Size: 1810 1400
SmlI C'TyrA_G
Cuts at: 0 1753 3070 3210
Size: 1753 1317 140
Fragments arranged by size:
1753 1317 140
SspI AAT'ATT
Cuts at: 0 1170 1373 3210
Size: 1170 203 1837
Fragments arranged by size:
1837 1170 203
StyI C'CwwG_G
Cuts at: 0 2742 3210
Size: 2742 468
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2699 3210
Size: 2699 511
TatI w'GTAC_w
Cuts at: 0 2064 3210
Size: 2064 1146
XbaI T'CTAG_A
Cuts at: 0 1969 3210
Size: 1969 1241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2809 3210
Size: 2809 401
XmaI C'CCGG_G
Cuts at: 0 1808 3210
Size: 1808 1402
XmnI GAAnn'nnTTC
Cuts at: 0 966 2995 3210
Size: 966 2029 215
Fragments arranged by size:
2029 966 215
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AhdI AlfI
AloI AlwNI ApaLI ApoI AseI AvaI BamHI BanI
BanII BbsI BcgI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaBI BsaWI
BseRI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BsrFI
BstBI BstXI BstYI BstZ17I BtsI DraI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I
HincII KpnI MfeI MmeI MscI MslI MspA1I NsiI
NspI PasI PstI PvuII SacI SapI SfcI SmaI
SmlI SspI StyI TaqII TatI XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AleI ApaI AscI AsiSI
AvrII BaeI BbeI BbvCI BciVI BmtI BplI BsaI
BsaAI BsaHI BsaXI BseYI BsgI BsiEI BsiWI BsmBI
BspHI BsrBI BsrGI BssHII BssSI BstAPI BstEII Bsu36I
BtgI BtgZI ClaI CspCI DraIII DrdI EagI EcoNI
EcoRI EcoRV FseI FspAI HindIII HpaI KasI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I XhoI ZraI