Restriction map for
pF1KB3634
(Linear) (Six-base) MAPSORT of: KIBB3634.seq Check: 935 from: 1 to: 999
KIBB3634.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AleI CACnn'nnGTG
Cuts at: 0 323 430 999
Size: 323 107 569
Fragments arranged by size:
569 323 107
AseI AT'TA_AT
Cuts at: 0 896 999
Size: 896 103
BanI G'GyrC_C
Cuts at: 0 640 999
Size: 640 359
BglII A'GATC_T
Cuts at: 0 195 999
Size: 195 804
BlpI GC'TnA_GC
Cuts at: 0 77 236 999
Size: 77 159 763
Fragments arranged by size:
763 159 77
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 863 999
Size: 350 513 136
Fragments arranged by size:
513 350 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 999
Size: 400 599
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 134 164 999
Size: 134 30 835
Fragments arranged by size:
835 134 30
BseYI C'CCAG_C
Cuts at: 0 862 999
Size: 862 137
BsrBI CCG'CTC
Cuts at: 0 10 999
Size: 10 989
BsrDI GCAATG_nn'
Cuts at: 0 43 404 999
Size: 43 361 595
Fragments arranged by size:
595 361 43
BsrGI T'GTAC_A
Cuts at: 0 248 463 999
Size: 248 215 536
Fragments arranged by size:
536 248 215
BstAPI GCAn_nnn'nTGC
Cuts at: 0 454 736 999
Size: 454 282 263
Fragments arranged by size:
454 282 263
BstXI CCAn_nnnn'nTGG
Cuts at: 0 651 999
Size: 651 348
BstYI r'GATC_y
Cuts at: 0 195 999
Size: 195 804
BtgI C'CryG_G
Cuts at: 0 504 999
Size: 504 495
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 120 155 999
Size: 120 35 844
Fragments arranged by size:
844 120 35
DraI TTT'AAA
Cuts at: 0 113 999
Size: 113 886
EarI CTCTTCn'nnn_
Cuts at: 0 670 999
Size: 670 329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 863 999
Size: 350 513 136
Fragments arranged by size:
513 350 136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 277 999
Size: 277 722
MfeI C'AATT_G
Cuts at: 0 884 999
Size: 884 115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 768 813 876 999
Size: 768 45 63 123
Fragments arranged by size:
768 123 63 45
MslI CAynn'nnrTG
Cuts at: 0 86 323 430 999
Size: 86 237 107 569
Fragments arranged by size:
569 237 107 86
NspI r_CATG'y
Cuts at: 0 85 727 783 999
Size: 85 642 56 216
Fragments arranged by size:
642 216 85 56
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 534 566 999
Size: 534 32 433
Fragments arranged by size:
534 433 32
PstI C_TGCA'G
Cuts at: 0 129 528 732 999
Size: 129 399 204 267
Fragments arranged by size:
399 267 204 129
ScaI AGT'ACT
Cuts at: 0 570 999
Size: 570 429
SfcI C'TryA_G
Cuts at: 0 125 524 728 999
Size: 125 399 204 271
Fragments arranged by size:
399 271 204 125
SmlI C'TyrA_G
Cuts at: 0 379 999
Size: 379 620
SphI G_CATG'C
Cuts at: 0 85 999
Size: 85 914
StuI AGG'CCT
Cuts at: 0 257 999
Size: 257 742
StyI C'CwwG_G
Cuts at: 0 138 999
Size: 138 861
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 117 999
Size: 117 882
TatI w'GTAC_w
Cuts at: 0 248 463 568 999
Size: 248 215 105 431
Fragments arranged by size:
431 248 215 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 134 166 999
Size: 134 32 833
Fragments arranged by size:
833 134 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 859 999
Size: 859 140
XmnI GAAnn'nnTTC
Cuts at: 0 304 999
Size: 304 695
Enzymes that do cut:
AleI AseI BanI BglII BlpI BpmI BpuEI BsaXI
BseYI BsrBI BsrDI BsrGI BstAPI BstXI BstYI BtgI
CspCI DraI EarI Eco57MI FalI MfeI MmeI MslI
NspI PsrI PstI ScaI SfcI SmlI SphI StuI
StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AsiSI AvaI AvrII BaeI BamHI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MluI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XhoI XmaI ZraI