Restriction map for
pF1KB3682
(Linear) (Six-base) MAPSORT of: KIBB3682.seq Check: 3431 from: 1 to: 2256
KIBB3682.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1232 1718 2256
Size: 1232 486 538
Fragments arranged by size:
1232 538 486
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 259 352 906 1307 2017 2140 2156
Size: 10 249 93 554 401 710 123 16
Cuts at: 2156 2256
Size: 100
Fragments arranged by size:
710 554 401 249 123 100 93 16
10
AflIII A'CryG_T
Cuts at: 0 1565 2256
Size: 1565 691
AhdI GACnn_n'nnGTC
Cuts at: 0 186 2205 2256
Size: 186 2019 51
Fragments arranged by size:
2019 186 51
AleI CACnn'nnGTG
Cuts at: 0 1168 1852 2256
Size: 1168 684 404
Fragments arranged by size:
1168 684 404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 550 2256
Size: 550 1706
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1871 1903 2256
Size: 1871 32 353
Fragments arranged by size:
1871 353 32
AlwNI CAG_nnn'CTG
Cuts at: 0 105 2256
Size: 105 2151
ApaLI G'TGCA_C
Cuts at: 0 1481 2256
Size: 1481 775
ApoI r'AATT_y
Cuts at: 0 43 347 1213 1656 2230 2256
Size: 43 304 866 443 574 26
Fragments arranged by size:
866 574 443 304 43 26
AseI AT'TA_AT
Cuts at: 0 701 2256
Size: 701 1555
AvaI C'yCGr_G
Cuts at: 0 1348 1398 1826 2256
Size: 1348 50 428 430
Fragments arranged by size:
1348 430 428 50
BanI G'GyrC_C
Cuts at: 0 1220 1252 2256
Size: 1220 32 1004
Fragments arranged by size:
1220 1004 32
BanII G_rGCy'C
Cuts at: 0 952 1494 1825 2224 2256
Size: 952 542 331 399 32
Fragments arranged by size:
952 542 399 331 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 299 1018 2256
Size: 299 719 1238
Fragments arranged by size:
1238 719 299
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 805 839 1344 1378 2256
Size: 805 34 505 34 878
Fragments arranged by size:
878 805 505 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2018 2256
Size: 2018 238
BclI T'GATC_A
Cuts at: 0 201 314 576 2256
Size: 201 113 262 1680
Fragments arranged by size:
1680 262 201 113
BlpI GC'TnA_GC
Cuts at: 0 1493 2256
Size: 1493 763
Bme1580I G_kGCm'C
Cuts at: 0 1159 1485 1773 2256
Size: 1159 326 288 483
Fragments arranged by size:
1159 483 326 288
BmrI ACTGGGnnnn_n'
Cuts at: 0 141 2256
Size: 141 2115
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1314 1374 2256
Size: 1314 60 882
Fragments arranged by size:
1314 882 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 2034 2256
Size: 70 1964 222
Fragments arranged by size:
1964 222 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 2256
Size: 586 1670
BsaI GGTCTCn'nnnn_
Cuts at: 0 547 1549 1615 2256
Size: 547 1002 66 641
Fragments arranged by size:
1002 641 547 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1697 1727 2256
Size: 1697 30 529
Fragments arranged by size:
1697 529 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1198 2206 2256
Size: 1198 1008 50
Fragments arranged by size:
1198 1008 50
BsiHKAI G_wGCw'C
Cuts at: 0 140 403 952 1485 1825 2256
Size: 140 263 549 533 340 431
Fragments arranged by size:
549 533 431 340 263 140
BsmI GAATG_Cn'
Cuts at: 0 696 2256
Size: 696 1560
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1345 2256
Size: 1345 911
Bsp1286I G_dGCh'C
Cuts at: 0 140 403 952 1159 1485 1494 1773 1825
Size: 140 263 549 207 326 9 279 52
Cuts at: 1825 2224 2256
Size: 399 32
Fragments arranged by size:
549 399 326 279 263 207 140 52
32 9
BspHI T'CATG_A
Cuts at: 0 177 2256
Size: 177 2079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 45 1232 1718 2180 2256
Size: 45 1187 486 462 76
Fragments arranged by size:
1187 486 462 76 45
BsrBI CCG'CTC
Cuts at: 0 1403 1860 2256
Size: 1403 457 396
Fragments arranged by size:
1403 457 396
BssSI C'ACGA_G
Cuts at: 0 550 2256
Size: 550 1706
BstXI CCAn_nnnn'nTGG
Cuts at: 0 564 2035 2256
Size: 564 1471 221
Fragments arranged by size:
1471 564 221
Bsu36I CC'TnA_GG
Cuts at: 0 2189 2256
Size: 2189 67
BtgI C'CryG_G
Cuts at: 0 86 1618 2256
Size: 86 1532 638
Fragments arranged by size:
1532 638 86
DraIII CAC_nnn'GTG
Cuts at: 0 1480 2256
Size: 1480 776
DrdI GACnn_nn'nnGTC
Cuts at: 0 439 2256
Size: 439 1817
EarI CTCTTCn'nnn_
Cuts at: 0 1283 2256
Size: 1283 973
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1456 2256
Size: 1456 800
EcoICRI GAG'CTC
Cuts at: 0 950 1823 2256
Size: 950 873 433
Fragments arranged by size:
950 873 433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 70 259 352 906 1307 2017 2034
Size: 10 60 189 93 554 401 710 17
Cuts at: 2034 2140 2156 2256
Size: 106 16 100
Fragments arranged by size:
710 554 401 189 106 100 93 60
17 16 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 1777 2256
Size: 52 1725 479
Fragments arranged by size:
1725 479 52
EcoO109I rG'GnC_Cy
Cuts at: 0 1270 1796 2256
Size: 1270 526 460
Fragments arranged by size:
1270 526 460
EcoRI G'AATT_C
Cuts at: 0 2230 2256
Size: 2230 26
HaeII r_GCGC'y
Cuts at: 0 743 2256
Size: 743 1513
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 357 389 1344 1376 2191 2223
Size: 172 32 153 32 955 32 815 32
Cuts at: 2223 2256
Size: 33
Fragments arranged by size:
955 815 172 153 33 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1579 2256
Size: 1579 677
MfeI C'AATT_G
Cuts at: 0 1228 1318 2256
Size: 1228 90 938
Fragments arranged by size:
1228 938 90
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 1406 2256
Size: 824 582 850
Fragments arranged by size:
850 824 582
MslI CAynn'nnrTG
Cuts at: 0 406 1168 1435 1848 1852 2256
Size: 406 762 267 413 4 404
Fragments arranged by size:
762 413 406 404 267 4
MspA1I CmG'CkG
Cuts at: 0 195 326 594 780 1002 1560 2256
Size: 195 131 268 186 222 558 696
Fragments arranged by size:
696 558 268 222 195 186 131
NcoI C'CATG_G
Cuts at: 0 86 2256
Size: 86 2170
NspI r_CATG'y
Cuts at: 0 981 1434 1569 2256
Size: 981 453 135 687
Fragments arranged by size:
981 687 453 135
PciI A'CATG_T
Cuts at: 0 1565 2256
Size: 1565 691
PflMI CCAn_nnn'nTGG
Cuts at: 0 1421 2256
Size: 1421 835
PfoI T'CCnGG_A
Cuts at: 0 47 2256
Size: 47 2209
PpuMI rG'GwC_Cy
Cuts at: 0 1270 1796 2256
Size: 1270 526 460
Fragments arranged by size:
1270 526 460
PshAI GACnn'nnGTC
Cuts at: 0 1018 2256
Size: 1018 1238
PsiI TTA'TAA
Cuts at: 0 1073 2256
Size: 1073 1183
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 581 613 2256
Size: 581 32 1643
Fragments arranged by size:
1643 581 32
PstI C_TGCA'G
Cuts at: 0 785 818 2256
Size: 785 33 1438
Fragments arranged by size:
1438 785 33
PvuII CAG'CTG
Cuts at: 0 195 326 594 780 1560 2256
Size: 195 131 268 186 780 696
Fragments arranged by size:
780 696 268 195 186 131
SacI G_AGCT'C
Cuts at: 0 952 1825 2256
Size: 952 873 431
Fragments arranged by size:
952 873 431
SapI GCTCTTCn'nnn_
Cuts at: 0 1283 2256
Size: 1283 973
SfcI C'TryA_G
Cuts at: 0 241 781 797 814 2225 2256
Size: 241 540 16 17 1411 31
Fragments arranged by size:
1411 540 241 31 17 16
SmaI CCC'GGG
Cuts at: 0 1828 2256
Size: 1828 428
SmlI C'TyrA_G
Cuts at: 0 601 1348 2256
Size: 601 747 908
Fragments arranged by size:
908 747 601
SpeI A'CTAG_T
Cuts at: 0 711 2256
Size: 711 1545
SphI G_CATG'C
Cuts at: 0 981 2256
Size: 981 1275
SspI AAT'ATT
Cuts at: 0 393 1980 2016 2256
Size: 393 1587 36 240
Fragments arranged by size:
1587 393 240 36
StyI C'CwwG_G
Cuts at: 0 86 2256
Size: 86 2170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1721 1842 2256
Size: 1721 121 414
Fragments arranged by size:
1721 414 121
XbaI T'CTAG_A
Cuts at: 0 2149 2256
Size: 2149 107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 768 1200 1484 2256
Size: 768 432 284 772
Fragments arranged by size:
772 768 432 284
XhoI C'TCGA_G
Cuts at: 0 1348 2256
Size: 1348 908
XmaI C'CCGG_G
Cuts at: 0 1826 2256
Size: 1826 430
XmnI GAAnn'nnTTC
Cuts at: 0 232 644 790 1292 2256
Size: 232 412 146 502 964
Fragments arranged by size:
964 502 412 232 146
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlfI AloI AlwNI
ApaLI ApoI AseI AvaI BanI BanII BbsI BcgI
BciVI BclI BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaXI BseRI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BssSI BstXI Bsu36I BtgI DraIII DrdI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HindIII MfeI MmeI MslI MspA1I NcoI NspI
PciI PflMI PfoI PpuMI PshAI PsiI PsrI PstI
PvuII SacI SapI SfcI SmaI SmlI SpeI SphI
SspI StyI TaqII XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI ApaI
AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BfrBI
BglI BglII BmgBI BmtI Bpu10I BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiWI BspEI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I BtgZI
BtsI ClaI CspCI DraI EaeI EagI EcoRV FalI
FseI FspI FspAI HincII HpaI KasI KpnI MluI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PspOMI PspXI
PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SrfI StuI SwaI
TatI TstI Tth111I ZraI