Restriction map for
pF1KB3644
(Linear) (Six-base) MAPSORT of: KIBB3644.seq Check: 6832 from: 1 to: 1137
KIBB3644.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 945 1137
Size: 945 192
AatII G_ACGT'C
Cuts at: 0 7 1137
Size: 7 1130
AccI GT'mk_AC
Cuts at: 0 719 1137
Size: 719 418
Acc65I G'GTAC_C
Cuts at: 0 487 1137
Size: 487 650
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1137
Size: 637 500
AfeI AGC'GCT
Cuts at: 0 142 1137
Size: 142 995
AlwNI CAG_nnn'CTG
Cuts at: 0 730 1137
Size: 730 407
ApaI G_GGCC'C
Cuts at: 0 67 1137
Size: 67 1070
ApaLI G'TGCA_C
Cuts at: 0 334 1137
Size: 334 803
BanI G'GyrC_C
Cuts at: 0 487 1137
Size: 487 650
BanII G_rGCy'C
Cuts at: 0 67 1137
Size: 67 1070
BciVI GTATCCnnnnn_n'
Cuts at: 0 65 1137
Size: 65 1072
BglII A'GATC_T
Cuts at: 0 798 1137
Size: 798 339
Bme1580I G_kGCm'C
Cuts at: 0 67 338 1113 1137
Size: 67 271 775 24
Fragments arranged by size:
775 271 67 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 373 1137
Size: 373 764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 368 1137
Size: 310 58 769
Fragments arranged by size:
769 310 58
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 1048 1137
Size: 139 909 89
Fragments arranged by size:
909 139 89
BsaI GGTCTCn'nnnn_
Cuts at: 0 523 775 1043 1137
Size: 523 252 268 94
Fragments arranged by size:
523 268 252 94
BsaHI Gr'CG_yC
Cuts at: 0 4 1137
Size: 4 1133
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 610 922 1087 1137
Size: 610 312 165 50
Fragments arranged by size:
610 312 165 50
BseYI C'CCAG_C
Cuts at: 0 446 1137
Size: 446 691
BsiHKAI G_wGCw'C
Cuts at: 0 128 338 458 1137
Size: 128 210 120 679
Fragments arranged by size:
679 210 128 120
Bsp1286I G_dGCh'C
Cuts at: 0 67 128 338 458 1113 1137
Size: 67 61 210 120 655 24
Fragments arranged by size:
655 210 120 67 61 24
BspHI T'CATG_A
Cuts at: 0 587 1137
Size: 587 550
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 293 908 945 1137
Size: 293 615 37 192
Fragments arranged by size:
615 293 192 37
BsrDI GCAATG_nn'
Cuts at: 0 128 1137
Size: 128 1009
BsrFI r'CCGG_y
Cuts at: 0 50 1137
Size: 50 1087
BstAPI GCAn_nnn'nTGC
Cuts at: 0 581 1137
Size: 581 556
BstXI CCAn_nnnn'nTGG
Cuts at: 0 468 1137
Size: 468 669
BstYI r'GATC_y
Cuts at: 0 798 911 1137
Size: 798 113 226
Fragments arranged by size:
798 226 113
BtsI GCAGTG_nn'
Cuts at: 0 193 819 1113 1137
Size: 193 626 294 24
Fragments arranged by size:
626 294 193 24
DrdI GACnn_nn'nnGTC
Cuts at: 0 861 1137
Size: 861 276
EaeI y'GGCC_r
Cuts at: 0 305 425 1137
Size: 305 120 712
Fragments arranged by size:
712 305 120
EarI CTCTTCn'nnn_
Cuts at: 0 214 364 812 1137
Size: 214 150 448 325
Fragments arranged by size:
448 325 214 150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 96 1137
Size: 96 1041
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 368 637 1137
Size: 310 58 269 500
Fragments arranged by size:
500 310 269 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 904 1137
Size: 904 233
EcoO109I rG'GnC_Cy
Cuts at: 0 159 282 1137
Size: 159 123 855
Fragments arranged by size:
855 159 123
FspI TGC'GCA
Cuts at: 0 610 889 1123 1137
Size: 610 279 234 14
Fragments arranged by size:
610 279 234 14
FspAI rTGC'GCAy
Cuts at: 0 889 1123 1137
Size: 889 234 14
Fragments arranged by size:
889 234 14
HaeII r_GCGC'y
Cuts at: 0 144 195 1137
Size: 144 51 942
Fragments arranged by size:
942 144 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 712 744 1137
Size: 712 32 393
Fragments arranged by size:
712 393 32
HindIII A'AGCT_T
Cuts at: 0 199 1137
Size: 199 938
KpnI G_GTAC'C
Cuts at: 0 491 1137
Size: 491 646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1049 1137
Size: 1049 88
MscI TGG'CCA
Cuts at: 0 427 1137
Size: 427 710
MslI CAynn'nnrTG
Cuts at: 0 181 1137
Size: 181 956
MspA1I CmG'CkG
Cuts at: 0 450 1137
Size: 450 687
NaeI GCC'GGC
Cuts at: 0 52 1137
Size: 52 1085
NgoMIV G'CCGG_C
Cuts at: 0 50 1137
Size: 50 1087
NspI r_CATG'y
Cuts at: 0 186 553 1137
Size: 186 367 584
Fragments arranged by size:
584 367 186
PasI CC'CwG_GG
Cuts at: 0 67 1137
Size: 67 1070
PfoI T'CCnGG_A
Cuts at: 0 467 1137
Size: 467 670
PpuMI rG'GwC_Cy
Cuts at: 0 159 282 1137
Size: 159 123 855
Fragments arranged by size:
855 159 123
PspOMI G'GGCC_C
Cuts at: 0 63 1137
Size: 63 1074
PvuII CAG'CTG
Cuts at: 0 450 1137
Size: 450 687
SapI GCTCTTCn'nnn_
Cuts at: 0 214 1137
Size: 214 923
SexAI A'CCwGG_T
Cuts at: 0 695 1137
Size: 695 442
SmlI C'TyrA_G
Cuts at: 0 154 1063 1137
Size: 154 909 74
Fragments arranged by size:
909 154 74
SphI G_CATG'C
Cuts at: 0 553 1137
Size: 553 584
StyI C'CwwG_G
Cuts at: 0 14 668 1137
Size: 14 654 469
Fragments arranged by size:
654 469 14
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 382 1137
Size: 382 755
ZraI GAC'GTC
Cuts at: 0 5 1137
Size: 5 1132
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AlwNI ApaI
ApaLI BanI BanII BciVI BglII Bme1580I BplI BpmI
BpuEI BsaI BsaHI BseRI BseYI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BsrFI BstAPI BstXI BstYI BtsI DrdI
EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI FspAI
HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I
NaeI NgoMIV NspI PasI PfoI PpuMI PspOMI PvuII
SapI SexAI SmlI SphI StyI XcmI ZraI
Enzymes that do not cut:
AclI AflII AflIII AgeI AhdI AleI AlfI AloI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BclI BfrBI BglI BlpI
BmgBI BmrI BmtI Bpu10I BsaAI BsaBI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrGI
BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII EagI EcoICRI EcoRI EcoRV
FalI FseI HincII HpaI KasI MfeI MluI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI SwaI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI