Restriction map for
pF1KB3671
(Linear) (Six-base) MAPSORT of: KIBB3671.seq Check: 2820 from: 1 to: 1128
KIBB3671.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AclI AA'CG_TT
Cuts at: 0 307 1128
Size: 307 821
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 367 1128
Size: 245 122 761
Fragments arranged by size:
761 245 122
AflIII A'CryG_T
Cuts at: 0 80 1128
Size: 80 1048
AleI CACnn'nnGTG
Cuts at: 0 226 1128
Size: 226 902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1038 1128
Size: 1038 90
AlwNI CAG_nnn'CTG
Cuts at: 0 171 252 648 1046 1128
Size: 171 81 396 398 82
Fragments arranged by size:
398 396 171 82 81
ApaLI G'TGCA_C
Cuts at: 0 943 1128
Size: 943 185
ApoI r'AATT_y
Cuts at: 0 811 1128
Size: 811 317
AvrII C'CTAG_G
Cuts at: 0 129 1128
Size: 129 999
BanI G'GyrC_C
Cuts at: 0 976 1128
Size: 976 152
BanII G_rGCy'C
Cuts at: 0 716 1128
Size: 716 412
BbsI GAAGACnn'nnnn_
Cuts at: 0 197 316 1128
Size: 197 119 812
Fragments arranged by size:
812 197 119
BlpI GC'TnA_GC
Cuts at: 0 341 676 715 1128
Size: 341 335 39 413
Fragments arranged by size:
413 341 335 39
Bme1580I G_kGCm'C
Cuts at: 0 947 1128
Size: 947 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 1128
Size: 670 458
BsaI GGTCTCn'nnnn_
Cuts at: 0 411 914 1128
Size: 411 503 214
Fragments arranged by size:
503 411 214
BsaAI yAC'GTr
Cuts at: 0 624 1128
Size: 624 504
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 592 622 1128
Size: 592 30 506
Fragments arranged by size:
592 506 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 96 583 1128
Size: 96 487 545
Fragments arranged by size:
545 487 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1128
Size: 604 524
BsiHKAI G_wGCw'C
Cuts at: 0 716 947 1128
Size: 716 231 181
Fragments arranged by size:
716 231 181
BsmI GAATG_Cn'
Cuts at: 0 1097 1128
Size: 1097 31
Bsp1286I G_dGCh'C
Cuts at: 0 716 947 1128
Size: 716 231 181
Fragments arranged by size:
716 231 181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 260 1103 1128
Size: 260 843 25
Fragments arranged by size:
843 260 25
BsrDI GCAATG_nn'
Cuts at: 0 911 1128
Size: 911 217
BssHII G'CGCG_C
Cuts at: 0 636 638 1128
Size: 636 2 490
Fragments arranged by size:
636 490 2
BtgI C'CryG_G
Cuts at: 0 119 1128
Size: 119 1009
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1106 1128
Size: 1106 22
DraI TTT'AAA
Cuts at: 0 47 179 555 816 1128
Size: 47 132 376 261 312
Fragments arranged by size:
376 312 261 132 47
DraIII CAC_nnn'GTG
Cuts at: 0 81 1128
Size: 81 1047
EaeI y'GGCC_r
Cuts at: 0 116 263 1128
Size: 116 147 865
Fragments arranged by size:
865 147 116
EarI CTCTTCn'nnn_
Cuts at: 0 371 428 775 1128
Size: 371 57 347 353
Fragments arranged by size:
371 353 347 57
EcoICRI GAG'CTC
Cuts at: 0 714 1128
Size: 714 414
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 367 670 1128
Size: 245 122 303 458
Fragments arranged by size:
458 303 245 122
EcoNI CCTnn'n_nnAGG
Cuts at: 0 663 1128
Size: 663 465
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 44 755 820 912 1128
Size: 44 711 65 92 216
Fragments arranged by size:
711 216 92 65 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 42 74 579 611 826 858 1128
Size: 42 32 505 32 215 32 270
Fragments arranged by size:
505 270 215 42 32 32 32
MfeI C'AATT_G
Cuts at: 0 455 1128
Size: 455 673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 903 1128
Size: 903 225
MscI TGG'CCA
Cuts at: 0 118 1128
Size: 118 1010
MslI CAynn'nnrTG
Cuts at: 0 226 1128
Size: 226 902
MspA1I CmG'CkG
Cuts at: 0 645 913 988 1128
Size: 645 268 75 140
Fragments arranged by size:
645 268 140 75
NcoI C'CATG_G
Cuts at: 0 119 1128
Size: 119 1009
PfoI T'CCnGG_A
Cuts at: 0 382 1128
Size: 382 746
PmlI CAC'GTG
Cuts at: 0 624 1128
Size: 624 504
PshAI GACnn'nnGTC
Cuts at: 0 926 1128
Size: 926 202
PstI C_TGCA'G
Cuts at: 0 375 1128
Size: 375 753
PvuII CAG'CTG
Cuts at: 0 645 1128
Size: 645 483
SacI G_AGCT'C
Cuts at: 0 716 1128
Size: 716 412
ScaI AGT'ACT
Cuts at: 0 786 1128
Size: 786 342
SfcI C'TryA_G
Cuts at: 0 371 1128
Size: 371 757
StyI C'CwwG_G
Cuts at: 0 119 129 1128
Size: 119 10 999
Fragments arranged by size:
999 119 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 433 1128
Size: 433 695
TatI w'GTAC_w
Cuts at: 0 784 1128
Size: 784 344
XmnI GAAnn'nnTTC
Cuts at: 0 194 1128
Size: 194 934
Enzymes that do cut:
AclI AcuI AflIII AleI AlfI AlwNI ApaLI ApoI
AvrII BanI BanII BbsI BlpI Bme1580I BpmI BsaI
BsaAI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BssHII BtgI BtgZI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI FalI Hin4I MfeI MmeI MscI
MslI MspA1I NcoI PfoI PmlI PshAI PstI PvuII
SacI ScaI SfcI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvaI BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaBI BsaHI
BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtsI ClaI CspCI DrdI EagI EciI
EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI