(Linear) (Six-base) MAPSORT of: KIBB3614.seq  Check: 1794  from: 1  to: 1254



KIBB3614.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AflIII A'CryG_T

Cuts at:      0    1178    1254 
   Size:       1178      76
 
AlwNI CAG_nnn'CTG

Cuts at:      0     298    1254 
   Size:        298     956
 
ApoI r'AATT_y

Cuts at:      0     602     765    1074    1254 
   Size:        602     163     309     180

  Fragments arranged by size: 

                 602     309     180     163
 
AvrII C'CTAG_G

Cuts at:      0     673    1254 
   Size:        673     581
 
BanII G_rGCy'C

Cuts at:      0    1092    1254 
   Size:       1092     162
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     740    1106    1254 
   Size:        740     366     148

  Fragments arranged by size: 

                 740     366     148
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      89    1254 
   Size:         89    1165
 
BsaHI Gr'CG_yC

Cuts at:      0     501    1254 
   Size:        501     753
 
BsaWI w'CCGG_w

Cuts at:      0      31     710    1254 
   Size:         31     679     544

  Fragments arranged by size: 

                 679     544      31
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1034    1064    1254 
   Size:       1034      30     190

  Fragments arranged by size: 

                1034     190      30
 
BsmI GAATG_Cn'

Cuts at:      0     647    1254 
   Size:        647     607
 
Bsp1286I G_dGCh'C

Cuts at:      0    1092    1254 
   Size:       1092     162
 
BspHI T'CATG_A

Cuts at:      0     665    1254 
   Size:        665     589
 
BstYI r'GATC_y

Cuts at:      0     583    1133    1254 
   Size:        583     550     121

  Fragments arranged by size: 

                 583     550     121
 
BtgI C'CryG_G

Cuts at:      0     496    1254 
   Size:        496     758
 
DraI TTT'AAA

Cuts at:      0    1206    1254 
   Size:       1206      48
 
EarI CTCTTCn'nnn_

Cuts at:      0      55     196    1097    1254 
   Size:         55     141     901     157

  Fragments arranged by size: 

                 901     157     141      55
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     740    1106    1254 
   Size:        740     366     148

  Fragments arranged by size: 

                 740     366     148
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     210    1254 
   Size:        210    1044
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      26     575     607    1254 
   Size:         26     549      32     647

  Fragments arranged by size: 

                 647     549      32      26
 
HincII GTy'rAC

Cuts at:      0     432    1254 
   Size:        432     822
 
MfeI C'AATT_G

Cuts at:      0     881    1254 
   Size:        881     373
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     512     795    1254 
   Size:        512     283     459

  Fragments arranged by size: 

                 512     459     283
 
MspA1I CmG'CkG

Cuts at:      0     483    1254 
   Size:        483     771
 
NcoI C'CATG_G

Cuts at:      0     496    1254 
   Size:        496     758
 
NspI r_CATG'y

Cuts at:      0     942    1182    1254 
   Size:        942     240      72

  Fragments arranged by size: 

                 942     240      72
 
PciI A'CATG_T

Cuts at:      0    1178    1254 
   Size:       1178      76
 
PvuII CAG'CTG

Cuts at:      0     483    1254 
   Size:        483     771
 
SapI GCTCTTCn'nnn_

Cuts at:      0      55     196    1254 
   Size:         55     141    1058

  Fragments arranged by size: 

                1058     141      55
 
SfcI C'TryA_G

Cuts at:      0     587    1254 
   Size:        587     667
 
SmlI C'TyrA_G

Cuts at:      0     104    1254 
   Size:        104    1150
 
StyI C'CwwG_G

Cuts at:      0     496     673    1007    1254 
   Size:        496     177     334     247

  Fragments arranged by size: 

                 496     334     247     177
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     229     987    1254 
   Size:        229     758     267

  Fragments arranged by size: 

                 758     267     229
 
TatI w'GTAC_w

Cuts at:      0     518    1254 
   Size:        518     736
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     911     943    1034    1066    1254 
   Size:        911      32      91      32     188

  Fragments arranged by size: 

                 911     188      91      32      32
 
XbaI T'CTAG_A

Cuts at:      0      45    1254 
   Size:         45    1209
 

 Enzymes that do cut:

   AflIII    AlwNI     ApoI    AvrII    BanII     BpmI    BpuEI    BsaHI
    BsaWI    BsaXI     BsmI Bsp1286I    BspHI    BstYI     BtgI     DraI
     EarI  Eco57MI     FalI    Hin4I   HincII     MfeI     MmeI   MspA1I
     NcoI     NspI     PciI    PvuII     SapI     SfcI     SmlI     StyI
    TaqII     TatI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI
    BsaBI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MluI     MscI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI