Restriction map for
pF1KB3657
(Linear) (Six-base) MAPSORT of: KIBB3657.seq Check: 2838 from: 1 to: 1080
KIBB3657.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 282 1080
Size: 282 798
AccI GT'mk_AC
Cuts at: 0 845 1080
Size: 845 235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 679 1080
Size: 511 168 401
Fragments arranged by size:
511 401 168
AflIII A'CryG_T
Cuts at: 0 144 1080
Size: 144 936
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 651 683 1080
Size: 651 32 397
Fragments arranged by size:
651 397 32
AlwNI CAG_nnn'CTG
Cuts at: 0 36 78 1080
Size: 36 42 1002
Fragments arranged by size:
1002 42 36
ApoI r'AATT_y
Cuts at: 0 292 398 1080
Size: 292 106 682
Fragments arranged by size:
682 292 106
AvaI C'yCGr_G
Cuts at: 0 61 1080
Size: 61 1019
BanI G'GyrC_C
Cuts at: 0 57 97 826 1080
Size: 57 40 729 254
Fragments arranged by size:
729 254 57 40
BanII G_rGCy'C
Cuts at: 0 94 570 1080
Size: 94 476 510
Fragments arranged by size:
510 476 94
BbeI G_GCGC'C
Cuts at: 0 101 1080
Size: 101 979
BbsI GAAGACnn'nnnn_
Cuts at: 0 485 1080
Size: 485 595
BclI T'GATC_A
Cuts at: 0 969 1080
Size: 969 111
BfrBI ATG'CAT
Cuts at: 0 594 632 1080
Size: 594 38 448
Fragments arranged by size:
594 448 38
BglII A'GATC_T
Cuts at: 0 333 1080
Size: 333 747
Bme1580I G_kGCm'C
Cuts at: 0 62 1080
Size: 62 1018
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 150 307 874 1080
Size: 68 82 157 567 206
Fragments arranged by size:
567 206 157 82 68
BsaI GGTCTCn'nnnn_
Cuts at: 0 287 1080
Size: 287 793
BsaAI yAC'GTr
Cuts at: 0 145 546 1080
Size: 145 401 534
Fragments arranged by size:
534 401 145
BsaBI GATnn'nnATC
Cuts at: 0 305 1080
Size: 305 775
BsaHI Gr'CG_yC
Cuts at: 0 98 1080
Size: 98 982
Bsp1286I G_dGCh'C
Cuts at: 0 62 94 570 1080
Size: 62 32 476 510
Fragments arranged by size:
510 476 62 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 282 1080
Size: 282 798
BsrFI r'CCGG_y
Cuts at: 0 26 1080
Size: 26 1054
BstAPI GCAn_nnn'nTGC
Cuts at: 0 705 1080
Size: 705 375
BstEII G'GTnAC_C
Cuts at: 0 312 1080
Size: 312 768
BstXI CCAn_nnnn'nTGG
Cuts at: 0 837 1080
Size: 837 243
BstYI r'GATC_y
Cuts at: 0 333 1080
Size: 333 747
BtsI GCAGTG_nn'
Cuts at: 0 158 1080
Size: 158 922
DraIII CAC_nnn'GTG
Cuts at: 0 557 1080
Size: 557 523
EaeI y'GGCC_r
Cuts at: 0 548 638 956 1080
Size: 548 90 318 124
Fragments arranged by size:
548 318 124 90
EarI CTCTTCn'nnn_
Cuts at: 0 1061 1080
Size: 1061 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 68 150 307 511 679 874 1080
Size: 68 82 157 204 168 195 206
Fragments arranged by size:
206 204 195 168 157 82 68
EcoO109I rG'GnC_Cy
Cuts at: 0 447 990 1080
Size: 447 543 90
Fragments arranged by size:
543 447 90
EcoRI G'AATT_C
Cuts at: 0 292 1080
Size: 292 788
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 822 1080
Size: 822 258
HaeII r_GCGC'y
Cuts at: 0 101 1080
Size: 101 979
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 604 636 693 725 1060 1080
Size: 604 32 57 32 335 20
Fragments arranged by size:
604 335 57 32 32 20
HincII GTy'rAC
Cuts at: 0 650 846 1080
Size: 650 196 234
Fragments arranged by size:
650 234 196
HindIII A'AGCT_T
Cuts at: 0 361 1080
Size: 361 719
KasI G'GCGC_C
Cuts at: 0 97 1080
Size: 97 983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1080
Size: 239 841
MscI TGG'CCA
Cuts at: 0 550 1080
Size: 550 530
MslI CAynn'nnrTG
Cuts at: 0 242 524 1080
Size: 242 282 556
Fragments arranged by size:
556 282 242
MspA1I CmG'CkG
Cuts at: 0 430 895 1080
Size: 430 465 185
Fragments arranged by size:
465 430 185
NarI GG'CG_CC
Cuts at: 0 98 1080
Size: 98 982
NdeI CA'TA_TG
Cuts at: 0 230 1080
Size: 230 850
NsiI A_TGCA'T
Cuts at: 0 596 634 1080
Size: 596 38 446
Fragments arranged by size:
596 446 38
NspI r_CATG'y
Cuts at: 0 916 1080
Size: 916 164
PflMI CCAn_nnn'nTGG
Cuts at: 0 324 557 1080
Size: 324 233 523
Fragments arranged by size:
523 324 233
PpuMI rG'GwC_Cy
Cuts at: 0 447 990 1080
Size: 447 543 90
Fragments arranged by size:
543 447 90
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 203 235 1080
Size: 203 32 845
Fragments arranged by size:
845 203 32
PvuII CAG'CTG
Cuts at: 0 430 1080
Size: 430 650
SalI G'TCGA_C
Cuts at: 0 844 1080
Size: 844 236
SfcI C'TryA_G
Cuts at: 0 204 1080
Size: 204 876
SfoI GGC'GCC
Cuts at: 0 99 1080
Size: 99 981
Enzymes that do cut:
AarI AccI AcuI AflIII AloI AlwNI ApoI AvaI
BanI BanII BbeI BbsI BclI BfrBI BglII Bme1580I
BpmI BsaI BsaAI BsaBI BsaHI Bsp1286I BspMI BsrFI
BstAPI BstEII BstXI BstYI BtsI DraIII EaeI EarI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NarI NdeI NsiI
NspI PflMI PpuMI PsrI PvuII SalI SfcI SfoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BciVI BglI BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoICRI EcoNI EcoRV FseI FspI FspAI HpaI KpnI
MfeI MluI NaeI NcoI NgoMIV NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PstI PvuI RsrII SacI SacII
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI