Restriction map for
pF1KB3618
(Linear) (Six-base) MAPSORT of: KIBB3618.seq Check: 8404 from: 1 to: 885
KIBB3618.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 634 688 885
Size: 109 525 54 197
Fragments arranged by size:
525 197 109 54
AfeI AGC'GCT
Cuts at: 0 721 885
Size: 721 164
ApaI G_GGCC'C
Cuts at: 0 881 885
Size: 881 4
BanII G_rGCy'C
Cuts at: 0 439 881 885
Size: 439 442 4
Fragments arranged by size:
442 439 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 116 642 885
Size: 116 526 243
Fragments arranged by size:
526 243 116
BbvCI CC'TCA_GC
Cuts at: 0 376 885
Size: 376 509
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 411 445 885
Size: 411 34 440
Fragments arranged by size:
440 411 34
BclI T'GATC_A
Cuts at: 0 629 885
Size: 629 256
BglI GCCn_nnn'nGGC
Cuts at: 0 766 885
Size: 766 119
BglII A'GATC_T
Cuts at: 0 540 885
Size: 540 345
Bme1580I G_kGCm'C
Cuts at: 0 881 885
Size: 881 4
BmrI ACTGGGnnnn_n'
Cuts at: 0 174 508 693 885
Size: 174 334 185 192
Fragments arranged by size:
334 192 185 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 283 799 885
Size: 97 186 516 86
Fragments arranged by size:
516 186 97 86
Bpu10I CC'TnA_GC
Cuts at: 0 64 376 885
Size: 64 312 509
Fragments arranged by size:
509 312 64
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 885
Size: 840 45
BsaBI GATnn'nnATC
Cuts at: 0 760 885
Size: 760 125
BsaHI Gr'CG_yC
Cuts at: 0 731 885
Size: 731 154
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 259 289 885
Size: 25 234 30 596
Fragments arranged by size:
596 234 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 885
Size: 280 605
BseYI C'CCAG_C
Cuts at: 0 295 534 831 885
Size: 295 239 297 54
Fragments arranged by size:
297 295 239 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 885
Size: 377 508
BsiEI CG_ry'CG
Cuts at: 0 19 885
Size: 19 866
BsiHKAI G_wGCw'C
Cuts at: 0 65 885
Size: 65 820
BsmI GAATG_Cn'
Cuts at: 0 187 287 885
Size: 187 100 598
Fragments arranged by size:
598 187 100
Bsp1286I G_dGCh'C
Cuts at: 0 65 439 881 885
Size: 65 374 442 4
Fragments arranged by size:
442 374 65 4
BsrBI CCG'CTC
Cuts at: 0 21 885
Size: 21 864
BstAPI GCAn_nnn'nTGC
Cuts at: 0 544 885
Size: 544 341
BstYI r'GATC_y
Cuts at: 0 540 885
Size: 540 345
EaeI y'GGCC_r
Cuts at: 0 564 885
Size: 564 321
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 790 885
Size: 790 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 109 283 634 688 799 885
Size: 97 12 174 351 54 111 86
Fragments arranged by size:
351 174 111 97 86 54 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 781 802 885
Size: 781 21 83
Fragments arranged by size:
781 83 21
EcoO109I rG'GnC_Cy
Cuts at: 0 68 196 510 878 885
Size: 68 128 314 368 7
Fragments arranged by size:
368 314 128 68 7
HaeII r_GCGC'y
Cuts at: 0 723 885
Size: 723 162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 259 291 885
Size: 27 232 32 594
Fragments arranged by size:
594 232 32 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 885
Size: 227 658
MscI TGG'CCA
Cuts at: 0 566 885
Size: 566 319
MspA1I CmG'CkG
Cuts at: 0 87 456 885
Size: 87 369 429
Fragments arranged by size:
429 369 87
PflMI CCAn_nnn'nTGG
Cuts at: 0 810 885
Size: 810 75
PfoI T'CCnGG_A
Cuts at: 0 776 885
Size: 776 109
PpuMI rG'GwC_Cy
Cuts at: 0 68 510 885
Size: 68 442 375
Fragments arranged by size:
442 375 68
PspOMI G'GGCC_C
Cuts at: 0 877 885
Size: 877 8
PstI C_TGCA'G
Cuts at: 0 341 885
Size: 341 544
PvuII CAG'CTG
Cuts at: 0 456 885
Size: 456 429
ScaI AGT'ACT
Cuts at: 0 682 885
Size: 682 203
SexAI A'CCwGG_T
Cuts at: 0 447 885
Size: 447 438
SfcI C'TryA_G
Cuts at: 0 337 885
Size: 337 548
SmlI C'TyrA_G
Cuts at: 0 819 885
Size: 819 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 354 885
Size: 354 531
TatI w'GTAC_w
Cuts at: 0 680 885
Size: 680 205
Tth111I GACn'n_nGTC
Cuts at: 0 449 885
Size: 449 436
Enzymes that do cut:
AcuI AfeI ApaI BanII BbsI BbvCI BcgI BclI
BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BsrBI BstAPI BstYI EaeI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I MmeI MscI MspA1I PflMI PfoI
PpuMI PspOMI PstI PvuII ScaI SexAI SfcI SmlI
TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BciVI BfrBI BlpI BmgBI BmtI BplI BsaI BsaAI
BsaWI BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TstI XbaI XcmI XhoI XmaI XmnI
ZraI