Restriction map for
pF1KB3626
(Linear) (Six-base) MAPSORT of: KIBB3626.seq Check: 4956 from: 1 to: 3462
KIBB3626.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1447 1449 2700 3462
Size: 1447 2 1251 762
Fragments arranged by size:
1447 1251 762 2
AatII G_ACGT'C
Cuts at: 0 3191 3462
Size: 3191 271
AccI GT'mk_AC
Cuts at: 0 1700 3462
Size: 1700 1762
AclI AA'CG_TT
Cuts at: 0 926 3462
Size: 926 2536
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 817 1042 1201 1856 2609 2749 3462
Size: 817 225 159 655 753 140 713
Fragments arranged by size:
817 753 713 655 225 159 140
AfeI AGC'GCT
Cuts at: 0 2617 3462
Size: 2617 845
AflII C'TTAA_G
Cuts at: 0 2784 2890 3462
Size: 2784 106 572
Fragments arranged by size:
2784 572 106
AhdI GACnn_n'nnGTC
Cuts at: 0 300 3192 3462
Size: 300 2892 270
Fragments arranged by size:
2892 300 270
AleI CACnn'nnGTG
Cuts at: 0 1276 3462
Size: 1276 2186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 216 3462
Size: 216 3246
AlwNI CAG_nnn'CTG
Cuts at: 0 1590 2587 3462
Size: 1590 997 875
Fragments arranged by size:
1590 997 875
ApoI r'AATT_y
Cuts at: 0 748 2071 2869 3169 3397 3462
Size: 748 1323 798 300 228 65
Fragments arranged by size:
1323 798 748 300 228 65
AseI AT'TA_AT
Cuts at: 0 2534 3462
Size: 2534 928
AvaI C'yCGr_G
Cuts at: 0 1643 3462
Size: 1643 1819
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 451 484 3462
Size: 451 33 2978
Fragments arranged by size:
2978 451 33
BamHI G'GATC_C
Cuts at: 0 1730 2630 3462
Size: 1730 900 832
Fragments arranged by size:
1730 900 832
BanI G'GyrC_C
Cuts at: 0 1468 3462
Size: 1468 1994
BanII G_rGCy'C
Cuts at: 0 64 140 2672 3462
Size: 64 76 2532 790
Fragments arranged by size:
2532 790 76 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 76 2843 3462
Size: 76 2767 619
Fragments arranged by size:
2767 619 76
BbvCI CC'TCA_GC
Cuts at: 0 1393 3462
Size: 1393 2069
BfrBI ATG'CAT
Cuts at: 0 2255 3462
Size: 2255 1207
BglI GCCn_nnn'nGGC
Cuts at: 0 144 3462
Size: 144 3318
BglII A'GATC_T
Cuts at: 0 2780 3462
Size: 2780 682
Bme1580I G_kGCm'C
Cuts at: 0 1473 2103 3349 3462
Size: 1473 630 1246 113
Fragments arranged by size:
1473 1246 630 113
BmgBI CAC'GTC
Cuts at: 0 1278 1980 3462
Size: 1278 702 1482
Fragments arranged by size:
1482 1278 702
BmrI ACTGGGnnnn_n'
Cuts at: 0 962 3462
Size: 962 2500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 484 3131 3462
Size: 106 378 2647 331
Fragments arranged by size:
2647 378 331 106
Bpu10I CC'TnA_GC
Cuts at: 0 95 479 1393 2104 3462
Size: 95 384 914 711 1358
Fragments arranged by size:
1358 914 711 384 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1561 1720 3462
Size: 1561 159 1742
Fragments arranged by size:
1742 1561 159
BsaI GGTCTCn'nnnn_
Cuts at: 0 515 1553 1938 3462
Size: 515 1038 385 1524
Fragments arranged by size:
1524 1038 515 385
BsaAI yAC'GTr
Cuts at: 0 1389 3462
Size: 1389 2073
BsaBI GATnn'nnATC
Cuts at: 0 1256 3462
Size: 1256 2206
BsaHI Gr'CG_yC
Cuts at: 0 381 1964 3188 3462
Size: 381 1583 1224 274
Fragments arranged by size:
1583 1224 381 274
BsaWI w'CCGG_w
Cuts at: 0 320 2039 3462
Size: 320 1719 1423
Fragments arranged by size:
1719 1423 320
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 457 487 1192 1222 1369 1399 3462
Size: 457 30 705 30 147 30 2063
Fragments arranged by size:
2063 705 457 147 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 72 203 1075 1213 1390 2149 3462
Size: 72 131 872 138 177 759 1313
Fragments arranged by size:
1313 872 759 177 138 131 72
BseYI C'CCAG_C
Cuts at: 0 147 1549 1876 3392 3462
Size: 147 1402 327 1516 70
Fragments arranged by size:
1516 1402 327 147 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 1254 1389 1483 1510 1525 1771 2448
Size: 176 1078 135 94 27 15 246 677
Cuts at: 2448 2814 3462
Size: 366 648
Fragments arranged by size:
1078 677 648 366 246 176 135 94
27 15
BsiEI CG_ry'CG
Cuts at: 0 1728 3462
Size: 1728 1734
BsiHKAI G_wGCw'C
Cuts at: 0 64 1763 2672 3462
Size: 64 1699 909 790
Fragments arranged by size:
1699 909 790 64
BsmI GAATG_Cn'
Cuts at: 0 2257 3462
Size: 2257 1205
Bsp1286I G_dGCh'C
Cuts at: 0 64 140 1473 1763 2103 2672 3349 3462
Size: 64 76 1333 290 340 569 677 113
Fragments arranged by size:
1333 677 569 340 290 113 76 64
BspHI T'CATG_A
Cuts at: 0 1427 1565 2468 2522 2799 3462
Size: 1427 138 903 54 277 663
Fragments arranged by size:
1427 903 663 277 138 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1447 1449 1667 2666 2700 3462
Size: 1447 2 218 999 34 762
Fragments arranged by size:
1447 999 762 218 34 2
BsrDI GCAATG_nn'
Cuts at: 0 36 3462
Size: 36 3426
BsrFI r'CCGG_y
Cuts at: 0 2423 3462
Size: 2423 1039
BsrGI T'GTAC_A
Cuts at: 0 1333 3462
Size: 1333 2129
BssSI C'ACGA_G
Cuts at: 0 1195 1776 3462
Size: 1195 581 1686
Fragments arranged by size:
1686 1195 581
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1590 3219 3462
Size: 1590 1629 243
Fragments arranged by size:
1629 1590 243
BstBI TT'CG_AA
Cuts at: 0 910 3462
Size: 910 2552
BstEII G'GTnAC_C
Cuts at: 0 2746 3462
Size: 2746 716
BstXI CCAn_nnnn'nTGG
Cuts at: 0 462 1662 2091 3462
Size: 462 1200 429 1371
Fragments arranged by size:
1371 1200 462 429
BstYI r'GATC_y
Cuts at: 0 1073 1586 1730 1743 2025 2372 2630 2780
Size: 1073 513 144 13 282 347 258 150
Cuts at: 2780 3462
Size: 682
Fragments arranged by size:
1073 682 513 347 282 258 150 144
13
BtgI C'CryG_G
Cuts at: 0 35 2065 2133 2269 3251 3285 3462
Size: 35 2030 68 136 982 34 177
Fragments arranged by size:
2030 982 177 136 68 35 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2063 3462
Size: 2063 1399
BtsI GCAGTG_nn'
Cuts at: 0 159 3462
Size: 159 3303
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2573 2589 2608 2624 3462
Size: 2573 16 19 16 838
Fragments arranged by size:
2573 838 19 16 16
DraI TTT'AAA
Cuts at: 0 3458 3462
Size: 3458 4
DraIII CAC_nnn'GTG
Cuts at: 0 1950 3048 3462
Size: 1950 1098 414
Fragments arranged by size:
1950 1098 414
EaeI y'GGCC_r
Cuts at: 0 584 2093 3288 3462
Size: 584 1509 1195 174
Fragments arranged by size:
1509 1195 584 174
EarI CTCTTCn'nnn_
Cuts at: 0 1076 1194 1832 1994 2035 2540 3462
Size: 1076 118 638 162 41 505 922
Fragments arranged by size:
1076 922 638 505 162 118 41
EcoICRI GAG'CTC
Cuts at: 0 62 2670 3462
Size: 62 2608 792
Fragments arranged by size:
2608 792 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 484 817 1042 1201 1856 2609 2749
Size: 106 378 333 225 159 655 753 140
Cuts at: 2749 3131 3462
Size: 382 331
Fragments arranged by size:
753 655 382 378 333 331 225 159
140 106
EcoNI CCTnn'n_nnAGG
Cuts at: 0 98 1738 3462
Size: 98 1640 1724
Fragments arranged by size:
1724 1640 98
EcoO109I rG'GnC_Cy
Cuts at: 0 90 1799 2017 3462
Size: 90 1709 218 1445
Fragments arranged by size:
1709 1445 218 90
EcoRI G'AATT_C
Cuts at: 0 3397 3462
Size: 3397 65
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2349 3462
Size: 2349 1113
FspI TGC'GCA
Cuts at: 0 1594 3462
Size: 1594 1868
HaeII r_GCGC'y
Cuts at: 0 2619 3462
Size: 2619 843
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 105 137 286 318 442 474 942 974
Size: 105 32 149 32 124 32 468 32
Cuts at: 974 1192 1224 1710 1742 3178 3210 3462
Size: 218 32 486 32 1436 32 252
Fragments arranged by size:
1436 486 468 252 218 149 124 105
32 32 32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 2539 3420 3462
Size: 2539 881 42
Fragments arranged by size:
2539 881 42
MfeI C'AATT_G
Cuts at: 0 342 3080 3462
Size: 342 2738 382
Fragments arranged by size:
2738 382 342
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 1679 3426 3462
Size: 107 1572 1747 36
Fragments arranged by size:
1747 1572 107 36
MscI TGG'CCA
Cuts at: 0 586 2095 3290 3462
Size: 586 1509 1195 172
Fragments arranged by size:
1509 1195 586 172
MslI CAynn'nnrTG
Cuts at: 0 198 709 842 911 1276 1426 2138 2233
Size: 198 511 133 69 365 150 712 95
Cuts at: 2233 2445 3462
Size: 212 1017
Fragments arranged by size:
1017 712 511 365 212 198 150 133
95 69
MspA1I CmG'CkG
Cuts at: 0 1716 2091 2477 3462
Size: 1716 375 386 985
Fragments arranged by size:
1716 985 386 375
NcoI C'CATG_G
Cuts at: 0 35 2065 2133 2269 3251 3285 3462
Size: 35 2030 68 136 982 34 177
Fragments arranged by size:
2030 982 177 136 68 35 34
NsiI A_TGCA'T
Cuts at: 0 2257 3462
Size: 2257 1205
NspI r_CATG'y
Cuts at: 0 203 3462
Size: 203 3259
PflMI CCAn_nnn'nTGG
Cuts at: 0 260 2066 2348 3286 3462
Size: 260 1806 282 938 176
Fragments arranged by size:
1806 938 282 260 176
PfoI T'CCnGG_A
Cuts at: 0 1076 3462
Size: 1076 2386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1169 1201 3462
Size: 1169 32 2261
Fragments arranged by size:
2261 1169 32
PpuMI rG'GwC_Cy
Cuts at: 0 1799 2017 3462
Size: 1799 218 1445
Fragments arranged by size:
1799 1445 218
PstI C_TGCA'G
Cuts at: 0 2677 3462
Size: 2677 785
PvuI CG_AT'CG
Cuts at: 0 1728 3462
Size: 1728 1734
PvuII CAG'CTG
Cuts at: 0 1716 2091 3462
Size: 1716 375 1371
Fragments arranged by size:
1716 1371 375
RsrII CG'GwC_CG
Cuts at: 0 1536 3462
Size: 1536 1926
SacI G_AGCT'C
Cuts at: 0 64 2672 3462
Size: 64 2608 790
Fragments arranged by size:
2608 790 64
SanDI GG'GwC_CC
Cuts at: 0 1799 3462
Size: 1799 1663
SapI GCTCTTCn'nnn_
Cuts at: 0 2540 3462
Size: 2540 922
SexAI A'CCwGG_T
Cuts at: 0 2126 3462
Size: 2126 1336
SfcI C'TryA_G
Cuts at: 0 1518 2673 3462
Size: 1518 1155 789
Fragments arranged by size:
1518 1155 789
SmlI C'TyrA_G
Cuts at: 0 1576 1735 2784 2890 3462
Size: 1576 159 1049 106 572
Fragments arranged by size:
1576 1049 572 159 106
SphI G_CATG'C
Cuts at: 0 203 3462
Size: 203 3259
SspI AAT'ATT
Cuts at: 0 854 3462
Size: 854 2608
StuI AGG'CCT
Cuts at: 0 1204 2505 3462
Size: 1204 1301 957
Fragments arranged by size:
1301 1204 957
StyI C'CwwG_G
Cuts at: 0 35 635 818 1764 2065 2133 2269 3251
Size: 35 600 183 946 301 68 136 982
Cuts at: 3251 3285 3462
Size: 34 177
Fragments arranged by size:
982 946 600 301 183 177 136 68
35 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 547 3462
Size: 547 2915
TatI w'GTAC_w
Cuts at: 0 161 1333 2146 2816 3462
Size: 161 1172 813 670 646
Fragments arranged by size:
1172 813 670 646 161
Tth111I GACn'n_nGTC
Cuts at: 0 2128 3462
Size: 2128 1334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1662 3052 3462
Size: 1662 1390 410
Fragments arranged by size:
1662 1390 410
XmnI GAAnn'nnTTC
Cuts at: 0 1480 2900 3462
Size: 1480 1420 562
Fragments arranged by size:
1480 1420 562
ZraI GAC'GTC
Cuts at: 0 3189 3462
Size: 3189 273
Enzymes that do cut:
AarI AatII AccI AclI AcuI AfeI AflII AhdI
AleI AlfI AlwNI ApoI AseI AvaI BaeI BamHI
BanI BanII BbsI BbvCI BfrBI BglI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HindIII MfeI
MmeI MscI MslI MspA1I NcoI NsiI NspI PflMI
PfoI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SanDI SapI SexAI SfcI SmlI SphI SspI StuI
StyI TaqII TatI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AflIII AgeI AloI ApaI ApaLI AscI AsiSI
AvrII BbeI BcgI BciVI BclI BlpI BmtI BplI
BsiWI BsmBI BspEI BsrBI BssHII BstZ17I Bsu36I ClaI
DrdI EagI EciI EcoRV FseI FspAI HincII HpaI
KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PasI PciI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SalI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SwaI TstI XbaI XhoI XmaI