Restriction map for
pF1KB3690
(Linear) (Six-base) MAPSORT of: KIBB3690.seq Check: 9387 from: 1 to: 1272
KIBB3690.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AccI GT'mk_AC
Cuts at: 0 935 1272
Size: 935 337
AclI AA'CG_TT
Cuts at: 0 72 1272
Size: 72 1200
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 739 1112 1272
Size: 514 225 373 160
Fragments arranged by size:
514 373 225 160
AhdI GACnn_n'nnGTC
Cuts at: 0 624 1272
Size: 624 648
AlwNI CAG_nnn'CTG
Cuts at: 0 84 850 1212 1272
Size: 84 766 362 60
Fragments arranged by size:
766 362 84 60
ApaI G_GGCC'C
Cuts at: 0 108 1272
Size: 108 1164
ApaLI G'TGCA_C
Cuts at: 0 528 1272
Size: 528 744
ApoI r'AATT_y
Cuts at: 0 298 808 1007 1272
Size: 298 510 199 265
Fragments arranged by size:
510 298 265 199
AseI AT'TA_AT
Cuts at: 0 328 1272
Size: 328 944
AvaI C'yCGr_G
Cuts at: 0 675 1272
Size: 675 597
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 778 811 1272
Size: 778 33 461
Fragments arranged by size:
778 461 33
BanI G'GyrC_C
Cuts at: 0 576 1272
Size: 576 696
BanII G_rGCy'C
Cuts at: 0 108 1272
Size: 108 1164
BbeI G_GCGC'C
Cuts at: 0 580 1272
Size: 580 692
BbsI GAAGACnn'nnnn_
Cuts at: 0 1160 1272
Size: 1160 112
BciVI GTATCCnnnnn_n'
Cuts at: 0 806 1272
Size: 806 466
BclI T'GATC_A
Cuts at: 0 693 1272
Size: 693 579
BglII A'GATC_T
Cuts at: 0 885 1272
Size: 885 387
Bme1580I G_kGCm'C
Cuts at: 0 108 532 1272
Size: 108 424 740
Fragments arranged by size:
740 424 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 1014 1272
Size: 1014 258
BsaAI yAC'GTr
Cuts at: 0 555 1272
Size: 555 717
BsaHI Gr'CG_yC
Cuts at: 0 577 1272
Size: 577 695
BseYI C'CCAG_C
Cuts at: 0 720 1272
Size: 720 552
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 1272
Size: 471 801
BsiHKAI G_wGCw'C
Cuts at: 0 532 1272
Size: 532 740
BsmI GAATG_Cn'
Cuts at: 0 426 1272
Size: 426 846
Bsp1286I G_dGCh'C
Cuts at: 0 108 532 1272
Size: 108 424 740
Fragments arranged by size:
740 424 108
BspHI T'CATG_A
Cuts at: 0 372 539 743 1272
Size: 372 167 204 529
Fragments arranged by size:
529 372 204 167
BstYI r'GATC_y
Cuts at: 0 497 885 926 1272
Size: 497 388 41 346
Fragments arranged by size:
497 388 346 41
ClaI AT'CG_AT
Cuts at: 0 1174 1272
Size: 1174 98
EarI CTCTTCn'nnn_
Cuts at: 0 675 1272
Size: 675 597
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 739 1112 1272
Size: 514 225 373 160
Fragments arranged by size:
514 373 225 160
EcoO109I rG'GnC_Cy
Cuts at: 0 104 1252 1272
Size: 104 1148 20
Fragments arranged by size:
1148 104 20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 194 1272
Size: 194 1078
HaeII r_GCGC'y
Cuts at: 0 580 1272
Size: 580 692
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 195 227 610 642 877 909 1174 1206
Size: 195 32 383 32 235 32 265 32
Cuts at: 1206 1272
Size: 66
Fragments arranged by size:
383 265 235 195 66 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 576 1272
Size: 576 696
MfeI C'AATT_G
Cuts at: 0 92 1043 1272
Size: 92 951 229
Fragments arranged by size:
951 229 92
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1004 1272
Size: 1004 268
MslI CAynn'nnrTG
Cuts at: 0 472 1272
Size: 472 800
MspA1I CmG'CkG
Cuts at: 0 84 433 720 1272
Size: 84 349 287 552
Fragments arranged by size:
552 349 287 84
NarI GG'CG_CC
Cuts at: 0 577 1272
Size: 577 695
NspI r_CATG'y
Cuts at: 0 1042 1272
Size: 1042 230
PfoI T'CCnGG_A
Cuts at: 0 798 1272
Size: 798 474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 209 241 1272
Size: 209 32 1031
Fragments arranged by size:
1031 209 32
PpuMI rG'GwC_Cy
Cuts at: 0 1252 1272
Size: 1252 20
PshAI GACnn'nnGTC
Cuts at: 0 1232 1272
Size: 1232 40
PspOMI G'GGCC_C
Cuts at: 0 104 1272
Size: 104 1168
PvuII CAG'CTG
Cuts at: 0 84 433 720 1272
Size: 84 349 287 552
Fragments arranged by size:
552 349 287 84
SanDI GG'GwC_CC
Cuts at: 0 1252 1272
Size: 1252 20
SfcI C'TryA_G
Cuts at: 0 932 1272
Size: 932 340
SfoI GGC'GCC
Cuts at: 0 578 1272
Size: 578 694
SmaI CCC'GGG
Cuts at: 0 677 1272
Size: 677 595
StyI C'CwwG_G
Cuts at: 0 108 1272
Size: 108 1164
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 968 1272
Size: 968 304
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 105 137 1272
Size: 105 32 1135
Fragments arranged by size:
1135 105 32
XbaI T'CTAG_A
Cuts at: 0 291 1272
Size: 291 981
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 406 1272
Size: 406 866
XmaI C'CCGG_G
Cuts at: 0 675 1272
Size: 675 597
XmnI GAAnn'nnTTC
Cuts at: 0 299 821 1272
Size: 299 522 451
Fragments arranged by size:
522 451 299
Enzymes that do cut:
AccI AclI AcuI AhdI AlwNI ApaI ApaLI ApoI
AseI AvaI BaeI BanI BanII BbeI BbsI BciVI
BclI BglII Bme1580I BmrI BsaAI BsaHI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BstYI ClaI EarI Eco57MI
EcoO109I FalI HaeII Hin4I KasI MfeI MmeI MslI
MspA1I NarI NspI PfoI PpiI PpuMI PshAI PspOMI
PvuII SanDI SfcI SfoI SmaI StyI TaqII TstI
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AleI
AlfI AloI AscI AsiSI AvrII BamHI BbvCI BcgI
BfrBI BglI BlpI BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MluI MscI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XhoI ZraI