Restriction map for
pF1KB3683
(Linear) (Six-base) MAPSORT of: KIBB3683.seq Check: 863 from: 1 to: 3522
KIBB3683.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1451 3522
Size: 1451 2071
AatII G_ACGT'C
Cuts at: 0 1776 3522
Size: 1776 1746
AccI GT'mk_AC
Cuts at: 0 1429 1866 2402 2811 3522
Size: 1429 437 536 409 711
Fragments arranged by size:
1429 711 536 437 409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 277 403 1817 2400 3522
Size: 227 50 126 1414 583 1122
Fragments arranged by size:
1414 1122 583 227 126 50
AflIII A'CryG_T
Cuts at: 0 2497 3522
Size: 2497 1025
AhdI GACnn_n'nnGTC
Cuts at: 0 2933 3522
Size: 2933 589
AleI CACnn'nnGTG
Cuts at: 0 3182 3522
Size: 3182 340
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1088 1120 3522
Size: 1088 32 2402
Fragments arranged by size:
2402 1088 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3416 3522
Size: 3416 106
ApoI r'AATT_y
Cuts at: 0 269 452 678 954 1227 1261 1455 1572
Size: 269 183 226 276 273 34 194 117
Cuts at: 1572 1627 1653 1664 2749 2800 3522
Size: 55 26 11 1085 51 722
Fragments arranged by size:
1085 722 276 273 269 226 194 183
117 55 51 34 26 11
AseI AT'TA_AT
Cuts at: 0 560 3522
Size: 560 2962
BamHI G'GATC_C
Cuts at: 0 537 3522
Size: 537 2985
BanI G'GyrC_C
Cuts at: 0 736 3522
Size: 736 2786
BbsI GAAGACnn'nnnn_
Cuts at: 0 185 2378 3522
Size: 185 2193 1144
Fragments arranged by size:
2193 1144 185
BciVI GTATCCnnnnn_n'
Cuts at: 0 1090 3522
Size: 1090 2432
BclI T'GATC_A
Cuts at: 0 1362 1808 2142 2469 3522
Size: 1362 446 334 327 1053
Fragments arranged by size:
1362 1053 446 334 327
BglI GCCn_nnn'nGGC
Cuts at: 0 1585 3522
Size: 1585 1937
BglII A'GATC_T
Cuts at: 0 3232 3522
Size: 3232 290
BlpI GC'TnA_GC
Cuts at: 0 212 3522
Size: 212 3310
BmrI ACTGGGnnnn_n'
Cuts at: 0 1365 3294 3522
Size: 1365 1929 228
Fragments arranged by size:
1929 1365 228
BmtI G_CTAG'C
Cuts at: 0 1486 3522
Size: 1486 2036
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1412 2018 2549 3522
Size: 1412 606 531 973
Fragments arranged by size:
1412 973 606 531
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 3522
Size: 694 2828
BsaI GGTCTCn'nnnn_
Cuts at: 0 1066 1531 3522
Size: 1066 465 1991
Fragments arranged by size:
1991 1066 465
BsaAI yAC'GTr
Cuts at: 0 356 2500 3522
Size: 356 2144 1022
Fragments arranged by size:
2144 1022 356
BsaBI GATnn'nnATC
Cuts at: 0 85 506 1783 1837 3338 3522
Size: 85 421 1277 54 1501 184
Fragments arranged by size:
1501 1277 421 184 85 54
BsaHI Gr'CG_yC
Cuts at: 0 1773 3522
Size: 1773 1749
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2576 2606 3522
Size: 2576 30 916
Fragments arranged by size:
2576 916 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 176 740 986 3522
Size: 176 564 246 2536
Fragments arranged by size:
2536 564 246 176
BseYI C'CCAG_C
Cuts at: 0 1186 1715 3522
Size: 1186 529 1807
Fragments arranged by size:
1807 1186 529
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3043 3522
Size: 3043 479
BsiHKAI G_wGCw'C
Cuts at: 0 312 2647 3522
Size: 312 2335 875
Fragments arranged by size:
2335 875 312
BsmI GAATG_Cn'
Cuts at: 0 532 1201 2953 3412 3455 3522
Size: 532 669 1752 459 43 67
Fragments arranged by size:
1752 669 532 459 67 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1466 1764 2692 3522
Size: 1466 298 928 830
Fragments arranged by size:
1466 928 830 298
Bsp1286I G_dGCh'C
Cuts at: 0 312 2647 3522
Size: 312 2335 875
Fragments arranged by size:
2335 875 312
BspHI T'CATG_A
Cuts at: 0 2384 3522
Size: 2384 1138
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1451 3522
Size: 1451 2071
BsrDI GCAATG_nn'
Cuts at: 0 2033 2342 3522
Size: 2033 309 1180
Fragments arranged by size:
2033 1180 309
BsrGI T'GTAC_A
Cuts at: 0 3083 3522
Size: 3083 439
BssSI C'ACGA_G
Cuts at: 0 3182 3236 3522
Size: 3182 54 286
Fragments arranged by size:
3182 286 54
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1681 3416 3522
Size: 1681 1735 106
Fragments arranged by size:
1735 1681 106
BstYI r'GATC_y
Cuts at: 0 537 879 1179 1247 1603 1734 1838 1962
Size: 537 342 300 68 356 131 104 124
Cuts at: 1962 3203 3232 3333 3522
Size: 1241 29 101 189
Fragments arranged by size:
1241 537 356 342 300 189 131 124
104 101 68 29
Bsu36I CC'TnA_GG
Cuts at: 0 1759 3522
Size: 1759 1763
BtgI C'CryG_G
Cuts at: 0 2406 3221 3288 3522
Size: 2406 815 67 234
Fragments arranged by size:
2406 815 234 67
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 233 268 3345 3380 3522
Size: 233 35 3077 35 142
Fragments arranged by size:
3077 233 142 35 35
DraI TTT'AAA
Cuts at: 0 1454 3522
Size: 1454 2068
DraIII CAC_nnn'GTG
Cuts at: 0 2086 3522
Size: 2086 1436
DrdI GACnn_nn'nnGTC
Cuts at: 0 1425 2283 3522
Size: 1425 858 1239
Fragments arranged by size:
1425 1239 858
EaeI y'GGCC_r
Cuts at: 0 1970 2339 3522
Size: 1970 369 1183
Fragments arranged by size:
1970 1183 369
EarI CTCTTCn'nnn_
Cuts at: 0 739 1131 1903 3522
Size: 739 392 772 1619
Fragments arranged by size:
1619 772 739 392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 277 403 1412 1817 2018 2400 2549
Size: 227 50 126 1009 405 201 382 149
Cuts at: 2549 3522
Size: 973
Fragments arranged by size:
1009 973 405 382 227 201 149 126
50
EcoO109I rG'GnC_Cy
Cuts at: 0 3078 3193 3392 3522
Size: 3078 115 199 130
Fragments arranged by size:
3078 199 130 115
EcoRI G'AATT_C
Cuts at: 0 678 3522
Size: 678 2844
EcoRV GAT'ATC
Cuts at: 0 2892 3522
Size: 2892 630
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 433 3522
Size: 433 3089
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 414 446 649 681 1164 1196 2192 2224
Size: 414 32 203 32 483 32 996 32
Cuts at: 2224 2919 2951 3021 3053 3165 3197 3217 3249
Size: 695 32 70 32 112 32 20 32
Cuts at: 3249 3522
Size: 273
Fragments arranged by size:
996 695 483 414 273 203 112 70
32 32 32 32 32 32 32 32
20
HincII GTy'rAC
Cuts at: 0 473 2812 3018 3522
Size: 473 2339 206 504
Fragments arranged by size:
2339 504 473 206
HindIII A'AGCT_T
Cuts at: 0 920 3522
Size: 920 2602
HpaI GTT'AAC
Cuts at: 0 3018 3522
Size: 3018 504
MfeI C'AATT_G
Cuts at: 0 1924 2333 3522
Size: 1924 409 1189
Fragments arranged by size:
1924 1189 409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1581 1701 3522
Size: 863 718 120 1821
Fragments arranged by size:
1821 863 718 120
MscI TGG'CCA
Cuts at: 0 1972 2341 3522
Size: 1972 369 1181
Fragments arranged by size:
1972 1181 369
MslI CAynn'nnrTG
Cuts at: 0 221 811 1052 1789 3182 3401 3405 3522
