Restriction map for
pF1KB3650
(Linear) (Six-base) MAPSORT of: KIBB3650.seq Check: 7623 from: 1 to: 1434
KIBB3650.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AccI GT'mk_AC
Cuts at: 0 812 1434
Size: 812 622
Acc65I G'GTAC_C
Cuts at: 0 572 982 1434
Size: 572 410 452
Fragments arranged by size:
572 452 410
AhdI GACnn_n'nnGTC
Cuts at: 0 63 1434
Size: 63 1371
AleI CACnn'nnGTG
Cuts at: 0 1157 1434
Size: 1157 277
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 832 864 1434
Size: 832 32 570
Fragments arranged by size:
832 570 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1423 1434
Size: 1423 11
ApaI G_GGCC'C
Cuts at: 0 286 613 1434
Size: 286 327 821
Fragments arranged by size:
821 327 286
BanI G'GyrC_C
Cuts at: 0 3 382 572 982 1077 1434
Size: 3 379 190 410 95 357
Fragments arranged by size:
410 379 357 190 95 3
BanII G_rGCy'C
Cuts at: 0 128 286 613 1434
Size: 128 158 327 821
Fragments arranged by size:
821 327 158 128
BbeI G_GCGC'C
Cuts at: 0 386 1434
Size: 386 1048
BbsI GAAGACnn'nnnn_
Cuts at: 0 658 1299 1434
Size: 658 641 135
Fragments arranged by size:
658 641 135
BbvCI CC'TCA_GC
Cuts at: 0 440 1434
Size: 440 994
BglI GCCn_nnn'nGGC
Cuts at: 0 37 1434
Size: 37 1397
Bme1580I G_kGCm'C
Cuts at: 0 286 613 1434
Size: 286 327 821
Fragments arranged by size:
821 327 286
BmrI ACTGGGnnnn_n'
Cuts at: 0 306 846 857 990 1434
Size: 306 540 11 133 444
Fragments arranged by size:
540 444 306 133 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 147 1434
Size: 147 1287
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 1200 1340 1434
Size: 289 911 140 94
Fragments arranged by size:
911 289 140 94
Bpu10I CC'TnA_GC
Cuts at: 0 329 440 1434
Size: 329 111 994
Fragments arranged by size:
994 329 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 484 1434
Size: 393 91 950
Fragments arranged by size:
950 393 91
BsaI GGTCTCn'nnnn_
Cuts at: 0 6 412 429 1434
Size: 6 406 17 1005
Fragments arranged by size:
1005 406 17 6
BsaAI yAC'GTr
Cuts at: 0 1199 1434
Size: 1199 235
BsaHI Gr'CG_yC
Cuts at: 0 383 485 1434
Size: 383 102 949
Fragments arranged by size:
949 383 102
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 205 235 448 478 832 862 1159 1189
Size: 205 30 213 30 354 30 297 30
Cuts at: 1189 1372 1402 1434
Size: 183 30 32
Fragments arranged by size:
354 297 213 205 183 32 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 259 469 472 886 1237 1434
Size: 259 210 3 414 351 197
Fragments arranged by size:
414 351 259 210 197 3
BseYI C'CCAG_C
Cuts at: 0 447 935 1407 1416 1434
Size: 447 488 472 9 18
Fragments arranged by size:
488 472 447 18 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 135 486 1434
Size: 66 69 351 948
Fragments arranged by size:
948 351 69 66
BsiHKAI G_wGCw'C
Cuts at: 0 128 1434
Size: 128 1306
Bsp1286I G_dGCh'C
Cuts at: 0 128 286 613 1434
Size: 128 158 327 821
Fragments arranged by size:
821 327 158 128
BsrBI CCG'CTC
Cuts at: 0 807 1434
Size: 807 627
BsrFI r'CCGG_y
Cuts at: 0 1181 1434
Size: 1181 253
BstAPI GCAn_nnn'nTGC
Cuts at: 0 46 1023 1434
Size: 46 977 411
Fragments arranged by size:
977 411 46
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1159 1434
Size: 1159 275
Bsu36I CC'TnA_GG
Cuts at: 0 353 374 395 1434
Size: 353 21 21 1039
Fragments arranged by size:
1039 353 21 21
BtgI C'CryG_G
Cuts at: 0 1046 1158 1434
Size: 1046 112 276
Fragments arranged by size:
1046 276 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1102 1377 1434
Size: 1102 275 57
Fragments arranged by size:
1102 275 57
BtsI GCAGTG_nn'
Cuts at: 0 474 566 1434
Size: 474 92 868
Fragments arranged by size:
868 474 92
ClaI AT'CG_AT
Cuts at: 0 617 1434
