Restriction map for
pF1KB3654
(Linear) (Six-base) MAPSORT of: KIBB3654.seq Check: 1990 from: 1 to: 3087
KIBB3654.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 665 1112 1582 2002 2422 2463 3087
Size: 584 81 447 470 420 420 41 624
Fragments arranged by size:
624 584 470 447 420 420 81 41
AleI CACnn'nnGTG
Cuts at: 0 1178 2354 3087
Size: 1178 1176 733
Fragments arranged by size:
1178 1176 733
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 438 470 3087
Size: 438 32 2617
Fragments arranged by size:
2617 438 32
AlwNI CAG_nnn'CTG
Cuts at: 0 243 270 3087
Size: 243 27 2817
Fragments arranged by size:
2817 243 27
ApaI G_GGCC'C
Cuts at: 0 706 3087
Size: 706 2381
ApoI r'AATT_y
Cuts at: 0 491 2429 2597 2829 2849 2980 3034 3087
Size: 491 1938 168 232 20 131 54 53
Fragments arranged by size:
1938 491 232 168 131 54 53 20
AseI AT'TA_AT
Cuts at: 0 607 3087
Size: 607 2480
BamHI G'GATC_C
Cuts at: 0 537 1337 2513 3087
Size: 537 800 1176 574
Fragments arranged by size:
1176 800 574 537
BanI G'GyrC_C
Cuts at: 0 1421 3087
Size: 1421 1666
BanII G_rGCy'C
Cuts at: 0 706 3087
Size: 706 2381
BbeI G_GCGC'C
Cuts at: 0 1425 3087
Size: 1425 1662
BbsI GAAGACnn'nnnn_
Cuts at: 0 1461 2049 2469 3087
Size: 1461 588 420 618
Fragments arranged by size:
1461 618 588 420
BbvCI CC'TCA_GC
Cuts at: 0 542 3087
Size: 542 2545
BfrBI ATG'CAT
Cuts at: 0 1245 3087
Size: 1245 1842
Bme1580I G_kGCm'C
Cuts at: 0 441 706 3087
Size: 441 265 2381
Fragments arranged by size:
2381 441 265
BmrI ACTGGGnnnn_n'
Cuts at: 0 735 1355 2783 3087
Size: 735 620 1428 304
Fragments arranged by size:
1428 735 620 304
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2044 3054 3087
Size: 2044 1010 33
Fragments arranged by size:
2044 1010 33
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 280 406 517 760 2039 2711 3087
Size: 265 15 126 111 243 1279 672 376
Fragments arranged by size:
1279 672 376 265 243 126 111 15
Bpu10I CC'TnA_GC
Cuts at: 0 178 187 542 3087
Size: 178 9 355 2545
Fragments arranged by size:
2545 355 178 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 109 207 769 1431 3087
Size: 109 98 562 662 1656
Fragments arranged by size:
1656 662 562 109 98
BsaAI yAC'GTr
Cuts at: 0 2567 3087
Size: 2567 520
BsaHI Gr'CG_yC
Cuts at: 0 1422 3087
Size: 1422 1665
BsaWI w'CCGG_w
Cuts at: 0 2269 3087
Size: 2269 818
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 441 471 605 635 3087
Size: 441 30 134 30 2452
Fragments arranged by size:
2452 441 134 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 93 346 367 673 808 1732 3087
Size: 93 253 21 306 135 924 1355
Fragments arranged by size:
1355 924 306 253 135 93 21
BseYI C'CCAG_C
Cuts at: 0 632 3087
Size: 632 2455
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1564 1703 3087
Size: 1564 139 1384
Fragments arranged by size:
1564 1384 139
BsmI GAATG_Cn'
Cuts at: 0 523 1485 2293 2771 3087
Size: 523 962 808 478 316
Fragments arranged by size:
962 808 523 478 316
Bsp1286I G_dGCh'C
Cuts at: 0 441 706 3087
Size: 441 265 2381
Fragments arranged by size:
2381 441 265
BsrBI CCG'CTC
Cuts at: 0 2489 3087
Size: 2489 598
BsrDI GCAATG_nn'
Cuts at: 0 2466 3087
Size: 2466 621
BsrFI r'CCGG_y
Cuts at: 0 182 203 3087
Size: 182 21 2884
Fragments arranged by size:
2884 182 21
BsrGI T'GTAC_A
Cuts at: 0 2250 2754 2922 3087
Size: 2250 504 168 165
Fragments arranged by size:
2250 504 168 165
BssSI C'ACGA_G
Cuts at: 0 2436 3087
Size: 2436 651
BstBI TT'CG_AA
Cuts at: 0 1728 3087
Size: 1728 1359
BstXI CCAn_nnnn'nTGG
Cuts at: 0 319 3087
Size: 319 2768
BstYI r'GATC_y
Cuts at: 0 239 537 1337 2513 3087
Size: 239 298 800 1176 574
Fragments arranged by size:
1176 800 574 298 239
BtsI GCAGTG_nn'
Cuts at: 0 2298 3087
Size: 2298 789
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2448 2483 3087
Size: 2448 35 604
Fragments arranged by size:
2448 604 35
EaeI y'GGCC_r
Cuts at: 0 2838 3087
Size: 2838 249
EarI CTCTTCn'nnn_
Cuts at: 0 581 890 1561 1981 2298 3087
Size: 581 309 671 420 317 789
Fragments arranged by size:
789 671 581 420 317 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 280 406 517 584 665 760 1112
Size: 265 15 126 111 67 81 95 352
Cuts at: 1112 1582 2002 2039 2422 2463 2711 3087
Size: 470 420 37 383 41 248 376
Fragments arranged by size:
470 420 383 376 352 265 248 126
111 95 81 67 41 37 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 49 349 403 580 3087
Size: 49 300 54 177 2507
Fragments arranged by size:
2507 300 177 54 49
EcoO109I rG'GnC_Cy
Cuts at: 0 42 716 879 3087
Size: 42 674 163 2208
Fragments arranged by size:
2208 674 163 42
EcoRI G'AATT_C
Cuts at: 0 2429 2597 2849 2980 3034 3087
Size: 2429 168 252 131 54 53
Fragments arranged by size:
2429 252 168 131 54 53
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 61 991 1794 1839 3087
Size: 61 930 803 45 1248
Fragments arranged by size:
1248 930 803 61 45
HaeII r_GCGC'y
Cuts at: 0 1425 3087
Size: 1425 1662
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 649 681 787 819 1626 1638 1658 1670
Size: 649 32 106 32 807 12 20 12
Cuts at: 1670 2574 2606 3087
Size: 904 32 481
Fragments arranged by size:
904 807 649 481 106 32 32 32
20 12 12
HindIII A'AGCT_T
Cuts at: 0 566 1550 3087
Size: 566 984 1537
Fragments arranged by size:
1537 984 566
KasI G'GCGC_C
Cuts at: 0 1421 3087
Size: 1421 1666
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3020 3087
Size: 3020 67
MscI TGG'CCA
Cuts at: 0 2840 3087
Size: 2840 247
MslI CAynn'nnrTG
Cuts at: 0 1178 2118 2354 2522 2942 3087
Size: 1178 940 236 168 420 145
Fragments arranged by size:
1178 940 420 236 168 145
MspA1I CmG'CkG
Cuts at: 0 487 3087
Size: 487 2600
NaeI GCC'GGC
Cuts at: 0 184 3087
Size: 184 2903
NarI GG'CG_CC
Cuts at: 0 1422 3087
Size: 1422 1665
NdeI CA'TA_TG
Cuts at: 0 2115 3087
Size: 2115 972
NgoMIV G'CCGG_C
Cuts at: 0 182 3087
Size: 182 2905
NsiI A_TGCA'T
Cuts at: 0 1247 3087
Size: 1247 1840
NspI r_CATG'y
Cuts at: 0 698 3087
Size: 698 2389
PflMI CCAn_nnn'nTGG
Cuts at: 0 429 645 3087
Size: 429 216 2442
Fragments arranged by size:
2442 429 216
PfoI T'CCnGG_A
Cuts at: 0 242 533 3087
Size: 242 291 2554
Fragments arranged by size:
2554 291 242
PpuMI rG'GwC_Cy
Cuts at: 0 879 3087
Size: 879 2208
PshAI GACnn'nnGTC
Cuts at: 0 2732 3087
Size: 2732 355
PsiI TTA'TAA
Cuts at: 0 1109 1156 1277 1361 1445 1529 2453 2705
Size: 1109 47 121 84 84 84 924 252
Cuts at: 2705 3087
Size: 382
Fragments arranged by size:
1109 924 382 252 121 84 84 84
47
PspOMI G'GGCC_C
Cuts at: 0 702 3087
Size: 702 2385
PstI C_TGCA'G
Cuts at: 0 362 3087
Size: 362 2725
PvuII CAG'CTG
Cuts at: 0 487 3087
Size: 487 2600
SapI GCTCTTCn'nnn_
Cuts at: 0 1561 1981 2298 3087
Size: 1561 420 317 789
Fragments arranged by size:
1561 789 420 317
SfcI C'TryA_G
Cuts at: 0 358 1389 3087
Size: 358 1031 1698
Fragments arranged by size:
1698 1031 358
SfoI GGC'GCC
Cuts at: 0 1423 3087
Size: 1423 1664
SgrAI Cr'CCGG_yG
Cuts at: 0 203 3087
Size: 203 2884
SnaBI TAC'GTA
Cuts at: 0 2567 3087
Size: 2567 520
StuI AGG'CCT
Cuts at: 0 1403 3087
Size: 1403 1684
StyI C'CwwG_G
Cuts at: 0 90 230 3087
Size: 90 140 2857
Fragments arranged by size:
2857 140 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 799 2864 3087
Size: 799 2065 223
Fragments arranged by size:
2065 799 223
TatI w'GTAC_w
Cuts at: 0 462 722 2250 2754 2922 3087
Size: 462 260 1528 504 168 165
Fragments arranged by size:
1528 504 462 260 168 165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 605 637 3087
Size: 605 32 2450
Fragments arranged by size:
2450 605 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 157 3087
Size: 157 2930
XmnI GAAnn'nnTTC
Cuts at: 0 1468 1552 1804 2224 2476 3087
Size: 1468 84 252 420 252 611
Fragments arranged by size:
1468 611 420 252 252 84
Enzymes that do cut:
AcuI AleI AloI AlwNI ApaI ApoI AseI BamHI
BanI BanII BbeI BbsI BbvCI BfrBI Bme1580I BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsmI Bsp1286I BsrBI BsrDI BsrFI
BsrGI BssSI BstBI BstXI BstYI BtsI CspCI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HindIII KasI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PflMI PfoI PpuMI PshAI
PsiI PspOMI PstI PvuII SapI SfcI SfoI SgrAI
SnaBI StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI ApaLI AscI AsiSI AvaI AvrII
BaeI BcgI BciVI BclI BglI BglII BlpI BmgBI
BmtI BpuEI BsaBI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BspMI BssHII BstAPI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI
EcoRV FseI FspI FspAI HincII HpaI KpnI MfeI
MluI NcoI NheI NotI NruI PacI PasI PciI
PmeI PmlI PpiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SmaI SmlI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI ZraI