Size: 221 590 241 737 1393 219 4 117
Fragments arranged by size:
1393 737 590 241 221 219 117 4
MspA1I CmG'CkG
Cuts at: 0 129 1194 2398 2408 3522
Size: 129 1065 1204 10 1114
Fragments arranged by size:
1204 1114 1065 129 10
NcoI C'CATG_G
Cuts at: 0 3221 3288 3522
Size: 3221 67 234
Fragments arranged by size:
3221 234 67
NdeI CA'TA_TG
Cuts at: 0 3158 3402 3522
Size: 3158 244 120
Fragments arranged by size:
3158 244 120
NheI G'CTAG_C
Cuts at: 0 1482 3522
Size: 1482 2040
NspI r_CATG'y
Cuts at: 0 77 220 717 2510 2527 3464 3522
Size: 77 143 497 1793 17 937 58
Fragments arranged by size:
1793 937 497 143 77 58 17
PflMI CCAn_nnn'nTGG
Cuts at: 0 2791 3522
Size: 2791 731
PmlI CAC'GTG
Cuts at: 0 2500 3522
Size: 2500 1022
PpuMI rG'GwC_Cy
Cuts at: 0 3078 3193 3392 3522
Size: 3078 115 199 130
Fragments arranged by size:
3078 199 130 115
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3076 3108 3522
Size: 3076 32 414
Fragments arranged by size:
3076 414 32
PstI C_TGCA'G
Cuts at: 0 1677 3522
Size: 1677 1845
PvuII CAG'CTG
Cuts at: 0 129 2398 3522
Size: 129 2269 1124
Fragments arranged by size:
2269 1124 129
SacII CC_GC'GG
Cuts at: 0 2409 3522
Size: 2409 1113
SalI G'TCGA_C
Cuts at: 0 2810 3522
Size: 2810 712
ScaI AGT'ACT
Cuts at: 0 699 3522
Size: 699 2823
SfcI C'TryA_G
Cuts at: 0 191 199 1556 1673 1950 2399 2673 3522
Size: 191 8 1357 117 277 449 274 849
Fragments arranged by size:
1357 849 449 277 274 191 117 8
SmlI C'TyrA_G
Cuts at: 0 673 3522
Size: 673 2849
SpeI A'CTAG_T
Cuts at: 0 2692 3522
Size: 2692 830
SphI G_CATG'C
Cuts at: 0 77 220 717 2527 3464 3522
Size: 77 143 497 1810 937 58
Fragments arranged by size:
1810 937 497 143 77 58
SspI AAT'ATT
Cuts at: 0 3122 3522
Size: 3122 400
StyI C'CwwG_G
Cuts at: 0 2109 3221 3288 3522
Size: 2109 1112 67 234
Fragments arranged by size:
2109 1112 234 67
TatI w'GTAC_w
Cuts at: 0 697 3083 3522
Size: 697 2386 439
Fragments arranged by size:
2386 697 439
Tth111I GACn'n_nGTC
Cuts at: 0 2816 3522
Size: 2816 706
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1980 3522
Size: 1980 1542
XmnI GAAnn'nnTTC
Cuts at: 0 416 575 2951 3522
Size: 416 159 2376 571
Fragments arranged by size:
2376 571 416 159
ZraI GAC'GTC
Cuts at: 0 1774 3522
Size: 1774 1748
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AloI
AlwNI ApoI AseI BamHI BanI BbsI BciVI BclI
BglI BglII BlpI BmrI BmtI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI
BstAPI BstYI Bsu36I BtgI CspCI DraI DraIII DrdI
EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I
HincII HindIII HpaI MfeI MmeI MscI MslI MspA1I
NcoI NdeI NheI NspI PflMI PmlI PpuMI PsrI
PstI PvuII SacII SalI ScaI SfcI SmlI SpeI
SphI SspI StyI TatI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AlfI ApaI ApaLI
AscI AsiSI AvaI AvrII BaeI BanII BbeI BbvCI
BcgI BfrBI Bme1580I BmgBI BplI Bpu10I BsaWI BsiEI
BsiWI BspEI BsrBI BsrFI BssHII BstBI BstEII BstXI
BstZ17I BtgZI BtsI ClaI EagI EciI EcoICRI EcoNI
FseI FspI FspAI HaeII KasI KpnI MluI NaeI
NarI NgoMIV NotI NruI NsiI PacI PasI PciI
PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PvuI
RsrII SacI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SrfI StuI SwaI TaqII
TstI XbaI XhoI XmaI