Size: 617 817
DraIII CAC_nnn'GTG
Cuts at: 0 1023 1434
Size: 1023 411
EaeI y'GGCC_r
Cuts at: 0 798 1053 1094 1161 1183 1425 1434
Size: 798 255 41 67 22 242 9
Fragments arranged by size:
798 255 242 67 41 22 9
EarI CTCTTCn'nnn_
Cuts at: 0 353 667 964 1434
Size: 353 314 297 470
Fragments arranged by size:
470 353 314 297
EcoICRI GAG'CTC
Cuts at: 0 126 1434
Size: 126 1308
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 1200 1340 1434
Size: 289 911 140 94
Fragments arranged by size:
911 289 140 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 664 1434
Size: 664 770
EcoO109I rG'GnC_Cy
Cuts at: 0 282 283 363 609 610 1434
Size: 282 1 80 246 1 824
Fragments arranged by size:
824 282 246 80 1 1
HaeII r_GCGC'y
Cuts at: 0 386 687 1434
Size: 386 301 747
Fragments arranged by size:
747 386 301
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 5 37 49 81 411 443 1434
Size: 5 32 12 32 330 32 991
Fragments arranged by size:
991 330 32 32 32 12 5
KasI G'GCGC_C
Cuts at: 0 382 1434
Size: 382 1052
KpnI G_GTAC'C
Cuts at: 0 576 986 1434
Size: 576 410 448
Fragments arranged by size:
576 448 410
MscI TGG'CCA
Cuts at: 0 1096 1427 1434
Size: 1096 331 7
Fragments arranged by size:
1096 331 7
MslI CAynn'nnrTG
Cuts at: 0 1157 1434
Size: 1157 277
MspA1I CmG'CkG
Cuts at: 0 80 927 935 1434
Size: 80 847 8 499
Fragments arranged by size:
847 499 80 8
NaeI GCC'GGC
Cuts at: 0 1183 1434
Size: 1183 251
NarI GG'CG_CC
Cuts at: 0 383 1434
Size: 383 1051
NcoI C'CATG_G
Cuts at: 0 1046 1434
Size: 1046 388
NgoMIV G'CCGG_C
Cuts at: 0 1181 1434
Size: 1181 253
PflMI CCAn_nnn'nTGG
Cuts at: 0 1423 1434
Size: 1423 11
PfoI T'CCnGG_A
Cuts at: 0 701 749 1434
Size: 701 48 685
Fragments arranged by size:
701 685 48
PmlI CAC'GTG
Cuts at: 0 1199 1434
Size: 1199 235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 832 864 1434
Size: 832 32 570
Fragments arranged by size:
832 570 32
PspOMI G'GGCC_C
Cuts at: 0 282 609 1434
Size: 282 327 825
Fragments arranged by size:
825 327 282
PvuII CAG'CTG
Cuts at: 0 935 1434
Size: 935 499
SacI G_AGCT'C
Cuts at: 0 128 1434
Size: 128 1306
ScaI AGT'ACT
Cuts at: 0 535 679 835 1330 1402 1434
Size: 535 144 156 495 72 32
Fragments arranged by size:
535 495 156 144 72 32
SfcI C'TryA_G
Cuts at: 0 792 1257 1434
Size: 792 465 177
Fragments arranged by size:
792 465 177
SfoI GGC'GCC
Cuts at: 0 384 1434
Size: 384 1050
SmlI C'TyrA_G
Cuts at: 0 408 499 1434
Size: 408 91 935
Fragments arranged by size:
935 408 91
StuI AGG'CCT
Cuts at: 0 414 567 1434
Size: 414 153 867
Fragments arranged by size:
867 414 153
StyI C'CwwG_G
Cuts at: 0 770 1046 1091 1434
Size: 770 276 45 343
Fragments arranged by size:
770 343 276 45
TatI w'GTAC_w
Cuts at: 0 221 533 677 833 1328 1400 1434
Size: 221 312 144 156 495 72 34
Fragments arranged by size:
495 312 221 156 144 72 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 94 126 448 480 1434
Size: 94 32 322 32 954
Fragments arranged by size:
954 322 94 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 793 1434
Size: 793 641
XmnI GAAnn'nnTTC
Cuts at: 0 478 1434
Size: 478 956
Enzymes that do cut:
AccI Acc65I AhdI AleI AloI AlwNI ApaI BanI
BanII BbeI BbsI BbvCI BglI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrFI BstAPI BstXI
Bsu36I BtgI BtgZI BtsI ClaI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV PflMI
PfoI PmlI PpiI PspOMI PvuII SacI ScaI SfcI
SfoI SmlI StuI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AlfI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I CspCI DraI DrdI EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MmeI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XbaI XhoI XmaI ZraI